Prevalence and risk analysis of mobile colistin resistance and extended-spectrum <i>β</i>-lactamase genes carriage in pet dogs and their owners: a population based cross-sectional study

Lei, Lei; Wang, Yongqiang; He, Jiakang; Cai, Chang; Liu, Qingzhi; Yang, Dengfeng; Zou, Zhiyu; Shi, Lingyu; Jia, Jianqin; Wang, Yang; Walsh, Timothy R.; Shen, Jianzhong; Zhong, Yougang

doi:10.1080/22221751.2021.1882884

Cited by 21 publications

(28 citation statements)

References 35 publications

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“…The close contact between companion animals and humans enhances the risk of bacterial and even antimicrobial-resistant bacterial transmission across animals or humans by horizontal transfer and clonal spread [ 11 ]. Previous studies have shown that antimicrobial-resistant E. coli are frequently isolated from dogs and cats [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Companion Animals as Potential Reservoirs of Antibiotic Resistant Diarrheagenic Escherichia coli in Shandong, China

Cui

Zhao

et al. 2022

Antibiotics

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Antibiotic resistance genes of Escherichia coli (E. coli) from companion animals were still poorly understood. Here, we investigated the extended-spectrum β-lactamases (ESBLs) resistance genes of E. coli from companion animals in Shandong, China. A total of 79 isolates (80.6%) were recovered from 98 healthy or diarrheal companion animals in 2021, among which ESBLs-producing isolates accounted for 43.0% (34/79), and more than half of ESBL E. coli (ESBL-EC) strains (n = 19) were isolated from healthy companion animals. Diarrheagenic E. coli isolates (45.6%, n = 36) were represented by enterotoxigenic (ETEC) (32.9%), enteropathogenic (EPEC) (10.1%) and enteroinvasive (EIEC) (2.6%), 20 isolates of which were from healthy pets. Among tested antibiotics, resistance to tetracycline (64.6%) was the most commonly observed, followed by doxycycline (59.5%) and ampicillin (53.2%). Notably, all isolates were susceptible to meropenem. The multidrug-resistant (MDR) rate was 49.4%, 20 isolates of which were ESBLs producers; moreover, 23.4%, 16.4% of ESBL-EC strains were resistant to 5 or more, 7 or more antibiotics, respectively. Among the 5 β-lactamase resistance genes, the most frequent gene was blaCTX-M (60.76%), followed by blaSHV (40.51%). The plasmid-mediated quinolone resistance (PMQR) gene aac(6’)-Ib-cr was detected in 35 isolates. Additionally, ESBL-associated genes (i.e., blaCTX-M, blaSHV) were found in 76.5% ESBL-EC strains, with six isolates carrying blaCTX-M and blaSHV. The marker gene of high-pathogenicity island gene irp2 (encoding iron capture systems) was the most frequency virulence gene. Our results showed that ESBL-EC were widespread in healthy or diarrhea companion animals, especially healthy pets, which may be a potential reservoir of antibiotic resistance, therefore, enhancing a risk to public and animal health.

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Section: Introductionmentioning

confidence: 99%

Companion Animals as Potential Reservoirs of Antibiotic Resistant Diarrheagenic Escherichia coli in Shandong, China

Cui

Zhao

et al. 2022

Antibiotics

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“…mcr genes were detected in E. coli and Pseudomonas aeruginosa [ 41 ]. However, dogs and their owners have high potential colonization of antibiotic-resistant bacteria [ 42 ]. Colistin-resistance genes have also recently been detected in dogs in South Korea [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

“…Zoonotic transmission from pets to their owners has already been reported in several studies. It is important to establish strict hygiene conditions and diverse approaches to reduce zoonotic transmission of mcr -producing bacteria [ 42 , 47 , 48 ]. Bacteria that carry mcr genes can cause diseases such as urinary tract infections, previously reported to be linked to the presence of E. coli carrying the mcr-1 gene [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Screening of Colistin-Resistant Bacteria in Domestic Pets from France

Hamame

Davoust

Rolain

et al. 2022

Animals

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Background: Pets are the closest animals to humans with a considerable risk of zoonotic transmission. This study aimed to screen colistin-resistant bacteria from stools of dogs and cats from Marseille, France. Screening of mcr genes in pets has never been reported in France. Methods: Fecal samples (n = 157) were cultivated on the selective Lucie-Bardet Jean-Marc-Rolain medium (LBJMR). Bacteria were identified using Microflex LS MALDI-TOF. The antibiotic resistance phenotype was investigated for several antibiotics (β-lactams, aminoside, cephalosporine, tetracycline, and sulfonamide). PCR techniques were performed to detect mcr genes. Results: A total of 218 bacteria were identified. For cats, intrinsically colistin-resistant bacteria were significantly higher than mcr-1 gene carriers (n = 4). Dogs had more bacteria with the mcr-1 gene (n = 10). Furthermore, cats had a high prevalence of Gram-positive bacteria (GPB), whereas dogs had GNB equal to GPB. The diversity of identified bacteria was due to the constitution of the pets’ microorganisms. Even though colistin use is monitored in France, pets harbor various colistin-resistant bacteria. Additionally, in this geographical area, bacteria bearing mcr-1 gene from dogs and cats were detected for the first time. Conclusions: The current study opens a new perspective: the spread of colistin resistance is independent of colistin use. What are the most factors related to the emergence of colistin resistance? The surveillance of pets must be considered a priority to avoid the spread of mcr genes. It is important to know the contribution that pets make to the pool of multidrug-resistant mcr-1-containing bacteria.

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“…However, in the case of companion animals, such as dogs and cats, not only veterinarians but also animal owners are at risk of acquiring multiresistant zoonotic bacterial pathogens. Previous studies have shown that there are numerous examples of dog and cat owners sharing (multi)resistant bacteria with their pets [ 8 , 9 , 10 , 11 ]. Enterococci, in particular Enterococcus faecalis and Enterococcus faecium , but also the Gram-negative species Escherichia coli , Pseudomonas aeruginosa , and Acinetobacter baumannii have zoonotic potential.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Biocide Resistance among Canine and Feline Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii Isolates from Diagnostic Submissions

et al. 2022

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A total of 215 isolates from infections of dogs and cats, including 49 Enterococcus faecalis, 37 Enterococcus faecium, 59 Escherichia coli, 56 Pseudomonas aeruginosa, and 14 Acinetobacter baumannii, were investigated for their susceptibility to 27 (Gram-positive bacteria) or 20 (Gram-negative bacteria) antimicrobial agents/combinations of antimicrobial agents by broth microdilution according to the recommendations of the Clinical and Laboratory Standards Institute. Moreover, all isolates were analysed for their susceptibility to the biocides benzalkonium chloride, chlorhexidine, polyhexanide, and octenidine by a recently published broth microdilution biocide susceptibility testing method. While the E. faecalis isolates did not show expanded resistances, considerable numbers of the E. faecium isolates were resistant to penicillins, macrolides, tetracyclines, and fluoroquinolones. Even a single vancomycin-resistant isolate that carried the vanA gene cluster was detected. Expanded multiresistance phenotypes were also detected among the E. coli isolates, including a single carbapenem-resistant, blaOXA-48-positive isolate. In addition, multiresistant A. baumannii isolates were detected. The minimal inhibitory concentrations of the biocides showed unimodal distributions but differed with respect to the biocide and the bacterial species investigated. Although there were no indications of a development of biocide resistance, some P. aeruginosa isolates exhibited benzalkonium MICs higher than the highest test concentration.

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Prevalence and risk analysis of mobile colistin resistance and extended-spectrum β-lactamase genes carriage in pet dogs and their owners: a population based cross-sectional study

Abstract: Prevalence and risk analysis of mobile colistin resistance and extended-spectrum βlactamase genes carriage in pet dogs and their owners: a population based cross-sectional study, Emerging Microbes & Infections,

Cited by 21 publications

References 35 publications

Companion Animals as Potential Reservoirs of Antibiotic Resistant Diarrheagenic Escherichia coli in Shandong, China

Companion Animals as Potential Reservoirs of Antibiotic Resistant Diarrheagenic Escherichia coli in Shandong, China

Screening of Colistin-Resistant Bacteria in Domestic Pets from France

Antimicrobial and Biocide Resistance among Canine and Feline Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii Isolates from Diagnostic Submissions

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