Prevalence of Arcobacter spp. on chicken meat at retail markets and in farm chickens in Selangor, Malaysia

Amare, L.B.; Saleha, A. A.; Zunita, Z.; Abu, Jalila; Hassan, Latiffah

doi:10.1016/j.foodcont.2010.11.004

Cited by 27 publications

(27 citation statements)

References 42 publications

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“…As multiplex PCR for Arcobacter species was widely used in previous studies (23,26,32,33), the primers ARCO (5=-CCT GGA CTT GAC ATA GTA AGA ATG A-3=), BUTZ (5=-CGT ATT CAC CGT AGC ATA GC-3=), SKIR (5=-GGC GAT TTA CTG GAA CAC A-3=), CRY1 (5=-TGC TGG AGC GGA TAG AAG TA-3=), and CRY2 (5=-AAC AAC CTA CGT CCT TCG AC-3=) were used to detect Arcobacter species in this study. The multiplex PCR mixture contained 1 mM concentrations of deoxynucleoside triphosphate s (dNTPs) (Bioneer), 2 l of 10ϫ reaction buffer (Tris [pH 9.0], 15 mM MgCl 2 ) (Bioneer), a 1.25 M concentration of each primer, 0.05 U/l Top polymerase (Bioneer), and 2 l of DNA template, with deionized water added to bring the total reaction volume to 20 l. Multiplex PCR was performed on an MJ mini personal thermal cycler (Bio-Rad, Mexico) with the following conditions: initial denaturation at 94°C for 5 min, followed by 35 cycles of denaturation at 94°C for 45 s, annealing at 61°C for 45 s, extension at 72°C for 45 s, and final extension at 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

et al. 2014

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This study aimed to develop a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Arcobacter species. Specific primers targeting the 23S ribosomal RNA gene were used to detect Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii. The specificity of the LAMP primer set was assessed using DNA samples from a panel of Arcobacter and Campylobacter species, and the sensitivity was determined using serial dilutions of Arcobacter species cultures. LAMP showed a 10-to 1,000-fold-higher sensitivity than multiplex PCR, with a detection limit of 2 to 20 CFU per reaction in vitro. Whereas multiplex PCR showed cross-reactivity with Campylobacter species, the LAMP method developed in this study was more sensitive and reliable than conventional PCR or multiplex PCR for the detection of Arcobacter species.

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Section: Methodsmentioning

confidence: 99%

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

et al. 2014

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“…Among these species, A. butzleri, A. cryaerophilus, A. skirrowii and A. cibarius are considered as emerging food-borne pathogens (Ho et al 2006a;Prouzet-Mauleon et al 2006;Snelling et al 2006;Houf & Stephan 2007;Collado et al 2009a;Amare et al 2011;McGrego & Wright 2015). During the recent years, Arcobacter spp.…”

Section: Evolution and Emergence Of Arcobactersmentioning

confidence: 99%

“…Arcobacters have been reported worldwide from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries like Belgium, United States of America, Denmark, Brazil, Australia, Italy, the Netherlands, Malaysia, Japan, Spain, Czech Republic, Korea, Egypt and India (Lehner et al 2005;Atabay et al 2006;Ho et al 2006a;Snelling et al 2006;Pejchalova et al 2008;Collado et al 2010;Lee et al 2010;Vilar et al 2010;Amare et al 2011;Gonz alez & Ferr us 2011;Patyal 2011;Suelam 2012;Ramees et al 2014aRamees et al , 2014bRamees et al , 2014cMohan et al 2014;Gilbert et al 2014). Arcobacter is a potential food-and water-borne pathogen, and thus pose serious public health concerns (Gonz alez et al 2007b;Gugliandolo et al 2008;Miller et al 2009;Ferreira et al 2016).…”

Section: Epidemiology and Transmission In Animalsmentioning

confidence: 99%

“…Poultry act as potential reservoirs for Arcobacters and these organisms have been recovered from live poultry skin, droppings and meat (Corry & Atabay 2001;Kabeya et al 2004;Lehner et al 2005;Ho et al 2008b;Collado et al 2009a;Amare et al 2011;Ramees et al 2014a;Rahimi et al 2014). A study by Giacometti et al (2015) indicated absence of Arcobacter spp.…”

Section: Epidemiology and Transmission In Animalsmentioning

confidence: 99%

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Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

138

130

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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“…Many of these species have been isolated especially from poultry (Amare et al 2011;Atabay et al 1998), meat (Collado et al 2009a, b;Lee et al 2010;Ohlendorf and Murano 2002), faeces (Van Driessche et al 2005) and from aborted cattle foetuses. Recently, however, they are very often found also in samples from marine environments (Collado et al 2009a, b;Levican et al 2012 including two new described species, Arcobacter ebronensis and Arcobacter aquimarinus (Levican et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Modified isolation method of Arcobacter spp. from different environmental and food samples

2015

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This study provides information on the occurrence of Arcobacter in several types of water and food products of animal origin in the Czech Republic. We processed 190 samples using the modified method, and the occurrence of Arcobacter spp. was confirmed in 36.8 % of these. This total incidence consisted of Arcobacter butzleri (27.3 %), Arcobacter cryaerophilus (8.4 %) and Arcobacter skirrowii (1.1 %). We newly described the common presence of Arcobacter spp. in sewage water in the Czech Republic that is released into waterways after processing in water treatment plants (86.7 %). All the acquired isolates were subject to detailed confirmation with subsequent species classification using multiplex PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we used a modification of a method using passive filtration of an enriched sample, which could be suitable for the isolation of Arcobacter, especially in combination with Campylobacter selective agar chromogenic medium. Our studies have shown this agar to be quite suited to the isolation of Arcobacter and that it can be an appropriate instrument for accelerating culture diagnostics.

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Prevalence of Arcobacter spp. on chicken meat at retail markets and in farm chickens in Selangor, Malaysia

Cited by 27 publications

References 42 publications

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Modified isolation method of Arcobacter spp. from different environmental and food samples

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