Prevalence and molecular identification of Sarcocystis spp.infecting water buffaloes (Bubalus bubalus) in Sharkia Province, Egypt.

Ras, Refaat

doi:10.21608/evmspj.2021.142812

Cited by 4 publications

(7 citation statements)

References 30 publications

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“…Higher prevalence of 87% in India (35) and 94%, 85.96%, and 82.3% in Egypt (36, 16, 2) were detected. Older buffaloes had a significantly higher prevalence than younger ones similar to the previous reports in Vietnam (30) and Egypt (16,17). This may be due to prolonged exposure to infections (17).…”

Section: Discussionsupporting

confidence: 86%

“…Older buffaloes had a significantly higher prevalence than younger ones similar to the previous reports in Vietnam (30) and Egypt (16,17). This may be due to prolonged exposure to infections (17). Other authors reported a slight variation in prevalence in India (12) and Egypt (2).…”

Section: Discussionsupporting

confidence: 83%

“…The prevalence was similar to 65% in the Philippines (27) and 68.2%, The tree was drawn using the Neighbor-Joining method and provided with a scale bar 69%, and 74% in Egypt (8, 28, 29). Our prevalence was higher than 41% in Vietnam (30), 22.62% in India (11), 15.6% in Iraq (31), and 8.33%, 28%, 41.5%, 42%, and 58.72% in Egypt (32,33,34,17,15). Higher prevalence of 87% in India (35) and 94%, 85.96%, and 82.3% in Egypt (36, 16, 2) were detected.…”

Section: Discussionmentioning

confidence: 54%

“…This was consistent with the results reported in Egypt (32,34) and may be due to prolonged exposure to infection because of aging and exposure to stress factors like pregnancy and lactation that suppress the immune system (29). While the prevalence in males was higher than in females in Egypt (17), this may be due to the increased number of investigated males. Interestingly, gender did not affect the prevalence of Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 82%

“…infections with varying prevalence rates, including Northern Vietnam (9), Iran (10,11), India (12), China (13), and Ethiopia (14). In Egypt, infection was detected in water buffalos from a few provinces such as Beni-Suef (2), El-Gharbia (8), Dakahlia (15), Menoufia (16), and Sharkia (17). The serological diagnosis was conducted using ELISA in water buffaloes from Iran (12).…”

Section: Introductionmentioning

confidence: 99%

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PREVALENCE AND GENETIC CHARACTERIZATION OF Sarcocystis fusiformis IN WATER BUFFALOES (Bubalus bubalis) IN TWO NORTHERN PROVINCES OF EGYPT

Menshawy

Essa²,

Shaaban³

et al. 2023

SVR

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Sarcocystosis considerably occurs in a wide host range including animals, reptiles, humans, and birds. This study was conducted to determine the prevalence of Sarcocystis spp. using abattoir inspection, genetic characterization, as well as histopathology in water buffaloes in two provinces, Elbehera and Kafrelsheikh, Egypt. Specimens were collected from esophagus, tongue, and masseters of 400 slaughtered buffaloes in Elbehera (n= 215) and Kafrelsheikh (n= 185). Samples were examined macroscopically and histopathologically. Furthermore, genetic characterization of Sarcocystis spp. was performed using the 18SrRNA gene-based PCR. The total prevalence was 71.0% (75.3% and 65.9% in Elbehera and Kafrelsheikh, respectively). Aged buffaloes had a higher prevalence than young ones. Females had a higher prevalence than males. The esophagus was the most infected organ. Molecular analysis revealed that the recovered species was S. fusiformis. This is the first genetic characterization of S. fusiformis in water buffaloes from Elbehera and Kafrelsheikh, Egypt. Higher prevalence proposed the potential role of cats in the transmission of S. fusiformis, which, in turn, requires strict hygienic measures to protect animals and humans from infection.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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PREVALENCE AND GENETIC CHARACTERIZATION OF Sarcocystis fusiformis IN WATER BUFFALOES (Bubalus bubalis) IN TWO NORTHERN PROVINCES OF EGYPT

Menshawy

Essa²,

Shaaban³

et al. 2023

SVR

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Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes

Barakat,

Abdel-Aty,

Ibrahim

et al. 2023

Sci Rep

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Sarcocystis spp. infects water buffaloes (Bubalus bubalis) causing sarcocystosis. In the present study, Sarcocystis fusiformis was recognized in Egyptian water buffaloes based on histological observation and molecular analysis of internal transcribed spacer 1 (ITS1), 18S ribosomal RNA (18S rRNA) and cytochrome c oxidase subunit I (COX-1) gene fragments. Chemotherapy and vaccines against Sarcocystis spp. could potentially target proteases because they may play a crucial role in the infection. Cysteine proteases are multifunctional enzymes involved in vital metabolic processes. However, the involvement of proteases in S. fusiform infection has not yet been characterized. Here, the purification and study on some biochemical properties of protease isolated from cysts of S. fusiform were carried out. Protease with a molecular weight of 100 kDa was purified. LC–MS/MS analyzed the protein sequence of purified protease and the data suggested that the enzyme might be related to the cysteine protease. The purified protease exhibited maximum activity at pH 6 and a temperature of 50 °C. The Michaelis–Menten constant (Km), the maximum velocity (Vmax), and the turnover number (Kcat) were determined. The complete inhibition effect of cysteine inhibitors indicated that the purified enzyme is a cysteine protease. The results suggested that S. fusiform proteolytic enzyme may be necessary for parasite survival in water buffaloes by digesting host tissues. Therefore, cysteine protease could be a suitable target for vaccinations.

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Infection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt

Elsharkawy,

Barghash,

El-Nour

et al. 2024

BMC Vet Res

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Background Sarcocystosis is a parasitic disease found worldwide, resulting from various Sarcocystis species. The current research was carried out in three significant economic areas in Egypt: Greater Cairo, the Nile Delta, and Upper Egypt. It aimed to investigate the occurrence of Sarcocystis spp. in locally bred water buffaloes Bubalus bubalis. Methods To achieve this objective, 317 buffalos were slaughtered in different slaughterhouses in various regions of Egypt. Samples of heart, skeletal muscle, esophagus, and tongue were assessed using macroscopic and microscopic tests. Examination methods included direct optical observation of tissues as well as digestion and examination of the sediment obtained from the tissues. Additionally, ultrastructural features were analyzed using scanning and transmission electron microscopy. Molecular characterization was conducted through PCR, followed by nucleotide sequencing and phylogenetic analysis. Results A total of 317 slaughtered buffaloes were examined for Sarcocystis during the period from September 2021 to October 2023. The prevalence of infection was recorded with 229 out of 317 (72.2%) infected with Sarcocystis spp. The results also showed that the prevalence of Sarcocystis species in females was higher than males. Based on the age of carcasses, adults (> 2 years) had a higher infection rate compared to young ones (< 2 years). Regarding seasonal variation, the highest prevalence of infection was recorded during the summer followed by spring, and then autumn, while winter had the lowest prevalence of infection. Additionally, the skeletal muscle was the most susceptible organ to sarcocystosis (87.3%) followed by the esophageal muscle (8.3%), the tongue (4.4%), and no infection in the heart muscle. The use of scanning and transmission electron microscopy allowed the identification of S. fusiformis and S. cruzi in buffaloes in Egypt. Furthermore, the Sarcocystis 18 S rRNA genes from skeletal tissue samples were cloned and sequenced under accession numbers OQ507387, OQ507388, and OQ507389 for S. fusiforms, and one OQ507391 for S. cruzi. Conclusion The findings revealed a notably high prevalence of Sarcocystis infection (72.2%) in buffaloes from Egypt, with skeletal muscle identified as the organ most susceptible to the parasite. Two Sarcocystis species were detected: S. fusiformis and S. cruzi.

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Prevalence and molecular identification of Sarcocystis spp.infecting water buffaloes (Bubalus bubalus) in Sharkia Province, Egypt.

Cited by 4 publications

References 30 publications

PREVALENCE AND GENETIC CHARACTERIZATION OF Sarcocystis fusiformis IN WATER BUFFALOES (Bubalus bubalis) IN TWO NORTHERN PROVINCES OF EGYPT

PREVALENCE AND GENETIC CHARACTERIZATION OF Sarcocystis fusiformis IN WATER BUFFALOES (Bubalus bubalis) IN TWO NORTHERN PROVINCES OF EGYPT

Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes

Infection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt

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