Prevalence and morphological and molecular characteristics of <i>Sarcocystis bertrami</i> in horses in China

Ma, Chaoyang; Ye, Yulong; Wen, Tao; Huang, Zhumei; Pan, Jianguo; Hu, Junjie; Tao, Jianping; Song, Jiakun

doi:10.1051/parasite/2019078

Cited by 16 publications

(15 citation statements)

References 16 publications

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“…A possible explanation was supposed by Ma et al . (2020) for the high similarities with S. fayeri and S. bertrami is that both represent the same species of Sarcocystis in horses.…”

Section: Resultsmentioning

confidence: 95%

“…Nucleotide sequence analysis has proven to be a useful genetic tool for delineating Sarcocystis species from same or different host species (Ma et al . 2020). In the present study, a total of 799 bp has been deposited under accession no.…”

Section: Resultsmentioning

confidence: 99%

“…Infection with Sarcocystis is considered to be a common protozoan in horses (Ma et al . 2020). They are intermediate hosts for S. bertrami , S. neurona , S. equicanis and S. fayeri (Juyal et al .…”

Section: Introductionmentioning

confidence: 99%

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Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence

Abdel‐Gaber

Quraishy

Dkhil

et al. 2020

Lett Appl Microbiol

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Sarcocystosis is a parasitic disease caused by an intracellular protozoan parasite Sarcocystis belonging to the phylum Apicomplexa. These parasites have a requisite two‐host life cycle. Recently, there are many Sarcocystis species that identified morphologically. In the present study, diaphragmatic muscle samples from the domestic horse (Equus caballus) were examined for Sarcocystis infection. The natural infection with sarcocysts was recorded to be 62·5% for only microcysts in the infected muscles. Molecular analysis using the 18S rRNA gene was conducted to swiftly and accurately identify the recovered species. Studies on the expression of the 18S rRNA gene have confirmed that the present parasite isolates belong to the Sarcocystis genus. The sequence data showed significant identities (>80%) with archived gene sequences from species within the Sarcocystidae family, and a dendrogram showing the phylogenetic relationship was constructed. The most closely related species were the previously described Sarcocystis fayeri and Sarcocystis bertrami. The current data showed that the present species was identified as S. fayeri and deposited in GenBank (accession number MF614956.1). This study highlights the importance of the genetic data in the exact taxonomy within sarcocystid species.

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“…A possible explanation was supposed by Ma et al . (2020) for the high similarities with S. fayeri and S. bertrami is that both represent the same species of Sarcocystis in horses.…”

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence

Abdel‐Gaber

Quraishy

Dkhil

et al. 2020

Lett Appl Microbiol

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“…The morphological characteristics of horse sarcocysts have been observed to undergo changes in various stages of development, showing long protrusions in the early phase and short protrusions in the later phase (Fayer et al 1983). Therefore, there is still considerable confusion concerning the relationship between S. bertrami/equicanis and S. fayeri (Odening et al 1995;Ma et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The morphological and molecular characteristics of S. bertrami sarcocysts isolated from horses in China have been investigated by our group previously (Ma et al 2020). To clarify the relationship of the Sarcocystis species of donkeys and horses based on the molecular identi cation of meat samples, the aims of the present study were to investigate the morphological characteristics of donkey sarcocysts and to explore the relationship of the Sarcocystis species of donkeys and horses via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and phylogenetic analysis.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characteristics of Sarcocystis sp. in donkeys from China

Zhang

Wei

et al. 2022

Preprint

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There is considerable confusion concerning the relationships among species of Sarcocystis found in donkeys and horses. Here, sarcocysts obtained from donkeys (Equus asinus) were morphologically and molecularly characterized. Sarcocysts were diagnosed in 12 of 32 (37.5%) Chinese donkeys and could be divided into two types, thin-walled and thick-walled, with the aid of light microscopy (LM). The thin-walled sarcocysts were macroscopic (up to 4856 × 320 µm in size) and had short club-like protrusions of up to 2.7 µm; the thick-walled sarcocysts were microscopic (up to 3750 × 135 µm in size) and had villar protrusions of up to 5.4 µm. The ultrastructures of the two types exhibited highly similar morphological characteristics, including bundled microtubules in the core of the villar protrusions penetrating diagonally into the ground substance, similar to wall type 11c. Three genetic markers, 18S rDNA, 28S rDNA, and mitochondrial cox1, were sequenced and analyzed in the two types. The three genetic markers presented high intraspecific similarity between the two types, ranging from 97.2–99.5%, 97.8–99.6% and 99.0 − 99.9%, respectively. The comparison of these sequences with those of Sarcocystis spp. previously deposited in GenBank showed that the newly obtained sequences of 18S rDNA, 28S rDNA, and mitochondrial cox1 presented identities of 90.0 − 97.5%, 94.7 − 95.1% and 82.6 − 84.5%, respectively, with those of S. bertrami and S. fayeri obtained from horses. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based on the mitochondrial cox1 sequences of donkey and horse sarcocysts revealed three fragments (196, 243 and 641 bp) and two fragments (416 and 644 bp), respectively, upon digestion with the restriction enzymes EcoRI and HinfI. Phylogenetic analysis based on 18S rDNA or mitochondrial cox1 sequences revealed that donkey sarcocysts formed an individual clade with Sarcocystis spp. obtained from horses. Based on the divergence of the three sarcocyst genetic markers, especially mitochondrial cox1, between donkeys and horses, the Sarcocystis sp. sarcocysts in donkeys were different with those obtained from horses. The cross-infection of Sarcocystis between donkeys and horses needs to be attempted for further confirmation in the future.

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Morphological and molecular characterization of a Sarcocystis species infecting donkeys from China

Zhang

Wei

et al. 2022

Parasitol Res

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Prevalence and morphological and molecular characteristics of Sarcocystis bertrami in horses in China

Cited by 16 publications

References 16 publications

Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence

Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence

Morphological and molecular characteristics of Sarcocystis sp. in donkeys from China

Morphological and molecular characterization of a Sarcocystis species infecting donkeys from China

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