Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Nofal, Ayman S.; El-Leboudy, Ahlam A.; El-Makarem, Hussein S. Abo; El-Rahman, Amir H. Abd

doi:10.5455/ajvs.278394

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…These tests are considered more sensitive but less specific than MRT (Aggad & Boukraa, 2006 ), also these tests are less sensitive than the ELISA test, as the sensitivity of direct milk-ELISA is 95% - 96.5% and specificity of 99% - 100% according to (Jalali et al , 2003 ; Kerby et al , 1997 ; Nielsen et al , 1996; Kerkhofs et al , 1990 ; Sutherhand et al , 1986). In contrast, our results lower than that mentioned by (Kang’ethe et al ., 2000 ) in raw milk which ranged from 3.4% to 3.9% with MRT, and 2.4-4.9% with a milk-ELISA test in Kenya, and also lower than that recorded cow’s milk with MRT test (4.0%) by in Azerbaijan (Aliyev et al ., 2022 ), Tunisia (Béjaoui et al ., 2022 ), Egypt (Nofal et al ., 2017 ), and in Algeria (Aggad & Boukraa, 2006 ). No significant difference was observed in the prevalence rate of brucella among the animal by using the Milk-Ring test or by using the Milk-ELISA test.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of <em>Brucella</em> spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen

et al. 2022

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Brucella infection in animals is considered a great problem in most countries of the world. Our study designed to determine the prevalence of brucella in field animal’s milk in Dhamar governorate, Yemen. Total of 808 raw milk samples from non-aborted field animals, 120 milk samples from aborted animals, and 30 pasteurized milk samples were teste by Milk-Ring Test (MRT), milk-ELISA test, isolation and identification of brucella species, and antibiotic susceptibility. The prevalence of brucella in milk samples from field animals was 0.8%, 2.6%, and 2% in cows, sheep, and goat milk samples respectively with MRT, and 0.8%, 1.3% and 1.6% in cows, sheep and goat milk samples respectively with the milk- ELISA test. The prevalence rate in milk samples from aborted animals was 33%, 64% and 41.2% with the MRT and 39%, 49%, and 41.2% in cows, sheep and goats respectively with the milk-ELISA test. All pasteurized milk samples were negative for the milk-ELISA test. The result of isolation showed 0.1% of Brucella in milk samples from field animals while 9.2% from aborted animals. All isolates of Brucella species were sensitivities to rifampicin, doxycycline, kanamycin, gentamicin, streptomycin, tetracycline, and ciprofloxacin, while resistant to ampicillin, erythromycin, and novobiocin. In conclusion, the high prevalence of milk brucella especially in aborted animals needs focusing and build controlling strategies plans to decrease the losses to the economy and avoid transferred to humans with unpasteurized milk consumption.

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Section: Discussionmentioning

confidence: 99%

Prevalence of <em>Brucella</em> spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen

et al. 2022

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“…In populations of cattle and buffalo, B. abortus and B. melitensis are the most commonly recovered species while their biovars are distributed differently between countries (Refai, 2002;Abbas and Talei, 2010;Nofal et al, 2017;Deka et al, 2018;Abera et al, 2019;Almashhadany, 2019). It is recognized that B. abortus has a preference for cattle over other ruminants, while B. melitensis is the common causal agent of brucellosis in sheep and goat.…”

Section: Discussionmentioning

confidence: 99%

Serological and Bacteriological Screening of Brucellosis in Blood and Milk of Cows and Buffaloes

Almashhadany¹

2021

SVU-International Journal of Veterinary Sciences

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Brucellosis remains a serious infection to human and animal populations in developing countries with detrimental effects on public health. The study aimed to evaluate the occurrence of brucellosis in cow and buffaloes at Erbil Governorate, Iraq by detection anti-Brucella antibodies and isolation of Brucella species. A total of 265 blood samples (140 from cows and 125 from buffaloes) were collected from villages around Erbil city, and 320 raw milk samples (170 from cow and 150 from buffaloes) were randomly collected from dairy farms during the period from July to December 2019. The results showed an overall seroprevalence of 11.7% according to rose Bengal test (RBT). The isolation of Brucella abortus was 7/19 (36.8%) and 12 / 19 (63.2%), while 9/24 (37.5%) and 15 /24 (62.5%) were Brucella melitensis from cattle and buffaloes milk samples, respectively. Noticeable increase in occurrence was found in November (20.8%), while the lowest rate was seen in July (5.9%). In conclusion, brucellosis is still a significant public health hazard in Erbil Governorate. Based on the test performance, the study recommends MRT as a rapid screening test for detecting brucellosis in milk in farms, centers, and dairy factories. Consumers are also recommended to adequately pasteurize the milk in order to kill this milk-borne pathogen before consumption.

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“…However, it too amplified all Brucella species as well as O. anthropi . These primers targeting BCSP31 are still used today in diagnostics in the Middle East, Asia, and East Africa [107–110]. However, it does not exclude vaccine strains from detection and was validated only on post-culture colony isolates [111].…”

Section: Diagnosticsmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

152

129

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Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

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Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Cited by 3 publications

References 16 publications

Prevalence of <em>Brucella</em> spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen

Prevalence of <em>Brucella</em> spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen

Serological and Bacteriological Screening of Brucellosis in Blood and Milk of Cows and Buffaloes

Comparisons of brucellosis between human and veterinary medicine

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