Prevalence and persistence of foreign DNA beneath fingernails

Matte, Melinda; Williams, Linda Ruth; Frappier, Roger; Newman, Jonathan

doi:10.1016/j.fsigen.2011.05.008

Cited by 37 publications

(28 citation statements)

References 8 publications

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“…This non-self DNA was possibly present on the hands prior to experiment commencement or alternatively picked-up during the experiment from the ''dirty'' objects/surfaces. This is supported by a number of studies which show that hands can contain non-self DNA [19][20][21][22][23][24][25][26]. An example of this was the multi-step transfer of DNA in experiment 1 where DNA from the chair arm was picked up by a participant's hand and transferred to other experimental surfaces.…”

Section: Discussionmentioning

confidence: 90%

The complexities of DNA transfer during a social setting

Goray

Oorschot

2015

Legal Medicine

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Section: Discussionmentioning

confidence: 90%

The complexities of DNA transfer during a social setting

Goray

Oorschot

2015

Legal Medicine

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“…In such cases where physical contact between the victim and the assailant had occurred or was a possibility, a prevalence of almost 30% was reported [3,4]. However, this estimator decreased to almost 5% when samples were collected from individuals deceased of non-violent death [6].…”

Section: Discussionmentioning

confidence: 99%

“…In the present review foreign DNA was detected in 9.8% of the cases analyzed, a value which ranges in between both case groups. Even though, the presence of foreign DNA under the fingernails may be important for proving any sort of contact between individuals, the expert testimony should be enough clear with regard to The detection of background levels of foreign DNA beneath the fingernails of volunteer donors has also been reported, with variable results depending on the type of contact [3,4]. Even though, the presence of foreign DNA under the fingernails may be important for proving any sort of contact between individuals, the expert testimony should be clear in relation to the reaches and limitations of this type of evidence…”

Section: Discussionmentioning

confidence: 99%

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Analysis of DNA from fingernail samples in criminal cases

Bozzo¹,

Colussi²,

Ortiz³

et al. 2015

Forensic Science International: Genetics Supplement Series

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The detection of foreign DNA from the fingernail hyponychium may be relevant in the investigation of certain criminal acts, providing evidence of any sort of contact between the victim and the suspect.In this report, we analyzed results obtained from 164 fingernail samples collected by three different methods: extraction, clipping and swabbing. Samples were taken, mostly during the autopsy operation, and submitted to our Lab in the context of 71 different criminal cases.In 74.6% of the reviewed cases, a reportable DNA profile which matched the victims was recovered. In 13.2% of these profiles, foreign DNA was also detected as a minor contributor, representing almost a 10% of the analyzed cases. The estimated prevalence of foreign DNA samples beneath fingernails is comparable with that reported from volunteer donors screenings.

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“…Hansson et al (21) compared DNA collection with mini-tape vs three different swabs. There are also studies on the persistence of saliva (22), fingernails (23), biological samples (24), trace evidence (25), and the persistence of DNA in touch samples. However, no comparison has been made for the effects of four different preprocessing methods on touch DNA samples.…”

mentioning

confidence: 99%

Comparison of preprocessing methods and storage times for touch DNA samples

et al. 2017

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AimTo select appropriate preprocessing methods for different substrates by comparing the effects of four different preprocessing methods on touch DNA samples and to determine the effect of various storage times on the results of touch DNA sample analysis.MethodHand touch DNA samples were used to investigate the detection and inspection results of DNA on different substrates. Four preprocessing methods, including the direct cutting method, stubbing procedure, double swab technique, and vacuum cleaner method, were used in this study. DNA was extracted from mock samples with four different preprocessing methods. The best preprocess protocol determined from the study was further used to compare performance after various storage times. DNA extracted from all samples was quantified and amplified using standard procedures.ResultsThe amounts of DNA and the number of alleles detected on the porous substrates were greater than those on the non-porous substrates. The performances of the four preprocessing methods varied with different substrates. The direct cutting method displayed advantages for porous substrates, and the vacuum cleaner method was advantageous for non-porous substrates. No significant degradation trend was observed as the storage times increased.ConclusionDifferent substrates require the use of different preprocessing method in order to obtain the highest DNA amount and allele number from touch DNA samples. This study provides a theoretical basis for explorations of touch DNA samples and may be used as a reference when dealing with touch DNA samples in case work.

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Prevalence and persistence of foreign DNA beneath fingernails

Cited by 37 publications

References 8 publications

The complexities of DNA transfer during a social setting

The complexities of DNA transfer during a social setting

Analysis of DNA from fingernail samples in criminal cases

Comparison of preprocessing methods and storage times for touch DNA samples

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