Prevalence, identification of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxin producing Escherichia coli strains isolated from raw milk and traditional dairy products

Abstract

BackgroundShiga-toxigenic Escherichia coli strains are one of the most important foodborne bacteria with an emergence of antibiotic resistance. Foodborne STEC strains are mainly associated with presence of certain virulence factors and O-seogroups. The present investigation was done to study the distribution of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxigenic Escherichia coli isolated from milk and dairy products.MethodsSix-hundred samples were randomly collected and immediately transferred to laboratory. All samples were cultured and E. coli strains were isolated. STEC strains were identified based on the presence of putative virulence factors and subtypes. STEC isolates were subjected to multiplex PCR and disk diffusion methods.ResultsOne-hundred and eighty-one out of 600 samples (30.16%) harbored E. coli. Prevalence of STEC strains was 10.66%. O157 (43.75%) and O26 (37.50%) were the most frequently identified serogroups. Aac(3)-IV (100%), CITM (96.87%) and tetA (76.56%) were the most commonly detected antibiotic resistance genes. STEC strains had the highest prevalence of resistance against ampicillin (100%), gentamicin (100%) and tetracycline (96.87%).ConclusionsKashk and dough were negative for presence of E. coli strains. High prevalence of resistant-O157 strains and simultaneous presence of multiple virulence factors pose an important public health problem regarding the consumption of raw milk and dairy products.

Retracted: Vacuolating cytotoxin A (vacA) and cytotoxin‐associated gene A (cagA) genotypes of resistant Helicobacter pylori strains isolated from raw and pasteurized milk

“…In despite of the high importance of H. pylori , occurrence and antibiotic resistance of other foodborne pathogens have been reported previously from Iran (Madahi, Rostami, Rahimi, & Dehkordi, ; Momtaz, Davood Rahimian & Safarpoor Dehkordi, 2013; Momtaz, Dehkordi, Rahimi, Asgarifar, & Momeni, ; Momtaz, Safarpoor Dehkordi, Taktaz, Rezvani, & Yarali, ; Rahimi, Yazdanpour, & Dehkordi, ; Ranjbar, Masoudimanesh, Dehkordi, Jonaidi‐Jafari, & Rahimi, ; Ranjbar, Safarpoor Dehkordi, Sakhaei Shahreza, & Rahimi, , Ranjbar, Yadollahi Farsani, et al, ; Safarpoor Dehkordi, Barati, et al, ; Safarpoor Dehkordi, Gandomi, Akhondzadeh Basti, Misaghi, & Rahimi, ; Safarpoor Dehkordi, Haghighi, Momtaz, Rafsanjani, & Momeni, ; Safarpoor Dehkordi, Khamesipour, & Momeni, ; Safarpoor Dehkordi et al, ; Safarpoor Dehkordi, Valizadeh, Birgani, & Dehkordi, ).…”

Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

“…Enterohemorrhagic E. coli (EHEC) bacteria are a dangerous phenomenon originated from Shiga toxin-producing E. coli (STEC) [3][4][5]. They are accountable for hemolytic uremic syndrome (HUS), thrombotic thrombocytopenic purpura (TTP), hemorrhagic colitis (HC) and diarrhea [6,7]. O157 is a chief serogroup of the above mentioned phenomena with boost clinical standing [3][4][5][6][7].…”

“…They are accountable for hemolytic uremic syndrome (HUS), thrombotic thrombocytopenic purpura (TTP), hemorrhagic colitis (HC) and diarrhea [6,7]. O157 is a chief serogroup of the above mentioned phenomena with boost clinical standing [3][4][5][6][7]. Poultry is one of the most significant sources of human infection [4].…”

“…coli O157 bacteria harbored the boost incidence of intimin (eaeA), Shiga toxins (stx1 and stx2) and hemolysin (hlyA). They act as adhesive and invasive factors and are chiefly accountable for occurrence of attaching-effacing (A/E) lesions which mainly caused by the eaeAgene [3][4][5][6][7]. Egypt These markers are chiefly accompanying with occurrence of clinical syndromes and are attended with bacterial adhesion and invasion to host cells.…”

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

“…In keeping with the high nutritional importance of dairy products (Thorning et al, 2016), dairy products are not essentially safe, as demonstrated by higher rates of foodborne illnesses associated with their consumption (Momtaz, Safarpoor Dehkordi, Taktaz, Rezvani, & Yarali, 2012;Rahimi, Sepehri, Dehkordi, Shaygan, & Momtaz, 2014;Ranjbar, Dehkordi, Shahreza, & Rahimi, 2018;Safarpoor Dehkordi, Barati, Momtaz, Hosseini Ahari, & Nejat Dehkordi, 2013;Safarpoor Dehkordi, Valizadeh, Birgani, & Dehkordi, 2014). As well, there were so many investigations about the considerable incidence of some specific foodborne pathogens in dairies (Momtaz et al, 2012;Ranjbar, Yadollahi Farsani, & Safarpoor Dehkordi, 2018;Safarpoor Dehkordi et al, 2013;Safarpoor Dehkordi et al, 2014).…”