Prevalence and Molecular Epidemiological Typing of Penicillinase-Producing Neisseria gonorrhoeae and Their blaTEM-135 Gene Variants in Nanjing, China

Chen, Shaochun; Yin, Yue-Ping; Dai, Xiu-Qin; Yu, Ruixing; Han, Yang; Sun, Houhua; Ohnishi, Makoto; Unemo, Magnus; Chen, Xiang-Sheng

doi:10.1097/olq.0000000000000037

Cited by 21 publications

(21 citation statements)

References 20 publications

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“…Only one K. pneumoniae harbored a TEM-ESBL (TEM-135) subtype, which has also been reported in other parts of China. 43 As reported in other previous works, the OXA-1 subtypes were co-harbored along with other enzymes. 10 Statistical evaluation of our data revealed that bla TEM and bla CTX-M-1 group genes were positively associated with resistance to more than three β-lactams.…”

Section: Discussionsupporting

confidence: 66%

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

et al. 2015

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Resistance to β-lactam antibiotics through β-lactamase production by Enterobacteriaceae continues to burden the health-care sector worldwide. Traditional methods for detection of β-lactamases are time-consuming and labor-intensive and newer methods with varying capabilities continue to be developed. The objective of this study was to develop a multiplex PCR (M-PCR) system for the detection of bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes and to apply it in clinical Klebsiella pneumoniae and Escherichia coli strains. To do this, we used group-specific PCR primers in singleplex reactions followed by optimization into multiplex reactions. Specificity and sensitivity of the M-PCR were then evaluated using 58 reference strains before its application to detect bla group genes in 203 clinical Enterobacteriaceae strains. PCR amplicons were sequenced to determine the β-lactamase subtypes. The M-PCR system exhibited 100% specificity and sensitivity. In all, 83.7% of K. pneumoniae and 89.8% of E. coli clinical strains harbored bla group genes with 46.9%, 40.1%, 15.0%, 21.1% and 6.1% of K. pneumoniae having bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively, whereas 12.2%, 77.6%, 22.4%, 36.7% and 8.2% of E. coli had bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively. Bla SHV-1 , bla SHV-11 , bla SHV-27 , bla SHV-33 , bla SHV-144 , bla TEM-1 , bla TEM-135 , bla OXA-1 , bla CTX-M-3 , bla CTX-M-9 , bla CTX-M-14 , bla CTX-M-15 , bla CTX-M-27 , bla CTX-M-55 , bla CTX-M-65 and bla CTX-M-104 were detected. In conclusion, the M-PCR system was efficient and versatile with an advantage of simultaneously detecting all the targeted bla group genes. Hence, it is a potential candidate for developing M-PCR kits for the screening of these genes for clinical or epidemiological purposes.

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Section: Discussionsupporting

confidence: 66%

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

et al. 2015

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“…No extended-spectrum ␤-lactamase (ESBL) has yet been acquired or developed in gonococci. Nevertheless, in many currently circulating strains, the bla TEM-135 gene, which differs by one single nucleotide polymorphism (SNP) from bla TEM-1 , has been found, and only one additional SNP could result in an ESBL capable of hydrolyzing and degrading ESCs (183)(184)(185).…”

Section: Penicillin Resistancementioning

confidence: 99%

Antimicrobial Resistance in Neisseria gonorrhoeae in the 21st Century: Past, Evolution, and Future

2014

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SUMMARY Neisseria gonorrhoeae is evolving into a superbug with resistance to previously and currently recommended antimicrobials for treatment of gonorrhea, which is a major public health concern globally. Given the global nature of gonorrhea, the high rate of usage of antimicrobials, suboptimal control and monitoring of antimicrobial resistance (AMR) and treatment failures, slow update of treatment guidelines in most geographical settings, and the extraordinary capacity of the gonococci to develop and retain AMR, it is likely that the global problem of gonococcal AMR will worsen in the foreseeable future and that the severe complications of gonorrhea will emerge as a silent epidemic. By understanding the evolution, emergence, and spread of AMR in N. gonorrhoeae , including its molecular and phenotypic mechanisms, resistance to antimicrobials used clinically can be anticipated, future methods for genetic testing for AMR might permit region-specific and tailor-made antimicrobial therapy, and the design of novel antimicrobials to circumvent the resistance problems can be undertaken more rationally. This review focuses on the history and evolution of gonorrhea treatment regimens and emerging resistance to them, on genetic and phenotypic determinants of gonococcal resistance to previously and currently recommended antimicrobials, including biological costs or benefits; and on crucial actions and future advances necessary to detect and treat resistant gonococcal strains and, ultimately, retain gonorrhea as a treatable infection.

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“…These studies also highlighted that bla TEM-135 may occur in distinct genetic backgrounds and suggested that the gene has continued to be shared among gonococci via horizontal genetic exchange, having been found on the Asian and Rio/Toronto plasmid types and across various multilocus sequence types (MLST) and N. gonorrhoeae multiantigen sequence types (NG-MAST) (8,11,12,14).…”

mentioning

confidence: 99%

“…Data on the rate at which penicillinase-producing N. gonorrhoeae (PPNG) isolates carry the bla TEM-135 gene are limited, ranging from 9.4% (9/96 PPNG isolates) in Thailand (9) and 20% (2/10 PPNG isolates) in Japan (12) to 58% (66/114 PPNG isolates) in China (11). These studies also highlighted that bla TEM-135 may occur in distinct genetic backgrounds and suggested that the gene has continued to be shared among gonococci via horizontal genetic exchange, having been found on the Asian and Rio/Toronto plasmid types and across various multilocus sequence types (MLST) and N. gonorrhoeae multiantigen sequence types (NG-MAST) (8,11,12,14).…”

mentioning

confidence: 99%

Penicillinase-Producing Plasmid Types in Neisseria gonorrhoeae Clinical Isolates from Australia

Whiley

Trembizki

Buckley

et al. 2014

Antimicrob Agents Chemother

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h Penicillinase-producing Neisseria gonorrhoeae (PPNG) carrying the bla TEM-135 gene is of particular concern, as it is considered a stepping stone toward resistance to extended-spectrum cephalosporins. Here, we sought to characterize plasmid types and the occurrence of the bla TEM-135 gene for N. gonorrhoeae clinical isolates from Australia. We found that bla TEM-135 was prevalent in Australian PPNG and was detected on all three major plasmid types. High-level resistance to penicillin in Neisseria gonorrhoeae is conferred by plasmid-mediated penicillinase production. To date, seven different penicillinase-harboring gonococcal plasmid types have been described and comprise three main plasmid types (Asian, African, and Rio/Toronto plasmids) and four less common variants (Nimes, New Zealand, Johannesburg, and Australian plasmids) (1-7). There are two different penicillinase genes that may be carried by these plasmids, including the classical bla TEM-1 gene and the more recently described gonococcal bla TEM-135 gene (8). The bla gene is of concern as it is considered to be an intermediate between a penicillinase and an extended-spectrum ␤-lactamase (9) and is therefore considered a potential future threat to the effectiveness of N. gonorrhoeae treatment. The bla TEM-135 gene was first described in an isolate from Thailand (8) and has since been reported in various countries, including Canada (10), China (11), Japan (12), and Switzerland (13). However, there are now more recent data showing that bla has been present in gonococci since at least 1984, suggesting that the gene is not, as originally thought, a recent evolutionary event driven by selective pressure through the use of extended-spectrum cephalosporins (14).Data on the rate at which penicillinase-producing N. gonorrhoeae (PPNG) isolates carry the bla TEM-135 gene are limited, ranging from 9.4% (9/96 PPNG isolates) in Thailand (9) and 20% (2/10 PPNG isolates) in Japan (12) to 58% (66/114 PPNG isolates) in China (11). These studies also highlighted that bla TEM-135 may occur in distinct genetic backgrounds and suggested that the gene has continued to be shared among gonococci via horizontal genetic exchange, having been found on the Asian and Rio/Toronto plasmid types and across various multilocus sequence types (MLST) and N. gonorrhoeae multiantigen sequence types (NG-MAST) (8,11,12,14).In this study, we sought to characterize plasmid types and the occurrence of the bla TEM-135 gene in N. gonorrhoeae clinical isolates from Australia. A total of 351 PPNG clinical isolates were included in this study (Table 1). These were all PPNG isolates identified as part of a broader investigation of N. gonorrhoeae isolates (n ϭ 2,455) that were collected from throughout Australia in the first half of the year 2012 (7, 15). The isolates were initially determined to be PPNG on the basis of acidometric and nitrocefin disc testing and subsequently by real-time PCR (7). The DNA used for the real-time PCR testing was stored at Ϫ80°C until further investigation. Here, the DNA ...

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Prevalence and Molecular Epidemiological Typing of Penicillinase-Producing Neisseria gonorrhoeae and Their blaTEM-135 Gene Variants in Nanjing, China

Cited by 21 publications

References 20 publications

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

Antimicrobial Resistance in Neisseria gonorrhoeae in the 21st Century: Past, Evolution, and Future

Penicillinase-Producing Plasmid Types in Neisseria gonorrhoeae Clinical Isolates from Australia

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