Prevalence of Antimicrobial Resistant and Extended-Spectrum Beta-Lactamase-producing <i>Escherichia coli</i> in Dairy Cattle Farms in East Tennessee

Gelalcha, Benti Deresa; Ensermu, Desta Beyene; Agga, Getahun E.; Vancuren, Molly; Gillespie, B. E.; D’Souza, Doris H.; Okafor, Chika C.; Dego, Oudessa Kerro

doi:10.1089/fpd.2021.0101

Cited by 16 publications

(20 citation statements)

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“…On each sampling occasion, composite soil cores and composite cow faeces from cow pats in a recently grazed paddock, farm dairy effluent (FDE) and bulk tank milk (referred to hereafter as milk) were collected ( Supplementary Table 2 ). Previous studies looking at the prevalence of antimicrobial resistant E. coli in soil from dairy farms have also utilised a sampling strategy which focuses on critical source areas where a large number of dairy cows are concentrated or areas recently impacted by dairy cows, such as housing areas, gateways, recently grazed paddocks and manure fertilised soil ( Liu et al, 2019 ; Gelalcha et al, 2022 ). The FDE management strategy on Dairy 1 changed during the study period; initially effluent was filtered before being administered to paddocks, but later effluent was disposed of via the municipal waste-water system and as a result, two FDE sample collection points were used ( Supplementary Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

et al. 2022

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Antimicrobial resistance (AMR) is a global threat to human and animal health, with the misuse and overuse of antimicrobials being suggested as the main driver of resistance. In a global context, New Zealand (NZ) is a relatively low user of antimicrobials in animal production. However, the role antimicrobial usage on pasture-based dairy farms, such as those in NZ, plays in driving the spread of AMR within the dairy farm environment remains equivocal. Culture-based methods were used to determine the prevalence and distribution of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli from farm environmental samples collected over a 15-month period from two NZ dairy farms with contrasting management practices. Whole genome sequencing was utilised to understand the genomic epidemiology and antimicrobial resistance gene repertoire of a subset of third-generation cephalosporin resistant E. coli isolated in this study. There was a low sample level prevalence of ESBL-producing E. coli (faeces 1.7%; farm dairy effluent, 6.7% from Dairy 4 and none from Dairy 1) but AmpC-producing E. coli were more frequently isolated across both farms (faeces 3.3% and 8.3%; farm dairy effluent 38.4%, 6.7% from Dairy 1 and Dairy 4, respectively). ESBL- and AmpC-producing E. coli were isolated from faeces and farm dairy effluent in spring and summer, during months with varying levels of antimicrobial use, but no ESBL- or AmpC-producing E. coli were isolated from bulk tank milk or soil from recently grazed paddocks. Hybrid assemblies using short- and long-read sequence data from a subset of ESBL- and AmpC-producing E. coli enabled the assembly and annotation of nine plasmids from six E. coli, including one plasmid co-harbouring 12 antimicrobial resistance genes. ESBL-producing E. coli were infrequently identified from faeces and farm dairy effluent on the two NZ dairy farms, suggesting they are present at a low prevalence on these farms. Plasmids harbouring several antimicrobial resistance genes were identified, and bacteria carrying such plasmids are a concern for both animal and public health. AMR is a burden for human, animal and environmental health and requires a holistic “One Health” approach to address.

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Section: Methodsmentioning

confidence: 99%

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

et al. 2022

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“…Our published and ongoing studies also showed an increased prevalence of ESBLs-Ent (e.g., E. coli , Klebsiella spp.) from dairy manure and bulk tank milk [ 18 ].…”

Section: Use Of Beta-lactam Antibiotics In the Usa Dairy Cattle Farmsmentioning

confidence: 99%

“…Some studies reported the worldwide distribution of bla CTX-M-15 gene variants of ESBL is due to its frequent association with IncF plasmids [ 24 , 38 , 95 , 104 ]. Molecular epidemiological studies have shown that these plasmids frequently harbor additional genes involved in fitness, virulence, and other ARGs (e.g., qnr , qepA , tetA , floR , sul2 , and cmlA ) that may help the host bacteria to survive and thrive in human and animal hosts [ 18 , 96 , 105 , 106 , 107 ]. E. coli ST131 is the classic example of the most successful ESBLs-producing pathogenic strains associated with IncF plasmids.…”

Section: Molecular Epidemiology Of Extended-spectrum Beta-lactamases ...mentioning

confidence: 99%

“…Molecular studies have consistently shown that ESBL genes in Enterobacteriaceae isolates are physically associated with MGEs that carry other resistance genes mediating resistance to an unrelated class of antibiotics [ 110 , 111 ]. Co-resistance to multiple classes of antibiotics such as fluoroquinolones, tetracyclines, chloramphenicol, aminoglycosides, sulfonamides, and /or trimethoprim has been frequently reported in E. coli and Salmonella species isolated from dairy cattle and other food-producing animals (e.g., in Pigs and poultry) in the USA and elsewhere (e.g., India, China, and Egypt [ 18 , 24 , 111 , 112 , 113 , 114 , 115 ].…”

Section: Molecular Epidemiology Of Extended-spectrum Beta-lactamases ...mentioning

confidence: 99%

“…Ceftiofur, a 3GC, is one of the top three most frequently used antibiotics to treat and prevent mastitis and other diseases of dairy cattle [ 5 , 17 ]. Recent studies indicated that resistance to beta-lactam antibiotics, specifically resistance to 3GCs, is rising among commensal Enterobacteriaceae isolates from the USA dairy cattle [ 18 ]. Resistance to 3GCs is mainly mediated by the production of ESBLs, a group of enzymes that break down a beta-lactam ring of the extended-spectrum cephalosporins such as 3GCs [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Extended-Spectrum Beta-Lactamases Producing Enterobacteriaceae in the USA Dairy Cattle Farms and Implications for Public Health

Gelalcha

Dego

2022

Antibiotics

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Antimicrobial resistance (AMR) is one of the top global health threats of the 21th century. Recent studies are increasingly reporting the rise in extended-spectrum beta-lactamases producing Enterobacteriaceae (ESBLs-Ent) in dairy cattle and humans in the USA. The causes of the increased prevalence of ESBLs-Ent infections in humans and commensal ESBLs-Ent in dairy cattle farms are mostly unknown. However, the extensive use of beta-lactam antibiotics, especially third-generation cephalosporins (3GCs) in dairy farms and human health, can be implicated as a major driver for the rise in ESBLs-Ent. The rise in ESBLs-Ent, particularly ESBLs-Escherichia coli and ESBLs-Klebsiella species in the USA dairy cattle is not only an animal health issue but also a serious public health concern. The ESBLs-E. coli and -Klebsiella spp. can be transmitted to humans through direct contact with carrier animals or indirectly through the food chain or via the environment. The USA Centers for Disease Control and Prevention reports also showed continuous increase in community-associated human infections caused by ESBLs-Ent. Some studies attributed the elevated prevalence of ESBLs-Ent infections in humans to the frequent use of 3GCs in dairy farms. However, the status of ESBLs-Ent in dairy cattle and their contribution to human infections caused by ESBLs-producing enteric bacteria in the USA is the subject of further study. The aims of this review are to give in-depth insights into the status of ESBL-Ent in the USA dairy farms and its implication for public health and to highlight some critical research gaps that need to be addressed.

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Beta-lactamase genes in bacteria from food animals, retail meat, and human surveillance programs in the United States from 2002 to 2021

Rahman,

Rodriguez-Mori,

Loneragan

et al. 2024

Comparative Immunology, Microbiology and Infectious Diseases

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Prevalence of Antimicrobial Resistant and Extended-Spectrum Beta-Lactamase-producing Escherichia coli in Dairy Cattle Farms in East Tennessee

Cited by 16 publications

References 54 publications

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

Extended-Spectrum Beta-Lactamases Producing Enterobacteriaceae in the USA Dairy Cattle Farms and Implications for Public Health

Beta-lactamase genes in bacteria from food animals, retail meat, and human surveillance programs in the United States from 2002 to 2021

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