Prevalence of Clostridium perfringens in Retail Meat and Meat Products with Some Decontamination Trials by some Essential Oils

El-Bayomi, Rasha M.; El-Mesalamy, Yasmen M.; Hafez, Abd-Elsalam E.; Ahmed, Heba A.

doi:10.17582/journal.jahp/2020/9.s1.61.68

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…The bacterial colonies were used to prepare slide and were stained by Grams' staining to know the characteristics of bacteria. Biochemical assessments were performed to determine the species of bacterial isolates on the basis of their biochemical characteristics (El-Bayomi et al, 2020;Musawa et al, 2021). Then antibiotic sensitivity test was performed to assess the sensitivity of pathogenic organism against eight different antimicrobials i.e., norfloxacin, tetracycline, neomycin, gentamicin, ampicillin, streptomycin, ciprofloxacin and erythromycin.…”

Section: Bacteriological Examinationmentioning

confidence: 99%

Evaluation of Bacterial Contamination From Raw and Cooked Fish, Mutton and Beef Sold by Local Vendors in Hyderabad, Pakistan

Ansari¹,

Abro²,

Tanweer³

et al. 2022

JAHP

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Foodborne pathogens are spreading to humans through contamination of raw and cooked meat because of its inappropriate processing, handling and cooking. Therefore this research was performed for the evaluation of bacterial contamination from raw and cooked fish, mutton and beef sold by retailors in district Hyderabad. During present study, 150 total meat samples, 100 from raw meat (beef=40, mutton=40, fish=20) and 50 from cooked meat (beef=20, mutton=20, fish=10) were randomly collected from district Hyderabad and cultured on different media for isolation of bacterial species. The isolated species were identified by different biochemical tests. The results showed that the contamination of bacterial organisms in both raw and cooked meat was highest in beef followed by mutton and fish respectively (p < 0.05). In raw meat, bacterial species recorded were Escherichia coli (45%, 30% and 25%), Salmonella enteritidis (20%, 17.5% and 15%), Staphylococcus aureus (30%, 25% and 25%), Bacillus cereus (12.5%, 10% and 10%), Klebsiella pneumoniae (15%, 10% and 0%) and Shigella dysenteriae (10%, 12.5% and 5%) in beef, mutton and fish respectively. While from cooked beef, mutton and fish the prevalence of E. coli (25%, 25% and 20%), S. aureus (15%, 15% and 10%), S. enteritidis and B. cereus (10%, 10% and 10%) were observed. The highest (p < 0.05) bacterial load (g ¹) was detected in raw (1.76×10 6 ) and cooked (6.1×10 4 ) beef than raw (1.55×10 6 ) and cooked (4.5×10 4 ) mutton respectively, while raw (1.25×10 6 ) and cooked (2.9×10 4 ) fish exhibited the least (p < 0.05) bacterial load than other raw and cooked meat (beef and mutton) respectively. Data regarding antimicrobial susceptibility exhibited that among eight antibiotics E. coli, S. enteritidis, S. aureus and B. cereus were observed sensitive to gentamycin, norfloxacin and ciprofloxacin. K. pneumoniae showed sensitivity against gentamycin, norfloxacin, ciprofloxacin, erythromycin, tetracycline and streptomycin; whereas S. dysenteriae was observed sensitive to gentamycin, norfloxacin, tetracycline and ampicillin. In conclusion, raw beef samples were found more contaminated than raw fish and mutton while cooked fish samples were observed less contaminated than cooked beef and mutton. Furthermore, all bacterial isolates (except K. pneumoniae) were found multidrug resistant.

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Section: Bacteriological Examinationmentioning

confidence: 99%

Evaluation of Bacterial Contamination From Raw and Cooked Fish, Mutton and Beef Sold by Local Vendors in Hyderabad, Pakistan

Ansari¹,

Abro²,

Tanweer³

et al. 2022

JAHP

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“…Abd El-Tawab et al (2017) reported that the sulfamethoxazoletrimethoprim combination showed moderate efficacy against C. perfringens isolates. El-Bayomi et al (2020) demonstrated that all C. perfringens isolates examined were resistant to oxytetracycline, while 90% of the isolates were resistant to amoxicillin and 80% to ampicillin.…”

Section: Discussionmentioning

confidence: 99%

Role of Clostridium Perfringens and Escherichia Coli in the Occurrence of Diarrhea in newborn Rabbits

2022

Int J Vet Sci

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This study aimed to identify some of the bacterial causes of enteritis and death following post weaning diarrhea in rabbits. A total of 230 samples from diarrheic rabbits, including 70 rectal swabs, 100 samples of livers and intestines (50 each) from freshly dead rabbits and 60 food and water samples (30 each) were taken from rabbits of various breeds from various farms and smallholders in El-Beheira governorate, Egypt. These samples were subjected to bacteriological examination. Molecular characterization was performed to confirm the C. perfringens and E. coli isolates and to detect some virulent genes associated with their pathogenicity. The results showed that the prevalence of C. perfringens in rectal swabs, liver and intestinal samples was 28.5, 44.0 and 84.0%; and the prevalence of E. coli was 74.2, 50.0 and 70.0%, respectively. In addition, examination of 30 water and 30 feeds samples from rabbits’ environment revealed that, the prevalence of C. perfringens was 2/30 and 5/30 while the prevalence of E. coli was 10/30 and 7/30, respectively. E. coli isolates were serogrouped into O153, O125, O27, O158 and O148 serogroup. All C. perfringens isolates were C. perfringens type A (had only alpha toxin), while beta and epsilon toxins were not detected. In E. coli, virulent genes (eaeA and tsh genes) were detected in 3 isolates, while the cnf1 gene was not detected in any isolate. Antimicrobial susceptibility testing of most of the isolates indicated the presence of multidrug resistant strains.

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“…Definite toxin types produce intestinal disease in numerous species of animals (Nazki et al, 2017). A study reported the 14.5% occurrence of C. perfringens in food samples and recommended that it is important to ensure product protection mainly when it is to be consumed by newborns and elderly people (El-Bayomi et al, 2020). A previous study conducted in Punjab, Pakistan showed 64 and 37% prevalence of C. perfringens in diseased and healthy animals respectively (Mohiuddin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Clostridium perfringens in Small Ruminants of District Kalat, Balochistan

Hakeem,

Kamboh,

Matar

et al. 2024

IJAR

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Background: Among life-threatening infections of small ruminants (sheep/goat), enterotoxaemia has vital importance. This disease is caused by certain serotype of Clostridium perfringens and commonly known as pulpy kidney or overeating infection. The current study was carried out to determine the prevalence of C. perfringens in small ruminants in different zones (Kalat, Mangochar, Johan, and Gazg) of district Kalat, Balochistan. Methods: Fecal samples (n=100) were collected aseptically, from rectum of enterotoxaemia suspected animals. The samples were cultured in Reinforced Clostridial Media (RCM). Biochemically identified isolates were further confirmed through polymerase chain reaction (PCR). Result: Overall prevalence of C. perfringens was noticed 48%, with higher occurrence rate (p less than 0.01) in sheep (60%) than goat (36%). Among fifty samples of each specie, 30 and 18 isolates recovered from sheep and goat respectively. The area wise analysis showed that, in goats, the highest prevalence was found in Kalat (50%) followed by the Mangochar (38.46%), Johan (30.77%) and Gazg (25%). The statistical analysis revealed the significant differences (p less than 0.05; 95% confidence interval (CI) 18.80-68.91) in C. perfringens prevalence in various areas of Kalat district. The results of the gender-wise prevalence of C. perfringens exhibited a numerically higher (p greater than 0.05; 95% CI 9.23-49.02) prevalence in female goats (40%) than male goats (32%). Whereas, the age-wise analysis indicated that, in less than 1 year age group (44.44%), the prevalence of C. perfringens was higher (p less than 0.05; 95% CI 8.00-57.89) than greater than 1 but less than 2 years (29.41%) and ³ 2-year (33.33%) age groups. Similarly, in sheep, the highest prevalence (p less than 0.05) was found in Kalat (76.92%) followed by Mangochar (66.67%), Johan (58.33%) and Gazg (38.46%). The age wise and gender-wise differences (p less than 0.05) were also observed in various age and gender groups of sheep similar to goat. Among 30 sheep isolates of C. perfringens, a highest number (28 isolates) showed resistance against amikacin, followed by amoxicillin/penicillin (25 isolates) and colistin sulphate (22 isolates); while among 18 isolates of goat 17 were found resistant against colistin sulphate and 16 were resistant against amoxicillin and amikacin. In conclusion enterotoxaemia is prevailing in small ruminants of district Kalat, Balochistan thus mass scale vaccination campaign should be carried out to prevent losses.

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Prevalence of Clostridium perfringens in Retail Meat and Meat Products with Some Decontamination Trials by some Essential Oils

Cited by 3 publications

References 6 publications

Evaluation of Bacterial Contamination From Raw and Cooked Fish, Mutton and Beef Sold by Local Vendors in Hyderabad, Pakistan

Evaluation of Bacterial Contamination From Raw and Cooked Fish, Mutton and Beef Sold by Local Vendors in Hyderabad, Pakistan

Role of Clostridium Perfringens and Escherichia Coli in the Occurrence of Diarrhea in newborn Rabbits

Prevalence and Antibiogram of Clostridium perfringens in Small Ruminants of District Kalat, Balochistan

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