Prevalence of antimicrobial-resistant Cutibacterium isolates and development of multiplex PCR method for Cutibacterium species identification

Koizumi, Juri; Nakase, Keisuke; Hayashi, Nobuyuki; Nasu, Yutaka; Hirai, Yuji; Nakaminami, Hidemasa

doi:10.1016/j.jiac.2022.10.018

Cited by 5 publications

(8 citation statements)

References 43 publications

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“…In Japan, Koizumi et al described variable resistance rates according to species. Cutibacterium avidum was the most resistant species, with a resistance rate of 25%, which is consistent with other studies on Cutibacterium species [ 90 , 91 ]. As Actinomyces spp.…”

Section: Clindamycinsupporting

confidence: 91%

Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates

et al. 2023

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Anaerobic bacteria are normal inhabitants of the human commensal microbiota and play an important role in various human infections. Tedious and time-consuming, antibiotic susceptibility testing is not routinely performed in all clinical microbiology laboratories, despite the increase in antibiotic resistance among clinically relevant anaerobes since the 1990s. β-lactam and metronidazole are the key molecules in the management of anaerobic infections, to the detriment of clindamycin. β-lactam resistance is usually mediated by the production of β-lactamases. Metronidazole resistance remains uncommon, complex, and not fully elucidated, while metronidazole inactivation appears to be a key mechanism. The use of clindamycin, a broad-spectrum anti-anaerobic agent, is becoming problematic due to the increase in resistance rate in all anaerobic bacteria, mainly mediated by Erm-type rRNA methylases. Second-line anti-anaerobes are fluoroquinolones, tetracyclines, chloramphenicol, and linezolid. This review aims to describe the up-to-date evolution of antibiotic resistance, give an overview, and understand the main mechanisms of resistance in a wide range of anaerobes.

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Section: Clindamycinsupporting

confidence: 91%

Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates

et al. 2023

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“…Cutibacterium strains were collected from 212 patients with acne who visited Toranomon Hospital between 2013 and 2018, and 264 patients who visited 13 dermatology clinics between 2016 and 2017 ( 4 , 12 , 28 , 29 ). The colonies grown from specimens isolated from the patients under anaerobic conditions were determined using multiplex PCR for the identification of Cutibacterium species, according to our previous report ( 29 ). The strains could not be determined using multiplex PCR but were determined by BLAST homology analysis of 16S rRNA sequences.…”

Section: Methodsmentioning

confidence: 99%

Multidrug Resistance Plasmid pTZC1 Could Be Pooled among Cutibacterium Strains on the Skin Surface

et al. 2023

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The emergence of antimicrobial resistance not only in Cutibacterium acnes strain but also other skin bacteria such as Staphylococcus epidermidis is a big concern due to antimicrobial use for the treatment of acne vulgaris. Increased prevalence of macrolides-clindamycin resistant C. acnes relates to the acquisition of exogenous antimicrobial resistance genes. erm (50) is harbored by the multidrug resistance plasmid pTZC1, which has been found in C. acnes and C. granulosum strains isolated from patients with acne vulgaris.

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“… C. avidum TP-CV302 was isolated from an acne patient in 2016 ( 7 ). The study was approved by the Research Ethics Committee of Tokyo University of Pharmacy and Life Sciences (approval number 16-21).…”

Section: Announcementmentioning

confidence: 99%

“…The acne specimen was collected with a sterilized swab and cultured on modified Gifu anaerobic agar medium (Nissui Pharmaceutical, Tokyo, Japan) at 35°C under anaerobic conditions using the AnaeroPack-Anaero (Mitsubishi Gas Chemical Company, Inc., Tokyo, Japan). Cutibacterium species were identified using a multiplex PCR that was developed previously ( 7 ). The genomic DNA of C. avidum TP-CV302 was extracted using phenol-chloroform-isoamyl alcohol (25:24:1) as reported previously ( 8 ).…”

Section: Announcementmentioning

confidence: 99%

Emergence of Cutibacterium avidum with erm (X) on a Mobile Genetic Element Identical to That of Cutibacterium acnes

Koizumi

Nakase

Nakaminami

2023

Microbiol Resour Announc

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We determined that the Cutibacterium avidum isolate TP-CV302, from a patient with acne vulgaris in Japan, had the macrolide-clindamycin resistance factor erm (X) located on Tn 5432 . Although this mobile genetic element (MGE) is well recognized in Cutibacterium acnes , it has not been found in Cutibacterium avidum .

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Prevalence of antimicrobial-resistant Cutibacterium isolates and development of multiplex PCR method for Cutibacterium species identification

Cited by 5 publications

References 43 publications

Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates

Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates

Multidrug Resistance Plasmid pTZC1 Could Be Pooled among Cutibacterium Strains on the Skin Surface

Emergence of Cutibacterium avidum with erm (X) on a Mobile Genetic Element Identical to That of Cutibacterium acnes

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