Prevalence, Genotypic Characteristics and Antibiotic Resistance of Listeria monocytogenes From Retail Foods in Bulk in Zhejiang Province, China

Zhang, Yunyi; Dong, Shengyi; Chen, Honghu; Chen, Jiancai; Zhang, Junyan; Zhang, Zhen; Yang, Yong; Zhang, Xu; Li, Zhan; Mei, Lingling

doi:10.3389/fmicb.2019.01710

Cited by 42 publications

(26 citation statements)

References 82 publications

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“…13,[21][22][23][24][25][26] An investigation on the prevalence of L. monocytogenes in retail foods showed that ST9 (23.3%), ST155 (16.4%), and ST8 (12.3%) were the main dominant types in Zhejiang province. 27 Their observations combined the results from this work imply the epidemiological difference between clinical and food isolates from Zhejiang province, although prevalent serotypes 1/2a, 1/2b, and 4b were also identified in that study. Of note, ST87 clone was the predominant ST in clinical L. monocytogenes isolates and closely related to pregnancy-associated infections in China.…”

Section: Discussionsupporting

confidence: 68%

Genomic and Phenotypic Diversity of Listeria monocytogenes Causing Pregnancy-Associated Listeriosis from Zhejiang Province, China, 2016–2018

Li¹,

Xu²,

Li³

et al. 2020

IDR

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Introduction: There are few investigations describing the pregnancy-associated listeriosis in China, and the molecular characteristics of Listeria monocytogenes causing such infections remain largely unknown. We aim to investigate the phenotypic and genomic profiles of pregnancy-associated L. monocytogenes isolates and their association with isolates recovered from human and non-human in China. Materials and Methods: In this study, we conducted a 3-year surveillance of listeriosis in a women's hospital in Zhejiang province, using whole genome sequencing and bioinformatics tools. Results: From 2016 to 2018, we identified 13 clinical L. monocytogenes isolates. Among these pregnancy-associated isolates, we found seven sequence types (STs), with the prevalent STs of ST87 and ST7. Serotyping divided the strains into four serotypes, including serotype 1/2a, 1/2b, 3a, and 4b. Antimicrobial resistance testing showed that all the isolates were susceptible to 10 antibiotics. Comparative genomics analysis clearly classified our genome collection into four distinct evolutionary lineages with most isolates grouping into lineages I and II. Interestingly, we found three pairs of isolates with high identity, although no evident epidemiological association was observed. Conclusion: This study reports for the first time the surveillance of pregnancy-associated listeriosis in Zhejiang province, China, which indicates that the infection rate is low in this region. Our findings provide insight into the evolution and genetic diversity of pregnancyassociated L. monocytogenes from Zhejiang province. Additional investigations involving more human and non-human isolates with a "one health" strategy are needed for prediction of the listeriosis risk associated with a typical prevalent clone in Zhejiang province, such as ST87.

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Section: Discussionsupporting

confidence: 68%

Genomic and Phenotypic Diversity of Listeria monocytogenes Causing Pregnancy-Associated Listeriosis from Zhejiang Province, China, 2016–2018

Li¹,

Xu²,

Li³

et al. 2020

IDR

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“…In our research, a small number of isolates belonged to ST9/CC9, ST91/CC14, and ST141/CC155 were newly found to harboured llsX and/or ptsA, suggesting that they may pose a hyper-pathogenic risk to public health. Those LIPI-3 and/or LIPI-4-positive isolates belonged to lineage II, which was inconsistent with the finding that LIPI-3 was identified exclusively in a subset of lineage I [44]. It is probably because strains in farm or slaughtering environment have evolved these two genes through gene transfer under natural environmental pressures.…”

Section: Discussionmentioning

confidence: 66%

Prevalence and transmission characteristics of Listeria species from ruminants in farm and slaughtering environments in China

Zhao

et al. 2021

Emerging Microbes & Infections

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Listeria monocytogenes is an important foodborne pathogen, and is ubiquitously distributed in the natural environment. Cattle and sheep, as natural hosts, can transmit L. monocytogenes to related meat and dairy products. In this study, the prevalence, distribution, and transmission characteristics of Listeria were analysed by investigating 5214 samples of cattle and sheep in farm and slaughtering environments in China. A low contamination incidence of L. monocytogenes (0.5%, 20/4430) was observed in farm environment, but there was a high contamination incidence in slaughtering environment (9.4%, 74/784). The incidence of L. innocua in cattle and sheep farm and slaughtering environments is more common and significantly higher (9.7%, 508/5214) than that of L. monocytogenes (1.8%, 94/5214). The distinct molecular and genetic characteristics of Listeria by PFGE and MLST indicated that L. monocytogenes and L. innocua were gradually transmitted from the farm and slaughtering environments to end products, such as beef and mutton along the slaughtering chain. The ST7, ST9, ST91, and ST155 found in our study were associated with the human listeriosis cases in China. In addition, the findings of virulence markers (inlC, inlJ, LIPI-3, LIPI-4, and ECIII) concerned with the pathogenesis of human listeriosis and antibiotics resistance of L. monocytogenes in this study implies a potential public health risk. This study fills the gap in the epidemiology of beef cattle and sheep that carry Listeria in farm and slaughtering environments in major cattle and sheep producing areas in China.

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“…In Mexico, L. monocytogenes was present in 16/79 (20.3%) pork samples collected from two slaughterhouses from 2014 to 2015 in southern Sonora ( Figueroa-López et al, 2019 ). In China, L. monocytogenes contaminations in retail raw pork (11.68–30%), chilled pork (11–55%), and RTE pork (4.61–13.5%) have been reported in recent years ( Yin et al, 2015 ; Li et al, 2018 ; Chen et al, 2019 ; Zhang et al, 2019b ). Pork is the most frequently consumed meat in China, the main consumption habit is to take cooked pork.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Diversity of Listeria monocytogenes Isolated From Retail Pork in Wuhan, China

Yiqian

et al. 2021

Front. Microbiol.

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Listeria monocytogenes is a ubiquitous bacteria and causative agent of zoonotic listeriosis with high mortality. The consumption of contaminated animal-derived foods has been linked with both epidemic and sporadic listeriosis. In this work, a total of 64 L. monocytogenes isolates from 259 pork samples sold in 11 supermarket chains were identified and characterized by comparative whole-genome analysis. All isolates were delineated into eight clonal complexes (CCs), namely CC2, CC8, CC9, CC11, CC155, CC121, CC204, and CC619, spanning two lineages (I and II) and carrying 3–5 antibiotic-resistant genes (fosX, lnu, mprF, tetM, and dhfR). It is noted that Listeria pathogenicity island (LIPI)-1, LIPI-3, and LIPI-4 were distributed in all ST619 isolates from two supermarket chains that were closely related with clinical isolates (<40 SNP). Some of the isolates from different supermarket chains with 0 SNP difference indicated a common pork supply source. Notably, 57.81% of the strains carried types IB, IIA, or IIIB CRISPR-Cas system, CC121 isolates carried both types IB and IIA CRISPR-Cas systems, Cas proteins of CC155 isolates located between two CRISPR loci, each CC has unique organization of Cas proteins as well as CRISPR loci. CRISPR-Cas system-based subtyping improved discrimination of pork-derived L. monocytogenes isolates. Comparisons at the genome level contributed to understand the genetic diversities and variations among the isolates and provided insights into the genetic makeup and relatedness of these pathogens.

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Prevalence, Genotypic Characteristics and Antibiotic Resistance of Listeria monocytogenes From Retail Foods in Bulk in Zhejiang Province, China

Cited by 42 publications

References 82 publications

<p>Genomic and Phenotypic Diversity of <em>Listeria monocytogenes</em> Causing Pregnancy-Associated Listeriosis from Zhejiang Province, China, 2016–2018</p>

<p>Genomic and Phenotypic Diversity of <em>Listeria monocytogenes</em> Causing Pregnancy-Associated Listeriosis from Zhejiang Province, China, 2016–2018</p>

Prevalence and transmission characteristics of Listeria species from ruminants in farm and slaughtering environments in China

Prevalence and Genetic Diversity of Listeria monocytogenes Isolated From Retail Pork in Wuhan, China

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