Prevalence, intensity and histopathology of <i>Zeylanicobdella arugamensis</i> infestation on groupers reared on different aquaculture systems

Mahasri, Gunanti; Hafidloh, Umi; Pratama, Fungky Putra; Rahmawan, Dicky; Subekti, Sri; Sari, Putri Desi Wulan; Amin, Muhamad

doi:10.1111/jfd.13219

Cited by 6 publications

(6 citation statements)

References 38 publications

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“…This species has also been reported to use cultured groupers as its hosts [ 12 , 13 , 14 , 15 , 16 ], which provides further support to the identification. The observed morphological characteristics have also been described by Cruz-Lacierda et al [ 12 ], Murwantoko et al [ 15 ], Nagasawa and Uyeno [ 23 ], Chandra [ 39 ], Sawyer [ 40 ], Burreson [ 17 ], Rahayu et al [ 41 ] and Mahasri et al [ 42 ].…”

Section: Resultssupporting

confidence: 75%

Population Genetic Structure of Marine Leech, Pterobdella arugamensis in Indo-West Pacific Region

Azmey

Taha

Mahasri

et al. 2022

Genes

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Grouper aquaculture is rapidly expanding in both tropical and subtropical regions. The presence of marine leeches (Pterobdella arugamensis; previously named Zeylanicobdella arugamensis) infesting cultured groupers, however, can have a fatal effect on grouper aquaculture production and cause significant economic loss. Understanding the marine leech’s population structure is therefore important to determine its possible distributional origin and distributional mechanisms, which will help monitor and mitigate the infestation. In this study, a total of 84 marine leeches collected from cultured hybrid groupers Epinephelus spp. in Brunei Darussalam, Malaysia and Indonesia were identified as P. arugamensis, based on morphological and mitochondrial cytochrome c oxidase subunit I gene sequence analyses. These leech samples, together with additional sequences from the GenBank database, were grouped into four genetically distinct haplogroups: (1) Asia Pacific, (2) Borneo, (3) Surabaya and (4) Iran. The four populations were found to be highly diverged from each other. The results also suggested that the samples from the Asia Pacific population could be dispersed and transported from Indonesia.

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Section: Resultssupporting

confidence: 75%

Population Genetic Structure of Marine Leech, Pterobdella arugamensis in Indo-West Pacific Region

Azmey

Taha

Mahasri

et al. 2022

Genes

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“…The cocoon development phase is in accordance with the cocoon development phase. The morula to gastrula phase occurs for 3-5 days followed by the embryo phase until hatching on days 10-12 at a rearing temperature of 28-31°C and salinity of 34-35 ppt, but cannot hatch at 40°C [13,17,19,25,30]. Cocoons in citric acid immersion at concentrations of 250 ppm and 400 ppm had no nucleus and did not develop until the end of the study.…”

Section: Effectiveness Of Citric Acid Soaking On Cocoons Of Z Arugame...mentioning

confidence: 93%

“…Infestation of ectoparasitic worms in the gills results in hyperplasia and fusion of the gill lamellae. Affected host fish will be thickened/swollen and pale red in color [15,[19][20][21]. Z. arugamensis in citric acid immersion at a concentration of 300 ppm attached to the body of the grouper appears to be released to the bottom of the water in a shrunken and dead condition.…”

Section: Safety Of Citric Acid Immersion For Hybrid Grouper Infected ...mentioning

confidence: 99%

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The Effectiveness Of Citric Acid As An Anti-Ectoparasite Of Marine Leech (Zeylanicobdella arugamensis) Through Soaking

Nike Mustika Sari,

Gede Iwan Setiabudi,

Indah Mastuti

et al. 2023

ATBES

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Zeylanicobdella arugamensis infection causes health problems for cultured grouper. This study aims to determine the effectiveness of citric acid soaking as an anti Z. arugamensis ectoparasite. A total of ±120 Z. arugamensis were isolated from sick hybrid grouper (Epinephelus fuscoguttatus x E. lanceolatus) placed in Petri dishes (6 Petri dishes). Citric acid solution with 0, 50, 100, 150, 250, and 400 ppm was added to the petri plates. Immersion of Z. arugamensis was carried out for 30, 60, 90 and 120 minutes. Each 30 Z. arugamensis/treatment/30 minutes was taken and placed in 3 petri dishes (10 individuals/petri dish) filled with seawater. Observations were made on the response of Z. arugamensis. Comments were made on cocoon development into larvae. The citric acid safety test was conducted on cantang hybrid grouper infected with Z. arugamensis. A total of 3 test fish were soaked with citric acid solution in seawater at concentrations of 0, 150, 250, and 300 ppm. Observations of fish condition were made after 30 and 60 minutes of immersion. The results showed that citric acid concentrations of 250 and 400 ppm effectively killed Z. arugamensis (76.67±15.28-100%), and their cocoon development reached (0%). A citric acid concentration of 300 ppm is still safe to use for immersion of cantang hybrid grouper infected with Z. arugamensis for 60 minutes. Citric acid concentrations of 250-300 ppm can also release Z. arugamensis from the fish body. Citric acid has antiparasitic properties and lowers the pH of seawater.

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“…Sequencing tests of Benedenia specimens were carried out at 1st Based Singapore through the Professor Nidom Foundation Laboratory Surabaya, Indonesia. Prevalence and intensity of Benedenia were evaluated as a previous study procedure (Mahasri et al 2020). Prevalence is the percentage of infested fish compared to the total sample of fish examined.…”

Section: Collection Site For Samples and Datamentioning

confidence: 99%

First microphological and molecular parasitological survey of Benedenia in humpback grouper (Cromileptes altivelis) of Lampung and Situbondo, Indonesia

AMIIN,

SUBEKTI,

MASITHAH

et al. 2024

Biodiversitas

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Abstract. Amiin MK, Subekti S, Masithah ED, Nirmala D, Yunus M, Santanumurti MB, Rivaie AR. 2023. First microphological and molecular parasitological survey of Benedenia in humpback grouper (Cromileptes altivelis) of Lampung and Situbondo, Indonesia. Biodiversitas 24: 6858-6867. Capsalid monogenean infections, such as Benedenia, affect grouper aquaculture production. Benedenia will cause lesions and skin damage which will cause fish prices to drop. Secondary infection from bacteria, viruses, and fungi by Benedenia triggers a slow growth rate and even mortality. Benedenia infection on grouper has been conducted in Indonesia, specifically in this study in Lampung and Situbondo. However, molecular identification of this parasite is still lacking. This study aims to determine the microscopic and molecular characteristics of Benedenia from the Hurun Bay waters of Lampung and the North Coast of the Java Sea, Situbondo. The main parameters observed in this study were DNA base sequence, morphology, and phylogenetic tree. Benedenia from Lampung (637 bp) and Situbondo (741 bp) in this study was confirmed by the morphological and molecular close as Benedenia sargocentron with nucleotides. Interestingly, the similarity was less than 98% with B. sargocentron with accession ID JN797597.1 (similarity of 97.15% from Lampung and 86.33% from Situbondo). It could be indicated as a new species of Benedenia. The prevalence of Benedenia in Lampung was 90%, while Situbondo showed 80% with 3 parasite/fish intensity for each location. The outcome of this study is the characteristics of Benedenia from Lampung and Situbondo were similar to B. sargocentron according to morphological and molecular identification. This is the first report of B. sargocentron from Lampung and Situbondo since no molecular identification has been done in those places to confirm their species. Further research by another molecular target apart from 28S rRNA should be needed to identify this parasite more accurately.

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Prevalence, intensity and histopathology of Zeylanicobdella arugamensis infestation on groupers reared on different aquaculture systems

Cited by 6 publications

References 38 publications

Population Genetic Structure of Marine Leech, Pterobdella arugamensis in Indo-West Pacific Region

Population Genetic Structure of Marine Leech, Pterobdella arugamensis in Indo-West Pacific Region

The Effectiveness Of Citric Acid As An Anti-Ectoparasite Of Marine Leech (Zeylanicobdella arugamensis) Through Soaking

First microphological and molecular parasitological survey of Benedenia in humpback grouper (Cromileptes altivelis) of Lampung and Situbondo, Indonesia

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