Prevalence of apoptosis and inner cell allocation in bovine embryos cultured under different oxygen tensions with or without cysteine addition

Soom, Ann Van; Yuan, Yeqing; Peelman, Luc; Matos, D.G. de; Dewulf, Jeroen; Laevens, Hans; Kruif, Aart de

doi:10.1016/s0093-691x(01)00726-9

Cited by 105 publications

(80 citation statements)

References 42 publications

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“…14% embryo cells; Van Soom et al, 2002;Vandaele et al, 2006), but higher than others (around 2% to 3%; Goovaerts et al, 2011). We observed that the in vitro culture of a reduced number of embryos impaired blastocyst production and also quality, in particular by increasing the apoptotic rate per blastocyst.…”

Section: Discussionmentioning

confidence: 53%

“…Moreover, Van Soom et al (1997) observed that timing of blastocyst formation was linked with cell numbers per blastocyst, detecting that blastocysts at D8 had lower numbers of cells than blastocysts at D6 or D7. In turn, the apoptotic cell index increased significantly in blastocysts at D9 in comparison with the previous days (Van Soom et al, 2002). Vandaele et al (2006) also observed significantly higher apoptosis rates in blastocysts at D8 than at D7.…”

Section: Discussionmentioning

confidence: 80%

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Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 80%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…Abundant evidence presented over the last decade indicates that developmental potential of embryos in vitro is mainly dependent on oocyte quality from which the embryo originates (Sirard et al 2003;Rodriguez and Farin 2004) and the culture environment to which embryos are exposed (Lonergan et al 1999;Van Soom et al 2002;Yuan et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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“…This may be a special requirement for fast proliferating growing embryos; something similar observed with cancer cells [25]. Culturing oocytes and embryos at below ambient O 2 levels have been shown to be favorable for embryo development in various mammalian species including human [2,26,27]. Moreover the glucose level has been found to be an important factor under different oxygen concentrations in the culture environment affecting oocyte and embryo development in vitro [5].…”

Section: Discussionmentioning

confidence: 97%

“…Studies in the past had shown that mammalian oocytes/ embryos develop better under reduced oxygen tension compared to atmospheric oxygen in vitro resulting in higher blastocyst formation rates, less apoptotic cells and more inner cell mass cell numbers [1][2][3]. The oxidative stress on oocytes / embryos had been shown to be directly proportional to their metabolism status where the aerobic metabolism results in the production of co-factors which impact the cellular redox status [4].…”

Section: Introductionmentioning

confidence: 99%

Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition

Kumar

Verma²,

Kumar³

et al. 2015

J Assist Reprod Genet

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Purpose This study evaluates the effect of low oxygen conditions (5 Vs 20 %) on buffalo embryo development. Expression patterns of key glucose metabolism genes (HK, PFK, LDH, PDH, G6PDH and Glut1) were assessed in buffalo oocytes and embryos cultured at 5 and 20 % oxygen and correlated with development rate. Methods Maturation rate was observed by determining MII stages by Aceto-orcein method and blastocyst formation was observed at 7 day post insemination (dpi). Expression levels of genes were determined by real time PCR in oocytes / embryos at 5 and 20 % O 2 .Results Oocyte maturation and blastocyst formation rates were significantly higher at 5 % O 2 as compared to 20 % O 2 (P<0.05). The expression pattern of glycolytic genes (HK, PFK and G6PDH) indicated that oocytes and embryos under 5 % O 2 tend to follow anaerobic glycolysis and pentose phosphate pathways to support optimum embryo development. Under 20 % O 2 , oocytes and embryos had high expression of PDH indicating higher oxidative phosphorylation. Further, less G6PDH expression at 20 % O 2 was indicative of lower pentose phosphate activity. Higher expression of LDH was observed in oocytes and embryos under 20 % O 2 indicating sub-optimal culture conditions. High Glut1 activity was observed in the oocytes / embryos at 5 % O 2 , indicative of high glucose uptake correlating with high expression of glycolytic genes. Conclusion The expression patterns of glucose metabolism genes could be a valuable indicator of the development potential of oocytes and embryos. The study indicates the importance of reduced oxygen conditions for production of good quality embryos.

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Prevalence of apoptosis and inner cell allocation in bovine embryos cultured under different oxygen tensions with or without cysteine addition

Cited by 105 publications

References 42 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition

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