Prevalence and phylogenetic characterization of Listeria monocytogenes isolated from processed meat marketed in Egypt

Mohamed, Y. A.; Reda, Waffa W; Abdel-Moein, Khaled A.; El-Razik, Khaled A. Abd; Barakat, Ashraf M.; Fadaly, Hassan A. El; Hassanain, Nawal A.; Hegazı, Ahmed G.

doi:10.1016/j.jgeb.2016.06.001

Cited by 11 publications

(6 citation statements)

References 21 publications

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“…Although other studies reported a lower prevalence of L. monocytogenes. For example, bacteria were detected in 5.3% of meat products in China (24), 4% in Egypt (25) and 0.7% in Estonia (26). In the current study, the prevalence of L. monocytogenes in roasted fish, roasted chicken meat and cocked beef were similar to that in Shi et al study (23); in which, L. monocytogenes was recovered from 6.9% of Chinese roast chicken meats and 6.5% of Chinese cocked beef.…”

Section: Discussionsupporting

confidence: 80%

Prevalence and Antibiotic Susceptibility of Listeria Monocytogenes Isolated from Retail Ready-to-Eat Meat Products in Gorgan, Iran

et al. 2020

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Background and Objectives: Listeria monocytogenes are known as an emerged foodborne pathogen and considered as a severe health risk. In the present study, prevalence and antibiotic resistance of L. monocytogenes isolated from readyto-eat meat products in Iran were assessed. Materials and Methods: A total of 200 ready-to-eat meat products, including chicken meat (wing, breast, and leg), lamb and fish, were collected, and L. monocytogenes was isolated according to ISO 11290-1. All of the isolates were verified using polymerase chain reaction and serotyping methods. Antibiotics susceptibility of L. monocytogenes isolates was assessed using the broth microdilution method. Results: The presence of L. monocytogenes was verified in 13% of the samples. The presence of L. monocytogenes was reported in 26% of roast chicken meat, 5% of roast fish and 8.33% of cooked beef. Serology showed that Serotype 1/2a (48.13%) was the dominant serotype, followed by 4b (38.4%), 1/2c (6.99%) and 1/2b (6.48%). The result showed that 37 out of 100 L. monocytogenes isolates were resistant to all tested antibiotics. Furthermore, eight isolates were intermediately multi-resistant to the antibiotics. The rate of antibiotic resistance in L. monocytogenes was 52% in Serotype 1/2a, 39% in Serotype 4b, and 35% in Serotype 1/2c. Isolates of L. monocytogenes were mostly resistant to penicillin, ampicillin and erythromycin, but highly susceptible to tetracycline and gentamycin. Conclusions: The high level of L. monocytogenes prevalence and its resistance to antibacterial agents in meat products may result in severe human listeriosis. Therefore, it is necessary to use efficient monitoring protocols for antibiotic administration and further safety management systems in food production units.

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Section: Discussionsupporting

confidence: 80%

Prevalence and Antibiotic Susceptibility of Listeria Monocytogenes Isolated from Retail Ready-to-Eat Meat Products in Gorgan, Iran

et al. 2020

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“…In this study, the diagnosis of L. monocytogenes isolates from clinical and food samples was confirmed using PCR, identified 13 L. monocytogenes isolates sheltering hly A gene, which agree with other previous studies such as [18] in Egypt who mentioned that all L. monocytogenes isolates are positive for hlyA gene from meat. Furthermore, Kamar et al [19], in Egypt, detected hlyA gene in all L. monocytogenes isolated from different sources.…”

Section: Discussionsupporting

confidence: 90%

Phylogenetic Charaterization of Listeria Monocytogenes Isolated From Different Sources in Iraq

Yousif

AL-Shamari

2018

Asian J Pharm Clin Res

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Objective: The main goal of the current study was to isolate and detect the phylogenetic characterization of Listeria monocytogenes isolate from clinical and non-clinical specimens.Methods: L. monocytogenes was isolated from 353 samples including: (94) Vaginal swabs, (81) piece of placenta, (51) frozen chicken, (69) soft cheese, and (58) frozen red meat samples using the Association of Official Agricultural Chemists method. Polymerase chain reaction (PCR) was performed for the detection of virulence gene hlyA followed by DNA sequence analysis for this gene.Results: A total of 13 isolates of L. monocytogenes were isolated from 2 (2.1%) vaginal swabs, 4 (4.9%) piece placenta, 2 (3.9%) frozen chicken, 3 (4.3%) frozen soft cheese, and 2 (3.4%) frozen red meat samples, and hlyA gene was detected in 100% of L. monocytogenes isolates.Conclusion: All the isolates tested positive for hlyA gene, so this is important role in the study of the L. monocytogenes infection and its pathogenicity. The phylogenetic analysis showed a clear convergence in the L. monocytogenes isolates from chicken, red meat, and soft cheese 70%, while there is a marked difference in isolates of L. monocytogenes isolated from placenta and vaginal swabs.

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“…The minced meat samples were totally negative in all the three companies (table 1). These results were agreed with (Mohamed et al, 2016) who found that L. monocytogenes were confirmed distributed in beef burgers samples (4%), minced meat (4%) and Luncheon (4%).…”

Section: Morphological Characterization Of Bacteria On Specific Mediusupporting

confidence: 92%

Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

Hassan

Abdal-Aziz

Megahed

et al. 2019

Alexandria Science Exchange Journal

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Three kinds of processed meat (Luncheon, Burger , Minced meat) were examined for the presence of Listeria species. Collected samples purchased from local supermarkets were from three different Egyptian companies. All the bacterial isolates were cultured on specific media after pre-enrichment and enrichment cultures. The colonial morphology depends strongly up on the media used and the incubation conditions provided. (Oxford Listeria Agar Base) was used as specific medium for Listeria. The bacterial distribution of Listeria was, 2.22% in Beef burger, 13.6%. in luncheon and there was no bacterial growth in minced meat. In this study; gram staining., morphological characterization, molecular identification using 16S rDNA partial sequencing and specific gene Iap gene of Listeria were carried out.

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Prevalence and phylogenetic characterization of Listeria monocytogenes isolated from processed meat marketed in Egypt

Cited by 11 publications

References 21 publications

Prevalence and Antibiotic Susceptibility of Listeria Monocytogenes Isolated from Retail Ready-to-Eat Meat Products in Gorgan, Iran

Prevalence and Antibiotic Susceptibility of Listeria Monocytogenes Isolated from Retail Ready-to-Eat Meat Products in Gorgan, Iran

Phylogenetic Charaterization of Listeria Monocytogenes Isolated From Different Sources in Iraq

Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

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