Prevalence, Antimicrobial Resistance, and Molecular Characterization of
            <i>Campylobacter</i>
            Isolated from Broilers and Broiler Meat Raised without Antibiotics

Sukumaran, Anuraj T.; Zhang, Li

doi:10.1128/spectrum.00251-22

Cited by 27 publications

(23 citation statements)

References 49 publications

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“…Thus, additional studies should be conducted to explore more in‐depth the relationship between the pathogenic traits and the antimicrobial resistance in Campylobacter strains. The high distribution of virulence factors was also reported in surveys conducted in the United States (Poudel et al., 2022 ), Poland (Wieczorek et al., 2018 ), Brazil (Takeuchi et al., 2022 ), Pakistan (Melo et al., 2013 ) and China (Zhang et al., 2016 ). We found a higher distribution of virulence factors among the C. jejuni isolates than C. coli bacteria.…”

Section: Discussionsupporting

confidence: 55%

Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

Hadiyan

Momtaz

Shakerian

2022

Veterinary Medicine & Sci

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Background Campylobacter jejuni and Campylobacter coli are the most significant Campylobacter species responsible for severe gastrointestinal disorders. Raw poultry meat is considered a source of Campylobacter transmission to the human population. Objectives The present study was aimed to assess the prevalence rate, antibiotic resistance properties, virulence characters and molecular typing of C. jejuni and C. coli strains isolated from raw poultry meat samples. Methods Three hundred and eighty raw poultry meat samples were collected and analysed for the presence of Campylobacter spp. using the microbial culture. Species identification was done using the Polymerase Chain Reaction. Disk diffusion was developed to assess the antimicrobial resistance pattern of isolates. The distribution of virulence and antimicrobial resistance genes was determined by PCR. Enterobacterial Repetitive Intergenic Consensus‐PCR was used for molecular typing. Results Campylobacter species were isolated from 6.25% of examined samples. C. jejuni and C. coli contamination rates were found to be 57.44% and 48.14%, respectively. C. jejuni strains harboured the highest resistance rate against serythromycin (42.59%), ampicillin (38.88%), ciprofloxacin (33.33%), chloramphenicol (31.48%) and tetracycline (31.48%). C. coli isolates harboured the highest resistance rate against ampicillin (73.07%), ciprofloxacin (73.07%), erythromycin (65.38%) and chloramphenicol (50%). AadE1 (44.44%), blaOXA‐61 (42.59%) and tet(O) (35.18%) were the most commonly detected resistance genes in C. jejuni and cmeB (34.61%) and blaOXA‐61 (34.61%) were the most commonly detected among C. coli strains. The most frequent virulence factors among the C. jejuni isolates were flaA (100%), ciaB (100%), racR (83.33%), dnaJ (81.48%), cdtB (81.48%), cdtC (79.62%) and cadF (74.07%). The most frequent virulence factors among the C. coli isolates were flaA (100%), ciaB (100%), pldA (65.38%) and cadF (61.53%). Conclusions The majority of C. jejuni and C. coli strains had more than 80% similarities in their ERIC‐PCR pattern, which may show their common source of transmission. The role of goose and quebec meat samples as reservoirs of virulent and antimicrobial resistant Campylobacter spp. was determined.

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Section: Discussionsupporting

confidence: 55%

Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

Hadiyan

Momtaz

Shakerian

2022

Veterinary Medicine & Sci

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“…In India, resistance to CIP and ERY reaches values of 33.3 and 21.4% in strains isolated from chicken carcasses [ 31 ]. However, Poudel et al (2022), in China, demonstrated a reduction in the levels of resistance to CIP (from 28% to 15.3%) in isolates from chickens raised without the use of antimicrobials in veterinary practices [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Resistance Challenged with Platinum(II) and Palladium(II) Complexes Containing 1,10-Phenanthroline and 5-Amino-1,3,4-Thiadiazole-2(3H)-Thione in Campylobacter jejuni

et al. 2022

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This work describes the synthesis and characterization of two metal complexes of the type [M(L1)2(phen)], where M = Pt2+ (complex I) or Pd2+ (complex II), L1 = 5-amino-1,3,4-thiadiazole-2(3H)-thiolate and phen = 1,10-phenanthroline. The in vitro antibacterial activity of these complexes was investigated in isolation and synergistically with ciprofloxacin (CIP) and erythromycin (ERY) in three strains of Campylobacter jejuni (MIC = 32 mg/L for CIP and ERY), selected from a bank of 235 strains representative of three poultry exporting states of the country (A, B and C), previously analyzed for epidemiology and resistance to CIP and ERY. A total of 53/235 (22.55%) strains showed co-resistance to CIP and ERY. Isolated resistance to CIP was higher than to ERY. Epidemiological analysis showed that resistance to CIP was more evident in state B (p < 0.0001), as well as a higher susceptibility to ERY in state C (p = 0.0028). Co-resistance was expressive in state A and in the spring and fall seasons. The evaluation of I alone and in synergy with CIP and ERY found values up to 0.25 mg/L not significant for ERY. Complex II did not show an antimicrobial effect on the three strains of tested C. jejuni. The effect provided by complex I represents a promising alternative for control of resistant strains of C. jejuni.

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“…In total, two endogenous or “housekeeping” genes, plus 20 target genes associated with immune response and D 3 -related activity were identified for RT-qPCR ( Table 1 ). The forward and reverse primers of house-keeping genes (glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ] and 18S ribosomal RNA [ 18S ]); genes related to immunity including interleukin ( IL ) -2 , IL-6 , IL-10 , interferon gamma ( IFN-γ ), myeloid differentiation factor 88 ( MyD88 ), transforming growth factor beta 4 ( TGF-β4 ), Toll-like receptor ( TLR )-4, and TLR-15 ; and genes linked to vitamin D activity including 1α-hydroxylase, 24-hydroxylase, 25-hydroxylase, and VDR were designed according to the procedure described by Fatemi [ 31 ] and Poudel et al [ 32 ]. NormFinder software version 20 was used to normalize the target gene data [ 33 ], and the normalization suitability of the expression of GAPDH and 18S genes were tested.…”

Section: Methodsmentioning

confidence: 99%

Improvement in the Immunity- and Vitamin D3-Activity-Related Gene Expression of Coccidiosis-Challenged Ross 708 Broilers in Response to the In Ovo Injection of 25-Hydroxyvitamin D3

Fatemi

Macklin

Zhang

et al. 2022

Animals

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Effects of the in ovo administration of two vitamin D3 sources (vitamin D3 (D3) and 25-hydroxyvitamin D3 (25OHD3)) on the expression of D3 activity- and immunity-related genes in broilers subjected to a coccidiosis infection were investigated. At 18 d of incubation (doi), five in ovo injection treatments were administrated to live embryonated Ross 708 broiler hatching eggs: non-injected (1) and diluent-injected (2) controls, or diluent injection containing 2.4 μg of D3 (3) or 2.4 μg of 25OHD3 (4), or their combination (5). Birds in the in ovo-injected treatments were challenged at 14 d of age (doa) with a 20× dosage of a live coccidial vaccine. At 14 and 28 doa, the expression of eight immunity-related genes (IL-2, IL-6, IL-10, TLR-4, TLR-15, MyD88, TGF-β4, and IFN-γ) and four D3 activity-related genes (1α-hydroxylase, 25-hydroxylase, 24-hydroxylase, and VDR) in the jejunum of one bird in each treatment–replicate group were evaluated. No significant treatment effects were observed for any of the genes before challenge. However, at 2 weeks post-challenge, the expression of 1α-hydroxylase, TGF-β4, and IL-10 increased in birds that received 25OHD3 alone in comparison to all the other in ovo-injected treatment groups. Additionally, the expression of 24-hydroxylase and IL-6 decreased in birds that received 25OHD3 in comparison to those injected with diluent or D3 alone. It was concluded that the in ovo injection of 2.4 μg of 25OHD3 may improve the intestinal immunity as well as the activity of D3 in Ross 708 broilers subjected to a coccidiosis challenge.

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Prevalence, Antimicrobial Resistance, and Molecular Characterization of Campylobacter Isolated from Broilers and Broiler Meat Raised without Antibiotics

Abstract: Campylobacter is one of the main bacterial pathogens that cause campylobacteriosis in the United States. Poultry is considered a major reservoir for the transmission of Campylobacter to humans.

Cited by 27 publications

References 49 publications

Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

Antimicrobial Resistance Challenged with Platinum(II) and Palladium(II) Complexes Containing 1,10-Phenanthroline and 5-Amino-1,3,4-Thiadiazole-2(3H)-Thione in Campylobacter jejuni

Improvement in the Immunity- and Vitamin D3-Activity-Related Gene Expression of Coccidiosis-Challenged Ross 708 Broilers in Response to the In Ovo Injection of 25-Hydroxyvitamin D3

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