Prevalence, Numbers and Antimicrobial Susceptibilities of Salmonella Serovars and Campylobacter spp. in Retail Poultry in Phnom Penh, Cambodia

Lay, Kruy Sun; Vuthy, Yith; Song, Ping; Phol, Khem; Sarthou, Jean Louis

doi:10.1292/jvms.10-0373

Cited by 55 publications

(41 citation statements)

References 17 publications

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“…In our study, only 4% of 779 Salmonella isolates were susceptible to the antimicrobial agents tested; 96% of the isolates were resistant to at least 1 antimicrobial agent, 57% were resistant to at least 5 agents, and 28% were resistant to at least 10 agents. High percentages of antimicrobial-resistant Salmonella isolates from poultry and poultry products also have been reported by other investigators (5,8,19,20,27,34). Of the five most common serotypes identified in our study, the highest percentage of Salmonella Indiana isolates were multidrug resistant, whereas Salmonella Enteritidis was less resistant to antimicrobial agents, in agreement with previous investigations of Salmonella isolates recovered from retail meats and food animals (27,37).…”

Section: Discussionsupporting

confidence: 93%

Counts, Serotypes, and Antimicrobial Resistance of Salmonella Isolates on Retail Raw Poultry in the People’s Republic of China

Yang

Cui

Shi

et al. 2014

Journal of Food Protection

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The objective of this study was to determine Salmonella counts, serotypes, and antimicrobial resistance profiles in retail raw chicken meat in the People's Republic of China. Salmonella counts were determined according to the most-probable-number (MPN) method for 300 whole chicken carcasses. These samples were collected from large, small, and wet (open) markets in Guangdong, Shaanxi, and Sichuan provinces. Salmonella isolates were serotyped and tested for antimicrobial susceptibility. Of the 300 chicken carcasses, 43.3% were positive for Salmonella, with an overall mean of 1.7 log MPN per carcass (95% confidence interval, 1.6 to 1.8 log MPN per carcass). No significant differences (P > 0.05) were detected for storage temperature (i.e., chilled, frozen, or ambient), market type (large, small, or wet), province, or location (capital or noncapital city). Seventy-eight serotypes were identified among the 1,094 Salmonella isolates. The top five most common Salmonella serotypes on raw chicken carcasses were Enteritidis (19.2%), Indiana (15.2%), Typhimurium (14.6%), Agona (7.1%), and Thompson (6.6%). Salmonella isolates (n = 779) were most frequently resistant to sulfisoxazole (74.1%) and tetracycline (71.1%) and least resistant to ceftriaxone (22.5%) and cefoxitin (19%). Only 4% of the isolates were susceptible to all 15 antimicrobial agents, 45% were resistant to 1 to 5 agents, 29% were resistant to 6 to 10 agents, and 22% were resistant to 11 to 15 agents. Our findings revealed that Salmonella contamination was common in retail raw poultry in China, and the counts on contaminated carcasses were mostly low. Salmonella isolates were diverse in their serotype distribution and antimicrobial susceptibility profiles, with more than half of the isolates resistant to more than five antimicrobial agents. These data may be used in risk assessment models to reduce the transmission of Salmonella via chicken meat to humans in China.

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Section: Discussionsupporting

confidence: 93%

Counts, Serotypes, and Antimicrobial Resistance of Salmonella Isolates on Retail Raw Poultry in the People’s Republic of China

Yang

Cui

Shi

et al. 2014

Journal of Food Protection

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“…These results were similar to those of other investigators. [30][31][32] Our study indicated that Salmonella isolates were most frequently resistant to tetracycline (77.12%), sulfisoxazole (66.62%), and nalidixic acid (64.74%), and this was consistent with the findings of other studies. 33,34 This study presented that 92.92% of the class I integron-positive isolates were resistant to at least two antibiotics, and this exhibited a good evidence for the relationship between the presence of class I integron and the emerging of MDR in gram-negative bacteria.…”

Section: Discussionsupporting

confidence: 90%

Antibiotic Susceptibility and Molecular Screening of Class I Integron in Salmonella Isolates Recovered from Retail Raw Chicken Carcasses in China

Meng

Zhang

et al. 2017

Microbial Drug Resistance

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Salmonella is one of the leading causes for foodborne diseases. Foods, particularly those of animal origin, act as an important role for Salmonella transmission. In this study, the antibiotic susceptibility of 743 Salmonella isolates recovered from retail raw chicken carcasses in eight provinces was tested, and the isolates were also screened for the presence of class I integron and drug-resistant gene cassettes. One hundred thirteen (15.21%) isolates were harboring class I integron. A higher percentage of integron-positive Salmonella isolates were found in retail chicken in Sichuan Province (29.33%), followed by Beijing (22.14%), Shaanxi (19.15%), Guangxi (14.13%), Henan (12.50%), Shanghai (7.25%), Fujian (8.22%), and Guangdong (6.25%) Provinces. The respective prevalence of class I integron in Salmonella isolates recovered from retail chickens in large, free, and small markets was 16.31%, 14.04%, and 15.27%. Moreover, 20.13%, 14.02%, and 13.74% of Salmonella isolates recovered from retail chickens stored in frozen, chilled, and ambient conditions, respectively, were positive for class I integron. Subsequent sequencing of class I integron revealed the presence of 10 gene cassettes harboring resistance genes (dfrA17-aadA5, dfrA17-aadA5, dfrA1-aadA1, dfrA12-aadA2, dfrA17-aadA5-aadA4, dfrA1-aadA1-aadA2, dfrA1, dfrA5, aadA2, aacA4-catB8-aadA1-dfrA1-(aac6-II)-(bla-8), bla-bla). The most prevalent gene cassette was dfrA17-aadA5 (59.62%). Class I integron-positive isolates were significantly more resistant to multiple antibiotics, and they commonly exhibited corresponding antibiotic resistance profiles to the antibiotic resistance gene cassettes harbored in their class I integron. The results indicated that class I integron with different antibiotic resistance gene cassettes that were prevalent in Salmonella isolates differed from provinces, marketplaces, and chicken storage conditions.

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“…In the present study, the mean concentration of all samples approached 0.524 MPN/g, and ranged from 0.013 to 14.50 MPN/g. In a Cambodian study, a very high level of contamination was found at 3 to 4 logCFU/g for positive samples (Lay, Vuthy, Song, Phol, & Sarthou, 2011).…”

Section: Discussionmentioning

confidence: 97%

Quantitative surveys of Salmonella and Campylobacter on retail raw chicken in Yangzhou, China

et al. 2016

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Prevalence, Numbers and Antimicrobial Susceptibilities of Salmonella Serovars and Campylobacter spp. in Retail Poultry in Phnom Penh, Cambodia

Cited by 55 publications

References 17 publications

Counts, Serotypes, and Antimicrobial Resistance of Salmonella Isolates on Retail Raw Poultry in the People’s Republic of China

Counts, Serotypes, and Antimicrobial Resistance of Salmonella Isolates on Retail Raw Poultry in the People’s Republic of China

Antibiotic Susceptibility and Molecular Screening of Class I Integron in Salmonella Isolates Recovered from Retail Raw Chicken Carcasses in China

Quantitative surveys of Salmonella and Campylobacter on retail raw chicken in Yangzhou, China

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