Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals

Beutin, Lothar; Geier, D; Steinrück, Hartmut; Zimmermann, Sabine; Scheutz, Flemming

doi:10.1128/jcm.31.9.2483-2488.1993

Cited by 484 publications

(308 citation statements)

References 33 publications

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“…These false positives may occur when E. coli isolates produce a narrow and turbid halo because of a low expression of alpha-haemolysin or when vancomycin enhances the haemolysis of some non-STEC which may show weak haemolysis [3]. It is also possible that the faecal multiplex PCR primers [7] may not have detected some haemolysin variants [4] or the mEC broth may have had an inhibitory e¡ect on the PCR assay. A disadvantage of the direct culture on BVCCA for the detection of complex STEC is that it does not select for eaeA positive and ehxA negative STEC.…”

Section: Discussionmentioning

confidence: 99%

The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR

Hornitzky¹

2001

FEMS Microbiology Letters

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The prevalence of complex Shiga toxin-producing Escherichia coli (STEC), i.e. STEC containing accessory virulence factors intimin (eaeA) and/or enterohaemorrhagic E. coli haemolysin (ehxA) and their serotypes were determined in diagnostic bovine faecal samples processed during a 3 months period. The presence of complex STEC was determined using PCR and vancomycin-cefixime-cefsulodin blood agar (BVCCA) using a dual approach which involved (i) direct culture of faecal samples on BVCCA followed by mutiplex PCR of BVCCA positive colonies and (ii) culture of faecal samples enriched in modified EC (mEC) broth (with a complex STEC profile determined by PCR) on BVCCA followed by multiplex PCR of BVCCA positive colonies. Using both techniques complex STEC were isolated from 23 (18.7%) of the 123 faecal samples. Complex STEC were isolated from 14 faecal samples by direct culture on BVCCA and 13 faecal samples yielded complex STEC by culture of mEC broths with a complex STEC profile on BVCCA. Only four samples were positive using both techniques. The serotypes isolated included O5:H-, O26:H-, O26:H11, O91:H21, O111:H-, O111:H8, O104:H11, O113:H21 and O157:H8. This study confirms that non-O157 STEC can be isolated from bovine faeces and that they carry types associated with human disease. This work also demonstrates that the use of a dual approach is advisable to increase the likelihood of isolating complex STEC. ß 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Section: Discussionmentioning

confidence: 99%

The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR

Hornitzky¹

2001

FEMS Microbiology Letters

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“…Studies performed in Sweden on cats either with or without symptoms of diarrhea established a 40% prevalence for STEC in clinically healthy cats and 95% in cats with diarrhea (Abaas et al, 1989). Other studies in Germany have reported a prevalence of around 4% and 14% in healthy and symptomatic pets, respectively (Beutin et al, 1993). The isolation of STEC O157:H7 in a dog (Khakhria et al, 1990) and a clinical case associated with HUS symptoms in a dog (Dell'Orco et al, 2005) have also been reported, although in the latter it is not clear whether the strain isolated was actually STEC.…”

Section: Introductionmentioning

confidence: 97%

Shiga toxin-producing and attaching and effacingEscherichia coliin cats and dogs in a high hemolytic uremic syndrome incidence region in Argentina

Bentancor¹,

Rumi²,

Gentilini³

et al. 2007

FEMS Microbiology Letters

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Shiga toxin-producing Escherichia coli (STEC), responsible for the hemolytic uremic syndrome, is an endemic pathogen in Argentina. We studied the prevalence of STEC in fecal samples from cats and dogs of Buenos Aires city and suburbs. Cultures were used for screening stx1/stx2 and rfbO157 by multiplex PCR. All E. coli-positive colonies for these genes were further characterized for the eae gene and for serotypes. In dogs, 17 (3.7%), 19 (4.2%) and 34 (7.5%) of samples were positive for stx2, stx1 and rfb, respectively. In cats, six (4.0%) of the samples were positive for stx2, three (2.0%) for stx1 and four (2.7%) for rfbO157. In 18 (4.0%) of the dog samples, a bacteriological diagnosis was obtained by isolation. The percentage of positive isolates corresponding to the rfbO157 and to the stx2 genotypes were 2.9% and 1.1%, respectively. In four of the cat samples, the bacteriological diagnosis for stx2 (2.6% prevalence of STEC) was confirmed. Although these data suggest that the high infection index of STEC in children in Argentina does not seem to be due mainly to the role of cats and dogs, there are some strains with virulence genes in common for humans and their domestic animals.

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“…Escherichia coli O157:H7: an emerging food-and water-borne pathogen detected in a wide spectrum of animal species (cattle, sheep, goat, deer, moose, swine, horse, dog, cat, pigeon, chicken, turkey, gull) sometimes even with considerable prevalence (Beutin et al, 1993;Wieler et al, 1996). In particular, cattle have been identified as major reservoirs of STEC strains that are highly virulent in the human host (e.g.…”

Section: Introductionmentioning

confidence: 99%

The ecological habitat and transmission ofEscherichia coliO157:H7

Chekabab

Paquin-Veillette

Dozois

et al. 2013

FEMS Microbiol Lett

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Since its first description in 1982, the zoonotic life-threatening Shiga toxin-producing Escherichia coli O157:H7 has emerged as an important food- and water-borne pathogen that causes diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans. In the last decade, increases in E. coli O157:H7 outbreaks were associated with environmental contamination in water and through fresh produce such as green leaves or vegetables. Both intrinsic (genetic adaptation) and extrinsic factors may contribute and help E. coli O157:H7 to survive in adverse environments. This makes it even more difficult to detect and monitor food and water safety for public health surveillance. E. coli O157:H7 has evolved in behaviors and strategies to persist in the environment.

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Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals

Cited by 484 publications

References 33 publications

The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR

The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR

Shiga toxin-producing and attaching and effacingEscherichia coliin cats and dogs in a high hemolytic uremic syndrome incidence region in Argentina

The ecological habitat and transmission ofEscherichia coliO157:H7

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