Prevalence, molecular detection, and virulence gene profiles of Campylobacter species in humans and foods of animal origin

Barakat, Ashraf M.; El-Razik, Khaled A. Abd; Elfadaly, Hassan A.; Rabie, Nagwa S.; Sadek, Sabry A. S.; Almuzaini, Abdulaziz M.

doi:10.14202/vetworld.2020.1430-1438

Cited by 16 publications

(9 citation statements)

References 47 publications

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“…A study by Zeinhom et al in 2019 reported a C. jejuni isolation rate of 48% (12/25) from individuals with diarrhea across Beni-Suef Governorate; however, the detection rate was only slightly higher than that from non-diarrheic individuals (32.1%, 9/28) (Zeinhom et al, 2021). Barakat et al reported that 38.1% (40/105) of diarrheic human stools collected in 2018 from five different governorates were PCR positive for Campylobacter, among which a majority of them were C. jejuni(92.5%, 37/40) (Barakat et al, 2020). A similar detection rate of C. jejuni was reported in Sharkia (30%, 30/100) and Ismailia (31.2%, 25/80) Governorates between (Ammar et al, 2020Abdelmageed et al, 2021).…”

Section: Egyptmentioning

confidence: 99%

Global epidemiology of campylobacteriosis and the impact of COVID-19

Liu

Lee

Xue

et al. 2022

Front. Cell. Infect. Microbiol.

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Campylobacteriosis is a gastroenteritis caused by pathogenic Campylobacter species and an important topic in public health. Here we review the global epidemiology of campylobacteriosis in the last eight years between 2014-2021, providing comprehensive and updated information on the reported incidence and outbreaks of Campylobacter infections. The government public health website of each of the 195 countries and publications from 2014 to September 2022 in public databases were searched. The reported incidence of campylobacteriosis in pre-COVID-19 years was compared to that during the COVID-19 pandemic in countries where data were available. Czech Republic had the highest reported incidence of campylobacteriosis worldwide (215 per 100,000 in 2019), followed by Australia (146.8 per 100,000 in 2016) and New Zealand (126.1 per 100,000 in 2019). Campylobacter was one of the most common human enteric pathogens in both developed and developing countries. About 90% of cases of campylobacteriosis were caused by Campylobacter jejuni, whereas less than 10% of cases were caused by Campylobacter coli. Other Campylobacter species were also isolated. The reported incidence and case numbers of campylobacteriosis in developed nations have remained steadily high prior to the COVID-19 pandemic, whilst some countries reported an increasing trend such as France and Japan. While outbreaks were more frequently reported in some countries, Campylobacter infections were mainly sporadic cases in most of the developed countries. Campylobacter infection was more common in summer in some but not all countries. Campylobacter infection was more common in males than females. The COVID-19 pandemic has reduced the reported incidence of campylobacteriosis in most countries where 2020 epidemiology data were available. In conclusion, Campylobacter infection remains a global health concern. Increased research and improved strategies are needed for prevention and reduction of Campylobacter infection.

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Section: Egyptmentioning

confidence: 99%

Global epidemiology of campylobacteriosis and the impact of COVID-19

Liu

Lee

Xue

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Also, Barakat et al, [ 40 ] reported that multiplex PCR is a valuable method for detection of virulent and resistant genes of Campylobacter bacteria.…”

Section: Discussionmentioning

confidence: 99%

Multiplex PCR: Aid to more-timely and directed therapeutic intervention for patients with infectious gastroenteritis

et al. 2022

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Background: Multiplex PCR is a sensitive and rapid method compared with conventional methods. Therefore, we use multiplex PCR for the rapid detection of the four major intestinal pathogens causing gastroenteritis ( Shigella spp., Campylobacter spp., Aeromonas spp. and Enterohemorrhagic Escherichia coli [EHEC]) in stool specimens. Materials and methods: A prospective randomized study using 200 stool samples obtained from patients presented with acute gastroenteritis during the study period (between February 2019 and December 2021). Bacteria in stool samples were identified using conventional culture methods and multiplex PCR for stool samples Results: The identified organisms using conventional cultures; were Shigella (27%), Aeromonas species (10%) and EHEC (O157 ) (8%). Using multiplex PCR. Shigella spp. was the most commonly identified pathogen (detected in 40.5% of positive samples), followed by Aeromonas spp. (30%), EHEC (20%) and Campylobacter species was only detected in (1%) of positive samples. The diagnostic evaluation of multiplex PCR in relation to conventional method in diagnosis of Shigella, EHEC and Aeromonas showed, sensitivity of 100% (for each), specificity of 88.5%, 92.4%, 77.8% respectively. However, the diagnostic evaluation of multiplex PCR in relation to conventional method in diagnosis of Campylobacter showed specificity of 99% and NPV of 100%. Conclusions: Multiplex PCR is an accurate and rapid method for detection of common intestinal pathogens causing severe gastroenteritis. a rapid method that could be used in outbreaks for diagnosis of the common enteric pathogens causing fatal gastroenteritis.

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“…W hile in the present study 12 (63.15%) chicken meat samples were positive on the basis of biochemical testes and 10 (83.33%) by PCR targeting map A gene and hip O genes. Chicken samples were bacteriologically positive for Campylobacter isolates and 285 isolates (94.37%) were identified as C. jejuni by polymerase chain reaction targeting the hip O gene specific for C. jejuni (Barakat et al, 2020).…”

Section: Genotypic Characterizationmentioning

confidence: 99%

Morphological, Biochemical and Genotypic Analysis of Zoonotic Campylobacter jejuni Isolated from Chicken Meat Samples

Bobade

Vijayarani

Tirumurugaan

et al. 2022

IJAR

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Background: Campylobacter species are a leading cause of most important food-borne diarrhoeal illness worldwide while, poultry has been identified as a significant cause of Campylobacter infection in humans. C. jejuni is highly effective in colonizing chicken intestinal mucosa without causing any clinical manifestations and the consumption of poultry meat is the major source of transmission of bacteria to humans. Methods: The total of 19 chicken meat samples collected from retail markets in Chennai were screened by cultural examination, further subjected to phenotypic characterization using biochemical test and genotypic characterization using polymerase chain reaction assay targeting hip O and map A genes. Result: All the isolates showed growth on modified blood free charcoal cefoperazone deoxycholate agar media (mCCDA) and 18 (94.73%) samples showed typical morphological characteristics. The 12 (63.15%) isolates showed biochemical reactions positive. The results from polymerase chain reaction showed that 10 (83.33%) isolates were positive for C. jejuni. This study suggested that, it is essential to investigate the incidence of Campylobacter jejuni infection in poultry and the risk factors at all production stages of meat production to help reducing the disease in humans in terms of food safety.

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Prevalence, molecular detection, and virulence gene profiles of Campylobacter species in humans and foods of animal origin

Cited by 16 publications

References 47 publications

Global epidemiology of campylobacteriosis and the impact of COVID-19

Global epidemiology of campylobacteriosis and the impact of COVID-19

Multiplex PCR: Aid to more-timely and directed therapeutic intervention for patients with infectious gastroenteritis

Morphological, Biochemical and Genotypic Analysis of Zoonotic Campylobacter jejuni Isolated from Chicken Meat Samples

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