Background and Aim: Physical inactivity may cause poor physical fitness, obesity, cardio-respiratory and mental illness. The present study was designed to find out the effects of short term yoga practice on body composition, physical fitness, physiological variables, nutrition and mental status of school children of 10 to 12 years. Materials and Methods: For the present study 120 volunteers (60 healthy female and 60 healthy male) within the age group of 10–12 years were screened randomly and were divided into: (a) Yoga Group (n = 60) and (b) Control Group (n = 60). Both these groups were sub-divided into (i) Male (n = 30) and (ii) Female (n = 30) volunteers. The volunteers of yoga group followed a yoga practice schedule of 1hr/day, 6 days/week for 6 weeks, with no yoga practice in control group. Body composition, physical fitness, physiological variables, nutrition and mental status were measured before (0 week) and after (6 weeks) training. Results: A significant increase (p ≤ 0.05) in strength, flexibility, anaerobic power, VO2max, FVC, FEV1, PEFR; and decrease (p ≤ 0.05) in resting heart rate, blood pressure, depression, anxiety and stress scores were observed in both male and female volunteers after six weeks of yoga practice. Conclusion: Regular practice of yogic asana, pranayama, and meditation improves body composition, cardiovascular, respiratory, physical fitness, and reduces stress level.