Prevalence, Antibiotic Susceptibility and Diversity of Vibrio parahaemolyticus Isolates in Seafood from South China

Abstract

Vibrio parahaemolyticus is a leading cause of foodborne infections in China and a threat to human health worldwide. The main objective of this study is to determine the prevalence and characteristic of V. parahaemolyticus isolates in fish, oyster and shrimp samples from the South China domestic consumer market. To accomplish this, we examined 504 seafood samples from 11 provinces of China. The prevalence rates were 9.38, 30.36, and 25.60%, respectively. In summer (33.33%), the prevalence of V. parahaemolyticus was more common than that detected in the winter (14.01%). In addition, we identified 98 V. parahaemolyticus strains. The antimicrobial resistance trends of our seafood isolates to 15 antimicrobial agents revealed that major isolates were resistant to ampicillin (79.59%). Furthermore, 68.38% of the isolates were identified as being multidrug resistance. The prevalence of tdh or trh genes among the isolates was 8.16 and 12.24%, respectively. ERIC-PCR and multilocus sequence typing (MLST) results enabled classification of the isolates (n = 98) into different clusters, revealing genetic variation and relatedness among the isolates. Thus, our findings demonstrate the prevalence of V. parahaemolyticus in a variety of common seafood consumed domestically in China and provides insights into the dissemination of antibiotic-resistant strains, which should improve our microbiological risk assessment knowledge associated with V. parahaemolyticus in seafoods.

Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia

“…In fact, 64% of the Vibrio isolates were resistance to at least three or more antibiotics. These findings were similar with a previous study reported that 68% of the Vibrio isolates were resistance to at least three or more antibiotics (Yang et al, 2017).…”

“…Over 80% of V. harveyi from fish in Italy showed resistant to ampicillin, amoxicillin and erythromycin (Scarano et al, 2014). The findings were also in agreement with studies done all around world and Malaysia (Ang et al, 2010;Al-Othrubi et al, 2014;Elexson et al, 2014;Saifedden et al, 2016;Tan et al, 2017). Emergence of high resistance Vibrio strains against ampicillin and penicillin was related with the extensive used of both antibiotics and could influence the disease management in aquaculture system (FAO, 2017).…”

“…in Malaysia (Han et al, 2007;Noorlis et al, 2011). In addition, many studies have proven that V. parahaemolyticus isolated from fish and other aquatic animals was susceptible to tetracycline and streptomycin (Lesmana et al, 2001;Han et al, 2007;Kumar et al, 2009;Noorlis et al, 2011;Ottaviani et al, 2013;Yano et al, 2014;Oramadike and Ogunbanwo, 2015;Tan et al, 2017).…”

“…Similarly, V. parahaemolyticus isolated from croaker fish (P. senegalensis) and blue crab (Callinectes sapidus) at Lagos Lagoon, Nigeria, showed resistance to ampicillin (Oramadike and Ogunbanwo, 2015). In China, 79.6% of V. parahaemolyticus isolated from fish, shrimp and oyster were resistant to ampicillin (Yang et al, 2017).…”

Managing the risk of Vibrio parahaemolyticus infections associated with oyster consumption: A review

“…Table 1 shows examples of studies that have investigated the presence of V. parahaemolyticus in oysters collected from either culturing environments or markets in either tropical, subtropical, or temperate areas. Oyster samples collected from tropical or subtropical areas (Cook et al., 2002; Deepanjali et al., 2005; DePaola, Nordstrom, Bowers, Wells, & Cook, 2003; Han et al., 2017; Johnson et al., 2010, 2012; López‐Hernández, Pardío‐Sedas, Lizárraga‐Partida, Williams, Martínez‐Herrera, Flores‐Primo, Uscanga‐Serrano, & Rendón‐Castro, 2015; Matté, Matté, Rivera, & Martins, 1994; New et al., 2014; Sanjeev & Stephen, 1993; Sobrinho et al., 2010, 2011; Ward & Bej, 2006; Yang et al., 2017; Yu et al., 2013, 2016; Zimmerman et al., 2007) are generally considered to exhibit higher concentrations of V. parahaemolyticus than those obtained from temperate areas (Cruz et al., 2015; Duan & Su, 2005b; Fletcher, 1985; Jones et al., 2014; Kaysner, Abeyta, Stott, Krane, & Wekell, 1990; Kaysner, Abeyta, Stott, Lilja, & Wekell, 1990; Kirs et al., 2011; Lopatek, Wieczorek, & Osek, 2015; Lopez‐Joven et al., 2015; Mok et al., 2019; Nakaguchi, 2013; Park, Mok, et al., 2018; Parveen et al., 2008; Roque et al., 2009; Ryu, Mok, Lee, Kwon, & Park, 2019; Tepedino, 1982; Thomson & Thacker, 1972). For example, concentrations of V. parahaemolyticus in oysters were 94% in India, between 77% and 100% in Brazil, 100% in Mexico, and 71% in Taiwan.…”