Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia

Beck, Relja; Sprong, Hein; Bata, Ingeborg; Lučinger, Snježana; Pozio, Edoardo; Cacciò, Simone M.

doi:10.1016/j.vetpar.2010.09.026

Cited by 57 publications

(45 citation statements)

References 29 publications

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“…also, there is a report of Giardia sp. infection in captive felines from the Croatian Zagreb ZOO (BECK et al, 2011b) and in domestic cats (CaCCIÒ et al, 2010). the low prevalence of Giardia sp.…”

Section: Resultsmentioning

confidence: 99%

Endoparasites of wildcats in Croatia

et al. 2017

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ABSTRACTreports on the parasitic fauna of wildcats (Felis silvestris silvestris) are rare and often based on a small sample size, therefore the goal of this research was to investigate the prevalence of endoparasites in wildcats in Croatia. Necropsy was conducted on 34 adult wildcats killed in traffic or provided by hunters following regular hunting operations. all animals tested negative for rabies. the contents of the stomach and intestine were examined under a microscope. Feces from the rectum were analyzed using flotation with a saturated ZnSO 4 solution, while the diaphragm was examined using artificial digestion. Direct immunofluorescence was used for the first time to detect Giardia sp. cysts in wildcats. all animals were infected with at least one species of parasites, while the most diverse infestation included six different species of parasites in a single animal. the following parasite species were found (% of prevalence of adult parasites and their developmental stages in all analyzed samples): Taenia

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Section: Resultsmentioning

confidence: 99%

Endoparasites of wildcats in Croatia

et al. 2017

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“…Not discussed Croatia, India, Philippines (Chu, 1930;Abraham, 1962;Beck et al, 2011) Z, zoonotic potential acknowledged; M, protozoan affects multiple hosts other than Viverridae. …”

Section: Common Genetmentioning

confidence: 99%

Potential Pathogens Reported in Species of the Family Viverridae and Their Implications for Human and Animal Health

Wicker

Canfield

Higgins

2016

Zoonoses and Public Health

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Impacts• First collation of previously published organisms isolated from species of the family Viverridae provides baseline information to assist viverrid population management.• Summarizes anthropogenic factors (trade, farming, encroachment on viverrid habitat) bringing viverrids into proximity with humans.• Discusses detected organisms in terms of potential risk for public health given the described anthropogenic factors which facilitate transmission and spillover of organisms between and amongst animals and people. SummaryThe Viverridae is a family of nocturnal carnivores including civets, genets and African linsangs. While a list of known organisms isolated from a species is an essential tool for population management, this review represents the first attempt to collate published reports of organisms isolated from viverrids. A wide range of organisms, including 11 viruses, eight bacterial species, one internal arthropod species, representatives from eight genera of protozoan, 21 genera of nematode, seven genera of cestode, eight genera of trematode and six genera of external arthropod (mites, ticks and louse), have been reported in literature spanning over a century of research. Many of these are capable of infecting multiple hosts, including humans. This is of concern given the anthropogenic factors that bring humans and domestic species into close contact with viverrids, facilitating transmission and spillover of organisms between groups. These factors include trade in viverrids for human consumption, captive management in zoos, rescue centres or on commercial breeding farms, and the increasing overlap of free-ranging viverrid distribution and human settlement.

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“…G. lamblia, Giardia intestinalis), G. agilis, G. muris, G, psittaci, G. ardeae, and G. microti, distinguished on the basis of the morphology and ultrastructure of their trophozoites (Sprong et al 2009). In any case, it is well established that G. duodenalis is a species complex composed of at least seven distinct genetic groups, referred as assemblages A to G (Abe et al 2010;Beck et al 2011;Lasek-Nesselquist et al 2009;Lebbad et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and genetic characterization of Giardia duodenalis from captive nonhuman primates by multi-locus sequence analysis

et al. 2011

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Giardia is the most common enteric protozoan that can be pathogenic to both humans and animals. Transmission can be direct through the faecal-oral route, or through ingestion of contaminated water or food. Genetic characterization of Giardia duodenalis isolates has demonstrated the existence of seven groups (assemblages A to G) which differ in their host distribution. Assemblages A and B are present in humans and other primates, dogs, cats, rodents, and other species of wild mammals, but the role of the different host animals in the epidemiology of human infection remains unclear. With this preliminary data, we can infer that nonhuman primates (NHP) might be a potential reservoir for zoonotic transmission. This research paper discusses the presence of Giardia in nonhuman primates housed in two Spanish zoological gardens (located in Valencia and Madrid). Twenty faecal samples obtained from 16 different species of NHP were studied; 70% were positives to Giardia, and genetic analyses were performed by sequencing of four genes (SSrRNA, glutamate dehydrogenase, triose phosphate isomerase, and beta-giardin). The assemblage A was the most frequent (63.4%) in the species studied. A sequence from a red ruffed lemur (corresponding to genotype AI) was obtained, and this is the first reported sequence of a gdh gene obtained from this species. The multi-locus sequence analysis was also performed on the samples positive to nested PCR belonging to assemblage B. After amplification using the GDHeF, GDHiF, and GDHiR gdh primers; AL3543, AL3546, AL3544, and AL3545 tpi primers; G7, G759, GBF, and GBR bg primers, amplicons of 432, 500, and 511 bp respectively were obtained. Amplification products were sequenced and the sequence and phylogenetic analyses showed that genotype IV like was the most frequent in the samples belonging to this assemblage.

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Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia

Cited by 57 publications

References 29 publications

Endoparasites of wildcats in Croatia

Endoparasites of wildcats in Croatia

Potential Pathogens Reported in Species of the Family Viverridae and Their Implications for Human and Animal Health

Occurrence and genetic characterization of Giardia duodenalis from captive nonhuman primates by multi-locus sequence analysis

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