Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Abu, Amanee; Sutthikornchai, Chantira; Mahittikorn, Aongart; Koompapong, Khuanchai; Chiabchalard, Rachatawan; Soonthornworasiri, Ngamphol; Popruk, Supaluk

doi:10.3390/ijerph20010204

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…In several rural villages, ST3 was the most prevalent subtype [ 32 , 33 ]. Previous reports have demonstrated that the prevalence of ST3 is very common among children [ 34 , 35 ]. Even though numerous investigations have been carried out, it is still unclear if a specific subtype is related to a particular clinical characteristic.…”

Section: Introductionmentioning

confidence: 99%

Systematic Review and Meta-Analysis: Epidemiology of Human Blastocystis spp. Infection in Malaysia

et al. 2023

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Blastocystis spp. is a unicellular enteric protozoan parasite in humans with a controversial role in disease etiology. It is common in developing countries among immunocompromised patients and people who have close contact with animals. In this study, we have systematically reviewed previous studies on the distribution and genotypes of human Blastocystis infection in Peninsular Malaysia. Studies examining the prevalence of Blastocystis in diverse demographics, including rural, urban, comorbid conditions, and high-risk populations, were taken into consideration. The infection has been reported in nine states; the total percentage of infection was 17.8% (1671/9397), with the most cases in Pahang (27.3%) and the least in Johor (3.4%). Molecular studies revealed the presence of six subtypes: ST1, ST2, ST3, ST4, ST5, and ST6. ST3 was reported as the predominant subtype in all the states, with a prevalence of 54.7% (338/618). The findings provide greater clarity on the epidemiology of Blastocystis in Malaysia, which will help in policy making towards planning and strategizing control measures against the parasite.

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Section: Introductionmentioning

confidence: 99%

Systematic Review and Meta-Analysis: Epidemiology of Human Blastocystis spp. Infection in Malaysia

et al. 2023

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“…ST2, ST3, and ST1 were the most common subtypes, respectively (Güreser et al 2022). In a study that determined the prevalence and subtypes distribution of Blastocystis among school-aged children in Thailand, the most common subtypes were ST3, followed by ST1 and ST2 (Abu et al 2022). In Spain, Blastocystis sp.…”

Section: Discussionmentioning

confidence: 99%

Investigation of intestinal parasites by conventional and molecular methods in children with gastrointestinal system complaints

Özkan-Ahmetoğlu¹,

Demirel

Taşar³

et al. 2023

Parasitol Res

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Background and aims:Intestinal parasitic infections are a global health problem that causes morbidity and mortality, especially in children living in rural areas. In this study, stool samples of pediatric patients with gastrointestinal complaints were examined by conventional and molecular methods to determine the prevalence of intestinal parasites. Methods:A total of 100 pediatric patients with gastrointestinal complaints and 50 healthy children were included in the study. Stool samples were collected from each child and examined by direct microscopic examination (native-Lugol method), formol-ethyl acetate concentration technique, Kinyoun's acid-fast staining, and Wheatley trichrome staining methods. Real-time PCR was used for the detection of Blastocystis sp. and D. fragilisin the stool samples. Sanger sequencing was used to identify Blastocystissp. subtypes. Results:One or more intestinal parasites were found in 12% (n=100) of the patient group and 1% (n=50) of the control group using conventional techniques. By using real-time PCR, Blastocystis sp. was discovered in 14% (14/100) of the patient group and 4% (4/50) of the control group. There was no signi cant difference in the frequency of Blastocystis sp. between the two groups. The most prevalent Blastocystissubtype was ST1 and the most frequent allele was a2 among the samples successfully ampli ed and sequenced. D. fragilis was detected in 17% (17/100) of the patient group and 8% (4/50) of the control group by real-time PCR. The prevalence of D. fragiliswas not signi cantly different between the patient and control groups, as well. Conclusions:Blastocystis sp. and D. fragilis were found in high prevalence in pediatric patients with gastrointestinal complaints in this study. Although the role of these protists as a pathogen in humans is still controversial, it is supposed to the presence of the parasites are associated with gastrointestinal disorders such as diarrhea, abdominal pain, nausea, and vomiting. More case-control studies are needed to understand the pathogenic or commensal role of these parasites on the intestinal microbiota, especially in both patients with gastrointestinal disorders and healthy individuals.

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Effect of Blastocystis sp. infection on hematological parameters and trace element status in children with iron-deficiency anemia

Hamdy,

Fahmey,

Abd El Wahab

et al. 2024

J Parasit Dis

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Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand

Cited by 6 publications

References 41 publications

Systematic Review and Meta-Analysis: Epidemiology of Human Blastocystis spp. Infection in Malaysia

Systematic Review and Meta-Analysis: Epidemiology of Human Blastocystis spp. Infection in Malaysia

Investigation of intestinal parasites by conventional and molecular methods in children with gastrointestinal system complaints

Effect of Blastocystis sp. infection on hematological parameters and trace element status in children with iron-deficiency anemia

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