Prevalence, antimicrobial resistance and genetic lineages of <i>Enterococcus</i> spp. from vegetable food, soil and irrigation water in farm environments in Tunisia

Saïd, Lamjed Ben; Klibi, Naouel; Dziri, Raoudha; Borgo, Francesca; Boudabous, Abdellatif; Slama, Karim Ben; Torres, Carmén

doi:10.1002/jsfa.7264

Cited by 56 publications

(40 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibacterial‐resistant Enterococcus was found in feces of pets and camels, wild birds, irrigation water from farm environments, food vegetables, hospital environments, animal meat, and patients in Tunisia . Resistance to VAN, macrolides, aminoglycosides, β‐lactams, and TET was detected in the environment, animals and humans, with the majority of the isolates being E. faecium , followed by E. faecalis .…”

Section: Resultsmentioning

confidence: 99%

Molecular epidemiology and mechanisms of antibiotic resistance inEnterococcusspp.,Staphylococcusspp., andStreptococcusspp. in Africa: a systematic review from a One Health perspective

Sekyere

Mensah

2019

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

A systematic review of antibiotic‐resistant Gram‐positive bacteria in Africa from a One Health perspective is lacking. Here, we report result from a search for English‐language articles on the resistance mechanisms and clonality of Gram‐positive bacteria in Africa between 2007 and 2019 reported in PubMed, Web of Science, ScienceDirect, and African Journals OnLine; 172 studies from 22 different African countries were identified. Resistance genes, such as mecA, erm(B), erm(C), tet(M), tet(K), tet(L), vanB, vanA, vanC, and tet(O), were found to be common. Staphylococcus spp., Enterococcus spp., and Streptococcus spp. were the main species reported by the studies, with clones such as Staphylococcus aureus ST5 (n = 218 isolates), ST8 (n = 127 isolates), ST80 (n = 133 isolates), and ST88 (n = 117 isolates), and mobile genetic elements such as IS16 (n = 28 isolates), IS256 (n = 96), Tn916 (n = 107 isolates), and SCCmec (n = 4437 isolates) identified. SCCmec IV (n = 747 isolates) was predominant, followed by SCCmec III (n = 305 isolates), SCCmec II (n = 163 isolates), SCCmec V (n = 135 isolates), and SCCmec I (n = 79 isolates). Resistance to penicillin (n = 5926 isolates), tetracycline (n = 5300 isolates), erythromycin (n = 5151 isolates), rifampicin (n = 3823 isolates), gentamycin (n = 3494 isolates), sulfamethoxazole/trimethoprim (n = 3089 isolates), and ciprofloxacin (n = 2746 isolates) was common in most reports from 22 countries. Clonal dissemination of resistance across countries and between humans, animals, and the environment was observed. Resistance rates ranged from 1.4% to 100% for 15 of the studies; 10 were One Health–related studies. Strict infection control measures, antimicrobial stewardship, and periodic One Health epidemiological surveillance studies are needed to monitor and contain the threat of increasing antibiotic resistance in Africa.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular epidemiology and mechanisms of antibiotic resistance inEnterococcusspp.,Staphylococcusspp., andStreptococcusspp. in Africa: a systematic review from a One Health perspective

Sekyere

Mensah

2019

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

show abstract

“…This microorganism has been reported to be a source of contamination especially in vegetable food (Ben Said et al . ).…”

Section: Discussionmentioning

confidence: 97%

Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by theLactobacillus salivariusSGL 03

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Antimicrobial-resistant Enterococcus was found in faeces of pet and camel, irrigation water from farm environments, food vegetables, hospital environments, animal meat and patients in Tunisia 19,22,31,51,52,69 . High-level resistance to vancomycin, macrolides, aminoglycosides, β-lactams and tetracycline was detected in the environment, animals and humans with majority of the isolates being E. faecium, followed by E. faecalis.…”

Section: Resultsmentioning

confidence: 99%

Molecular Epidemiology and Mechanisms of Antibiotic Resistance in Gram-positive Bacteria in Africa: A Systematic Review and Meta-Analysis from a One Health Perspective

Mensah

2018

Preprint

View full text Add to dashboard Cite

15 16 17 18 19 [2]HIGHLIGHTS 20  There is substantial resistance to antibiotics among Gram-positive bacteria (GPB) in Africa 21  Multidrug-resistant (MDR) S. aureus, E. faecium, E. faecalis, S. pyogenes, and S. 22 haemolyticus of the same clones were common in humans, animals and the environment. 23  MDR clones such as S. aureus ST5 and E. faecium ST80 were found in humans, animals and 24 the environment. 25  mecA, ermB, ermC, tetM/K/L, and vanA/B/C were common in GPB, including in VRSA. 26 Meta-analysis confirmed a high mean rate of drug resistance in GPB from humans (35.68%), 27 animals (69.63%) and the environment (88.33%) (p-value= 0.0301) in Africa. 28 SCCmec, IS16, and Tn916 mobilized mecA, ermB and tetM respectively across various GPB 29 species in animals, humans, and the environment. 30  A One Health approach to studying resistance mechanisms and molecular epidemiology of 31 antibiotic-resistant GPB is warranted. 32ABSTRACT 33 The emergence and dissemination of antibiotic resistance (ABR) in bacteria are being driven by 34 antibiotics use in humans, animals and the environment, threatening global health and 35 strengthening calls for a One Health approach to contain ABR. 36 A systematic search in PubMed for English research articles reporting on ABR in Gram-positive 37 bacteria in Africa within the last ten years from 2007 to 2017 was undertaken. This finally yielded 38 76 articles that were included in this review and all statistical analysis. [3]The same ABR Gram-positive bacterial clones, resistance genes, and mobile genetic elements 40 (MGEs) were found in humans, animals and the environment. IS16 and Tn916 were highly 41 associated with erm (B) and tet(M) in E. faecium (ST18, ST80 and ST910), S. agalactiae (ST612, 42 ST616 and ST617), E. faecalis and S. pyogenes (emm18, emm42, emm76 and emm118) whilst 43 SCCmec was associated with mecA in S. aureus (ST5, ST80, ST8, and ST88) and S. haemolyticus. 44 The resistance genes, mecA, erm (B), erm(C), tet(M), tet(K), tet(L), van(B), van(A), van(C), and 45 tet(O), were found in isolates from humans, animals and the environment. An ABR rate of 39.55% 46 in Gram-positive bacteria is estimated in Africa. Meta-analysis reveal that isolates were most 47 resistant to erythromycin (≥ 2 482) (37.37%), rifampicin (≥ 2 323) (33.42%), tetracycline (≥ 2 181) 48 (40.72%), penicillin (≥ 2 127) (73.47%), sulfamethoxazole/trimethoprim (≥1 377) (45.97%), 49 ciprofloxacin (≥846) (35.37%), gentamicin (≥805) (23.87%), vancomycin (≥712) (42.24%), 50 ampicillin (≥691) (48.25%), streptomycin (≥551) (32.03%) and chloramphenicol (≥376) (11.50%) 51 (p-value <0.0001). 52There is substantial resistance to antibiotics among Gram-positive bacteria in clinical and 53 environmental settings in Africa, mediated by clonal and polyclonal expansion as well as 54 horizontal transmission of resistance genes. A One Health approach to research, surveillance, and 55 molecular epidemiology, as well as antibiotic stewardship to contain ABR in humans, animals and ...

show abstract

Prevalence, antimicrobial resistance and genetic lineages of Enterococcus spp. from vegetable food, soil and irrigation water in farm environments in Tunisia

Abstract: Food vegetables in the farm or market settings are frequently contaminated by HLR-G enterococci, and these microorganisms could reach the human intestine through the food chain, if hygienic conditions are not followed. © 2015 Society of Chemical Industry.

Cited by 56 publications

References 34 publications

Molecular epidemiology and mechanisms of antibiotic resistance inEnterococcusspp.,Staphylococcusspp., andStreptococcusspp. in Africa: a systematic review from a One Health perspective

Molecular epidemiology and mechanisms of antibiotic resistance inEnterococcusspp.,Staphylococcusspp., andStreptococcusspp. in Africa: a systematic review from a One Health perspective

Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by theLactobacillus salivariusSGL 03

Molecular Epidemiology and Mechanisms of Antibiotic Resistance in Gram-positive Bacteria in Africa: A Systematic Review and Meta-Analysis from a One Health Perspective

Contact Info

Product

Resources

About