Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt

Abstract

A total of 200 random samples of meat products represented by minced meat, beef burger, beef kofta and beef sausage (50 of each) were collected from different supermarkets at El Menofiya and Cairo Governorates. The collected samples were examined for isolation of Psuedomonas and molecular characterization by using Multiplex PCR. psuedomonas was isolated from examined samples with the percentage of 18% (9) ,30% (15), 26% (13) and 22% (11) in minced meat, beef burger, beef kofta and beef sausage, respectively. The isolated pseudomonas could be serologically identified as P. aeruginosa ,P. diminuta ,P. fluorescens , P. proteolytica, P. fragi , P. putrefaciens and P. putida. Multiplex PCR results showed that the prevalence of virulence genes of P. aeruginosa were exotoxin A gene (ETA) 36%(4), gryase B gene (gyrB) with outer membrane protein gene (oprL) 54% (6), exotoxin A gene (ETA) with outer membrane protein gene (oprL) 18% (2) and gryase B gene (gryB) with exotoxin A gene (ETA) with outer membrane protein gene (oprL) (18%(2). The prevalence of Pseudomonas species in meat products samples is highly significant Therefore, its presence should be avoided during first stages of food processing.