Prevalence of class 1 and 2 integrons among the multidrug resistant uropathogenic strains of <i>Escherichia coli</i>

Haddadi, Azam; Ghezeljeh, Somayeh Mikaili; Shavandi, Mahmoud

doi:10.5372/1905-7415.0901.367

Cited by 7 publications

(14 citation statements)

References 15 publications

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“…The frequency of MDR isolates of E. coli from UTIs is high in Iran (10,19). In the present study, the highest resistance rate was seen for ceftriaxone, cefotaxime, and cotrimoxazole, which is consistent with previous reports in Iran (3,20). Moreover, in accordance with previous reports in Iran, all isolates were sensitive to imipenem (2,20,21).…”

Section: Discussionsupporting

confidence: 92%

“…Nowadays, the E. coli resistance to antibiotics used for the UTI treatment has been increased (2). Inappropriate using of antibiotics, as well as the horizontal gene transfer between bacteria, have led to the development of multi-drug resistant (MDR) strains (3). Mobile genetic elements such as plasmids, transposons, and integrons play an important role in the development of antibiotic-resistant strains (4).…”

Section: Introductionmentioning

confidence: 99%

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The Relationship of Class I Integron Gene Cassettes and the Multidrug-Resistance in Extended -Spectrum β-Lactamase Producing Isolates of Escherichia coli

Akya

Lorestani

Rostamian

et al. 2019

Arch Pediatr Infect Dis

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Background: Integron is an important element in creating multi-drug resistant (MDR) bacteria. This study evaluated the relationship of class I integron and antibiotic resistance in extended-spectrum beta-lactamase producing (ESBL) isolates of Escherichia coli (E. coli). Methods: A total of 66 ESBL-producing E. coli were isolated from urinary tract infection in Kermanshah and their antimicrobial susceptibility was assessed. The incidence of class I integron was determined in isolates using PCR. The class I integron-associated gene cassettes were also verified by DNA sequencing. Data were analyzed using statistical methods. Results: Of 66 ESBL-producing isolates, 65 (98.5%) were MDR. The most prevalent antibiotic resistance of the isolates was observed for ampicillin (98.4%), ceftriaxone (98.4%), cefotaxime (95.4%), and co-trimoxazole (86.3%). The frequency of class I integron in isolates was 92.3%. The relationship between class I integron and resistance to streptomycin, co-trimoxazole, and ceftazidime was statistically significant. The genes encoding resistance to streptomycin and co-trimoxazole, as well as a gene encoding a protein with unknown function, were associated with class I integron. The most common gene cassette was dfrA17-aadA5. Conclusions: The results indicate a high prevalence of antibiotic resistance among ESBL-producing isolates to the antibiotics commonly used for the empirical treatment of urinary tract infections. The frequency of class I integron and associated gene cassettes involving resistance to antibiotics is high. There is a high percentage of MDR among the ESBL-producing isolates, which mostly contain gene cassettes. These findings suggest a strong association of integron and ESBL genes in the isolates.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Relationship of Class I Integron Gene Cassettes and the Multidrug-Resistance in Extended -Spectrum β-Lactamase Producing Isolates of Escherichia coli

Akya

Lorestani

Rostamian

et al. 2019

Arch Pediatr Infect Dis

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“…In addition, results of this study showed that 65.7% (23/35) of the multidrug resistant isolates had integron genes. This result is in contrast to previously published report [9], which showed that 30% of the multidrug resistant isolates harbored integrons. However, in other study [10], reported that all MDR isolates were carried integrons.…”

Section: Discussioncontrasting

confidence: 99%

“…In current study, all E. coli isolates that had integron genes were carried only class 1 integron and none other tested classes. This result is in contrast to other reports previously published, which showed that the class 1 and 2 integrons were detected in E. coli isolates [9,[29][30][31]. However, this result is in agreement with other report previously published, which showed that the class 1 integron was the only type detected among clinical E. coli isolates [8].…”

Section: Discussionsupporting

confidence: 77%

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Determination of Antibiotic Resistance Profile and Virulence Genes in Escherichia coli Isolates from Palestinian Patients

Adwan

2019

JAMB

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Introduction: Escherichia coli (E. coli) is considered one of the most frequent intestinal and extraintestinal pathogen. Methods: A total of 49 isolates of E. coli were collected from different clinical samples, from different hospitals in Northern West Bank-Palestine, during January-March 2019. Aims: To detect the distribution of Type III secretion system (T3SS) genes, class 1, 2 and 3 integrons, virulence factors (fyuA, papGIII, iutA and sfa⁄foc) using multiplex PCR and antibiotic resistance using disc diffusion method. Results: In this study, E. coli isolates showed high resistance rate against different types of antibiotics and 71.4% of the isolates were multidrug resistant (MDR). Only class 1 integron was detected in these isolates with prevalence 57%, and 65.7% of MDR isolates carried integron genes. The prevalence of T3SS genes was 0.0%. In addition, results of this study showed that the prevalence of virulence genes papGIII, sfa⁄foc, fyuA and iutA was 4.1%, 40%, 64%, and 79.6%, respectively. Conclusions: The isolates of E. coli showed high resistance rate against different types of antibiotics. The co-occurrence of class 1 integrons and antimicrobial resistance genes in these isolates is an additional threat for spread of the antimicrobial resistance traits which may further complicate future strategies for treatment the infections caused by this pathogen. In addition, E. coli isolated from Palestinian patients showed one or more virulence factors that could increase their pathogenic potential.

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Risk of sharing resistant bacteria and/or resistance elements between dogs and their owners

2022

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Background The indiscriminate use and the similarity of prescribed antibiotics especially beta-lactams in human and small animal medicine, along with the close communication between pets and humans, increases the risk of the transfer of antibiotic-resistant bacteria and/or resistance elements especially integrons, between them. Therefore, we aimed to compare the frequencies of extended spectrum beta-lactamase (ESBL)-producing strains, major ESBL genes, classes 1 and 2 integrons, and antibiotic resistance patterns of fecal Escherichia coli (E. coli) isolates from dogs and their owners. Methods The present study was conducted on 144 commensal E. coli isolates from the feces of 28 healthy dog-owner pairs and 16 healthy humans who did not own pets. Phenotypic confirmatory test was used to identify the frequencies of ESBL-producing E. coli. Frequencies of blaCTX-M, blaSHV, and blaTEM genes, and also classes 1 and 2 integrons were determined by polymerase chain reaction. Resistance against 16 conventional antibiotics was determined by disk diffusion technique. Results ESBL-production status was similar between the E. coli isolates of 71.4% of dog-owner pairs. The E. coli isolates of 75, 60.7, and 85.7% of dog-owner pairs were similar in terms of the presence or absence of blaCTX-M, blaTEM, and blaSHV genes, respectively. The presence or absence of class 1 and class 2 integrons was the same in E. coli isolates of 57.1% of dog-owner pairs. Prevalence of resistance to chloramphenicol and tetracycline was significantly higher in E. coli isolates of dogs than owners, but for other 10 (83.3%) tested antibiotics, no statistically significant difference was found in prevalence of antibiotic resistance between dogs and owners isolates. Furthermore, the antibiotic-resistance profile was the same in the E. coli isolates of 14.3% of dog-owner pairs. Conclusions The results of current research highlight the seriousness of the drug-resistance problem and the need to prevent further increases and spread of antibiotic-resistance to reduce treatment failure. Moreover, relatively similar characteristics of the E. coli isolates of dogs and their owners can show the risk of sharing resistant bacteria and/or resistance elements between them.

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Prevalence of class 1 and 2 integrons among the multidrug resistant uropathogenic strains of Escherichia coli

Cited by 7 publications

References 15 publications

The Relationship of Class I Integron Gene Cassettes and the Multidrug-Resistance in Extended -Spectrum β-Lactamase Producing Isolates of Escherichia coli

The Relationship of Class I Integron Gene Cassettes and the Multidrug-Resistance in Extended -Spectrum β-Lactamase Producing Isolates of Escherichia coli

Determination of Antibiotic Resistance Profile and Virulence Genes in Escherichia coli Isolates from Palestinian Patients

Risk of sharing resistant bacteria and/or resistance elements between dogs and their owners

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