Prevalence, Antimicrobial Resistance and Substantial Virulence-Associated Genes of Escherichia Coli Isolated From Colibacillosis in Neonatal Calves in Egypt

Mousa, Walid S.

doi:10.15414/jmbfs.2020.9.6.1145-1150

“…In a previous study from Egypt, El-Seedy et al [ 26 ] reported a frequency rate of 18.1% and 75.6% for Salmonella serovars and E. coli in diarrheic calves, respectively. Also, in line with the current findings, Mousa et al [ 27 ] and Shekhar et al [ 28 ] reported a frequency rate of 40.0% and 41.6% for E. coli isolates in samples from calves with diarrheal disease, respectively. On contrary, the current result was lower than previous studies by Tadesse et al [ 29 ] and Mohammed et al [ 30 ], who reported prevalence rates of 49%, 85%, and 46.4% for E. coli , respectively.…”

Section: Discussionsupporting

confidence: 91%

Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

Hasson¹,

Judi

²

,

Salih

³

et al. 2022

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Objectives This study aimed to evaluate the association of the intimin (eae) and pagC genes with biofilm formation and multidrug resistance (MDR) phenotype in Escherichia coli and Salmonella enterica collected from calves with diarrhea. Results Fecal samples (n: 150) were collected from calves with diarrhea. Of 150 fecal samples, 122 (81.3%) were culture positive and 115/122 (94.2%) were Gram-negative bacteria. Among them, E. coli (n = 64/115, 55.6%) was the most common isolate followed by S. enterica (n = 41/115, 35.6%). Also, 10 (8.6%) isolates were other Enterobacteriaceae bacteria including Klebsiella and Proteus species. Eighty-nine isolates (77.4%) from calf diarrhea, including 52 (81.3%) E. coli and 37 (90.2%) S. enterica were MDR. The eae and pagC genes were detected in 33 (51.5%) E. coli and 28 (68.3%) S. enterica isolates, respectively. There was a strong association between these genes and biofilm formation and MDR phenotype (P-value = 0.000). All E. coli isolates carrying the eae gene were biofilm producers and MDR. Also, all pagC-positive S. enterica isolates were MDR and 25 (89.3%) isolates of them produced biofilm.

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“…The pathogenicity of E. coli depends mainly on the presence of different virulence genes and the existence of several toxins (Mousa et al, 2020). In the present study, out of 12 E. coli strains, only 8% from fuchka contained the virulence gene hlyA characteristic for EHEC and belonged to virulent phylogroup D (Figure 5).…”

Section: Incidence Of Virulence Genes In E Colimentioning

confidence: 53%

PHYLOTYPING, PATHOTYPING and PHENOTYPIC CHARACTERISTICS of Escherichia coli ISOLATED from VARIOUS STREET FOODS in BANGLADESH

Bhowmik

¹

,

Goswami

²

,

Sirajee

³

et al. 2022

J microb biotech food sci

1

0

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The burden of street food contamination remains significantly higher among developing countries, making its consumers' health compromised. This study aimed at identifying Escherichia coli in local street foods, track their sources and characterize their virulence properties, antimicrobial susceptibility patterns, and incidence of recent fecal contamination. Twelve E. coli isolates isolated from 28 types of street foods sold at different locations of Dhaka metropolitan city were confirmed by both culture-based and molecular methods. Phylogroup B1 of environment origin was the most predominant (58%, n=12) among the isolates, followed by commensal phylogroup A (16%), phylogroup C (8%), D (8%), and E (8%). Alarmingly, 8% of the isolates were confirmed to be Enteropathogenic E. coli (EPEC), harboring hlyA gene, and 41% were due to recent fecal contamination cases confirmed by the presence of the eae gene. Isolates showed the highest resistance to ampicillin, followed by chloramphenicol. However, resistance could not be correlated to the presence of class 1 integron as isolates sensitive to these antibiotics also harbored this mobile genetic element. The findings of this study demonstrated the presence of antibiotic-resistant, potentially pathogenic E. coli in street food which emphasizes the health hazard associated with consumption of such foods.

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“…E. coli was found in velpuri (35%, n=20), masalapuri (30%, n=10), panipuri (30%, n=10), sevpuri (20%, n=5), and lime rice (20 percent, n=5), according to Bhaskar et al (2004). Sample varieties, geographical locations, stress factors, sanitary management systems, and other factors all impacted the study's outcomes (Mousa et al, 2020). In the present study, since street foods are mostly human handled in Bangladesh and vendors do not have enough concern about maintaining proper hygienic practices, we hypothesize that unclean hands' improper handling led to E. coli in the samples studied.…”

Section: Prevalence Of E Coli In Food Samplesmentioning

confidence: 99%

“…Therefore multidrug-resistant E. coli has become a public health concern nowadays as this microbe can become an opportunistic pathogen. Its pathogenic mechanism includes adhesion and binding to the small intestine’s mucosal receptor (Mousa and Shama, 2020 ) . One of the virulence genes of E. coli, hly A, which encodes Hemolysin A can create pores in the cell membranes of host cells and result in cell lysis (Sarowska et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phylotyping, Pathotyping and Phenotypic Characteristics of Escherichia coli Isolated from Various Street Foods in Bangladesh

Bhowmik

¹

,

Goswami

²

,

Sirajee

³

et al. 2022

Preprint

0

View full text Add to dashboard Cite

The burden of street food contamination remains significantly higher among developing countries, making its consumers' health compromised. This study aimed at identifying Escherichia coli in local street foods, track their sources and characterize their virulence properties, antimicrobial susceptibility patterns, and incidence of recent fecal contamination. Twelve E. coli isolates isolated from 28 types of street foods sold at different locations of Dhaka metropolitan city were confirmed by both culture-based and molecular methods. Phylogroup B1 of environment origin was the most predominant (58%, n=12) among the isolates, followed by commensal phylogroup A (16%), phylogroup C (8%), D (8%), and E (8%). Alarmingly, 8% of the isolates were confirmed to be Enteropathogenic E. coli (EPEC), harboring hlyA gene, and 41% were due to recent fecal contamination cases confirmed by the presence of the eae gene. Isolates showed the highest resistance to ampicillin, followed by chloramphenicol. However, resistance could not be correlated to the presence of class 1 integron as isolates sensitive to these antibiotics also harbored this mobile genetic element. The findings of this study demonstrated the presence of antibiotic-resistant, potentially pathogenic E. coli in street food which emphasizes the health hazard associated with consumption of such foods.

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Prevalence, Antimicrobial Resistance and Substantial Virulence-Associated Genes of Escherichia Coli Isolated From Colibacillosis in Neonatal Calves in Egypt

Cited by 8 publications

References 47 publications

Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

PHYLOTYPING, PATHOTYPING and PHENOTYPIC CHARACTERISTICS of Escherichia coli ISOLATED from VARIOUS STREET FOODS in BANGLADESH

Phylotyping, Pathotyping and Phenotypic Characteristics of Escherichia coli Isolated from Various Street Foods in Bangladesh

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