Prevalence and Virulence of Commensal Pseudomonas Aeruginosa Isolates from Healthy Individuals in Southern Vietnam (2018–2020)

Abstract

Understanding the colonization of Pseudomonas aeruginosa (P. aeruginosa) in healthy humans is useful for future prevention and treatment of P. aeruginosa infection. This study aimed to investigate the prevalence and risk factors of of P. aeruginosa colonization in healthy humans. At the same time, the virulence of the isolated P. aeruginosa was also studied. In the study, 609 Vietnamese volunteers (310 females and 299 males, age range of 2 to 73 years), who had no acute infection or disease symptoms participated at the time of sample collection. Samples were taken from the throat, nostrils, and outer ears. P. aeruginosa was found in 19 participants (3.12%, 95% CI: 0.017–0.045), mainly from the throat (11/19, 57.89%). Participants with a history of sinusitis were 11.57 times more likely to be colonized with P. aeruginosa than participants without a history of sinusitis (OR: 11.57, 95% CI: 4.08–32.76, p-value < 0.0001, Fisher’s Exact test). Age and sex were not significantly associated with P. aeruginosa colonization. Among 16 P. aeruginosa isolates used in virulence tests, 100% (16/16) were positive for the synthesis of biofilm, pyocyanin, and siderophores; 93.75% (15/16) isolates were positive for the synthesis of gelatinase and protease; and 50% (8/16) isolates were positive for lipase. There were no differences in the pattern and range of virulence factors of P. aeruginosa isolates taken from participants with and without sinusitis history. P. aeruginosa colonized 3.12% of participants, and its presence was associated with sinusitis history.

Pharmacological Effects of Volatile Oil from Chrysanthemum and Its Associated Mechanisms: A Review

“…Chrysanthemum is a medicinal and edible herb containing abundant VO. Over the years, the utility of Chrysanthemum VO as a therapeutic agent with great potential has been gradually recognized by researchers in various fields, not only in clinical and cosmetic skin care as a potential natural bacteriostatic or antioxidant agent and in other therapeutic drugs [129,130] , but also as a potential natural antiseptic additive and biodegradable food packaging in the food industry [131] . The VO extract is also a natural biological insecticide that is commonly used in agricultural pest control [132] .…”

Adaptive response of Pseudomonas aeruginosa under serial ciprofloxacin exposure

“…Pyocyanin was extracted with chloroform and 0.2 M HCl and 125 µl of the red layer was transferred to a 96-well microtitre plate. The concentration of pyocyanin (μg µl -1 ) was estimated by multiplying the optical density (OD) at 520 nm by 17.072, following the previous study [16]. E. coli was used as a negative control.…”

Bioprospecting the Skin Microbiome: Advances in Therapeutics and Personal Care Products

“…Bacteriocins represent an attractive source of antioncogenic compounds arising from their specificity, nontoxicity and abundance within the skin microbiome [ 208 ]. Pyocins (bacteriocins produced by the genus Pseudomonas ) of the transient opportunistic pathogen Pseudomonas aeruginosa selectively inhibit the proliferation of human hepatoma, B lymphocyte and murine fibroblast cell lines [ 209 , 210 ]. The antioncogenic activity of bacteriocins produced by skin-relevant pathogens has been described, such as Klebsiella pneumoniae -derived microcin E492; however, the therapeutic potential of skin-microbiome-derived bacteriocins remains unexplored [ 208 ].…”