Prevalence and multilocus genotyping of Giardia duodenalis in zoo animals in three cities in China

Zou, Yang; Li, Xiaodong; Meng, Yu-Meng; Wang, Xiaolong; Wang, Hao-Ning; Zhu, Xing‐Quan

doi:10.1007/s00436-022-07565-w

Cited by 7 publications

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these, G. duodenalis is the most commonly reported species in humans and animals. G. duodenalis is considered as a complex species and has been further categorized into eight assemblages (A–H) based on genetic analysis [ 22 ]. Assemblages A and B have a wide host range and are responsible for human infections [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and Molecular Characterization of Zoonotic Gastrointestinal Protozoal Infection in Zoo Animals in China

An,

Jiang,

Zhang

et al. 2024

Animals

View full text Add to dashboard Cite

Zoo animals, harboring zoonotic gastrointestinal protozoal diseases, pose potential hazards to the safety of visitors and animal keepers. This study involved the collection and examination of 400 fresh fecal samples from 68 animal species, obtained from five zoos. The aim of this study was to determine the occurrence, genetic characteristics, and zoonotic potential of common gastrointestinal protists. PCR or nested PCR analysis was conducted on these samples to detect four specific parasites: Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis spp. The overall prevalence of Cryptosporidium spp was 0.5% (2/400), G. duodenalis was 6.0% (24/400), Blastocystis spp. was 24.5% (98/400), and E. bieneusi was 13.5% (54/400). G. duodenalis, Blastocystis spp., and E. bieneusi were detected in all of the zoos, exhibiting various zoonotic genotypes or subtypes. G. duodenalis-positive samples exhibited three assemblages (D, E, and B). Blastocystis spp. subtypes (ST1, ST2, ST3, ST4, ST5, ST8, ST10, ST13, and ST14) and one unknown subtype (ST) were identified. A total of 12 genotypes of E. bieneusi were identified, including SC02, BEB6, Type IV, pigEBITS 7, Peru8, PtEb IX, D, CD9, EbpC, SCBB1, CM4, and CM7. Moreover, significant differences in the positive rates among different zoos were observed (p < 0.01). The findings indicate that zoo animals in China are affected by a range of intestinal protozoa infections. Emphasizing molecular identification for specific parasite species or genotypes is crucial for a better understanding of the zoonotic risk. Preventing and controlling parasitic diseases in zoos is not only vital for zoo protection and management but also holds significant public health implications.

show abstract

Section: Discussionmentioning

confidence: 99%

Epidemiology and Molecular Characterization of Zoonotic Gastrointestinal Protozoal Infection in Zoo Animals in China

An,

Jiang,

Zhang

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

“…There has been one report of assemblage C in eastern gray kangaroos (Ng et al 2011 ) and two reports of assemblage D in eastern gray kangaroos (Ng et al 2011 ) and a single quenda (Hillman et al 2019 ). Assemblage E has been reported in a single quenda (Thompson et al 2010 ), eastern gray kangaroo (Zahedi et al 2020 ), and a red-necked wallaby (Zou et al 2022 ). There has also been one report of an assemblage C and D mixture in three quendas (Thompson et al 2010 ).…”

Section: Zoonotic G Duodenalis Assemblages In Mars...mentioning

confidence: 98%

Zoonotic Cryptosporidium and Giardia in marsupials—an update

Barbosa,

Egan,

Feng

et al. 2024

Parasitol Res

View full text Add to dashboard Cite

Marsupials, inhabiting diverse ecosystems, including urban and peri-urban regions in Australasia and the Americas, intersect with human activities, leading to zoonotic spill-over and anthroponotic spill-back of pathogens, including Cryptosporidium and Giardia. This review assesses the current knowledge on the diversity of Cryptosporidium and Giardia species in marsupials, focusing on the potential zoonotic risks. Cryptosporidium fayeri and C. macropodum are the dominant species in marsupials, while in possums, the host-specific possum genotype dominates. Of these three species/genotypes, only C. fayeri has been identified in two humans and the zoonotic risk is considered low. Generally, oocyst shedding in marsupials is low, further supporting a low transmission risk. However, there is some evidence of spill-back of C. hominis into kangaroo populations, which requires continued monitoring. Although C. hominis does not appear to be established in small marsupials like possums, comprehensive screening and analysis are essential for a better understanding of the prevalence and potential establishment of zoonotic Cryptosporidium species in small marsupials. Both host-specific and zoonotic Giardia species have been identified in marsupials. The dominance of zoonotic G. duodenalis assemblages A and B in marsupials may result from spill-back from livestock and humans and it is not yet understood if these are transient or established infections. Future studies using multilocus typing tools and whole-genome sequencing are required for a better understanding of the zoonotic risk from Giardia infections in marsupials. Moreover, much more extensive screening of a wider range of marsupial species, particularly in peri-urban areas, is required to provide a clearer understanding of the zoonotic risk of Cryptosporidium and Giardia in marsupials.

show abstract

“…Assemblage D ( n = 33) was only reported in chinchillas in one study in Romania [ 39 ]. Assemblage E ( n = 11) was reported in mice ( Pseudomys albocinereus ) in Australia [ 40 ], chinchillas ( Chinchilla lanigera ) in Romania [ 39 ], and Himalayan marmots ( Marmota himalayana ) and maras ( Dolichotis patagonum ) in China [ 41 , 42 ]. The mixed of assemblage C and F was identified in wild bush rats ( Rattus fuscipes ) in Australia [ 40 ].…”

Section: Molecular Characteristics Of Giardia In R...mentioning

confidence: 99%

Role of rodents in the zoonotic transmission of giardiasis

Qin

et al. 2023

One Health

View full text Add to dashboard Cite

Prevalence and multilocus genotyping of Giardia duodenalis in zoo animals in three cities in China

Cited by 7 publications

References 50 publications

Epidemiology and Molecular Characterization of Zoonotic Gastrointestinal Protozoal Infection in Zoo Animals in China

Epidemiology and Molecular Characterization of Zoonotic Gastrointestinal Protozoal Infection in Zoo Animals in China

Zoonotic Cryptosporidium and Giardia in marsupials—an update

Role of rodents in the zoonotic transmission of giardiasis

Contact Info

Product

Resources

About