Prevalence of Clostridium perfringens type A isolates in different tissues of broiler chickens

Doosti, Abbas; Pasand, M.; Mokhtari-Garsani, A.; Ahmadi, Reza; Chechelgerdi, M.

doi:10.15547/bjvm.919

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Multiplex PCR technique showed that all ten isolates in this study harboured cpa gene which give characteristic bands at 400 bp confirming that all of C. perfringens type. This result goes hand in hand with several anthers [42,43,44].…”

Section: Discussionmentioning

confidence: 70%

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Ibrahim¹

2017

AVS

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Clostridium perfringens organisms have an economic concern in poultry production. The purpose of this study was to investigate Net B and β 2 C. perfringens positive isolates in broiler farms and their clinic-pathological effects in broiler chicks. A bacteriological examination of C. perfringens was carried upon 92 Necrotic enteritis (NE) diseased cases and 55 apparently healthy broilers of different ages in Egypt. C. perfringens type A was only recovered (49.7%). cpa gene was detected in 100% of samples with PCR technique. NE diseased cases exhibited both Net B (87.5%) and cpb 2 (75%) toxin genes. Experimentally, an intra-gut induction of Net B and β 2 C. perfringens toxins were evaluated in chicken models. The hematological studies revealed hemolytic anemia 5 days post infection (p.i) in Net B and β 2 inoculated groups (G1&G2). Leucogram revealed neutrophilia and lymphopenia 5 days p.i. A significant increase in ALT, AST, uric acid and creatinine serum levels were recorded in the infected groups at 5 th and 12 th day p.i. DNA Sequencing for net B gene revealed an amino acid replacement from glutamate into arginine at codon 379 with silent mutation was also detected at nucleotide 1134. Sequencing of both toxin genes were recorded in the gene bank for the first time in Egypt. This study pointed out that C. perfringens Net B toxin, is a new key virulent factor for the development of NE. Further studies of Net B toxiod for vaccine production could minimize the clostridial problems in broiler farms.

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Section: Discussionmentioning

confidence: 70%

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Ibrahim¹

2017

AVS

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Clostridium perfringensContamination in Retail Meat and Meat-Based Products in Bursa, Turkey

Yibar

Çetin

Ata³

et al. 2018

Foodborne Pathogens and Disease

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This study examined the incidence of Clostridium perfringens in raw, ready-to-cook (RTC), and ready-to-eat (RTE) meat and meat-based products (N = 306) collected from restaurants, supermarkets, and butcher shops in Bursa, Turkey. In addition, we investigated the presence of the C. perfringens enterotoxin (CPE), as well as cpe genes and their source (chromosomal or plasmid borne). In this study, tryptose sulfite cycloserine (TSC) agar for classic culture isolation and API and real-time polymerase chain reaction (RT-PCR) techniques were used to identify C. perfringens and detect cpa and cpe genes from these products, respectively. Seventeen C. perfringens isolates (5.6%) were isolated and identified with API 20A. In addition, 42 of 81 suspicious isolates (51.9%) were identified as C. perfringens using RT-PCR. Of the 81 suspicious isolates tested by RT-PCR, 22 (27.2%) carried the cpe gene either on the plasmid or chromosome. Twenty-one isolates were positive for chromosomal cpe (C-cpe), and one was positive for plasmid-borne cpe (P-cpe). CPE was detected in 31.8% (7/22) of the cpe positive isolates by the PET-RPLA test. In conclusion, C. perfringens and their CPEs were present in raw, RTC, and RTE meat and meat-based foods in this study. It is emphasized that the presence of C. perfringens and the cpe gene in these foods may be a potential risk for human health.

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Clostridium Perfringens Toxin Types Associated with Meat: Review in Iran

Masoumi¹,

Hosseini²,

Moghaddam³

et al. 2021

Iran J Med Microbiol

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Food poisoning due to Clostridium perfringens (C. perfringens) is a major food health problem, particularly in terms of meat consumption. Due to human's susceptibility to this pathogen, detection methods and prevention measures should be implemented to reduce its incidence. Several pathogenic strains of C. perfringens have been identified so far. One of the potential concerns about this bacterium is its toxin-producing characteristic that causes food poisoning. It has seven toxin types (A-G) according to the existence of four unique toxin genes. This study aimed to assess the prevalence of food poisoning caused by C. perfringens in meat and meat-derived products in Iran. We collected and categorized all the available data on this issue in Iran. Moreover, we summarized some methods used to detect toxins and genes and finally placed a prevention section for clarifying how to prevent such events. The best method for preventing such an organism's growth is by keeping foods in their normal state (hot and cold criteria) and chilling prepared foods in shallow containers as soon as possible.

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Prevalence of Clostridium perfringens type A isolates in different tissues of broiler chickens

Cited by 3 publications

References 17 publications

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Clostridium perfringensContamination in Retail Meat and Meat-Based Products in Bursa, Turkey

Clostridium Perfringens Toxin Types Associated with Meat: Review in Iran

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Prevalence of Clostridium perfringens type A isolates in different tissues of broiler chickens

Cited by 3 publications

References 17 publications

Toxin Genotyping of &lt;i&gt;C. perfringens&lt;/i&gt; Isolated from Broiler Cases of Necrotic Enteritis

Toxin Genotyping of &lt;i&gt;C. perfringens&lt;/i&gt; Isolated from Broiler Cases of Necrotic Enteritis

Clostridium perfringensContamination in Retail Meat and Meat-Based Products in Bursa, Turkey

Clostridium Perfringens Toxin Types Associated with Meat: Review in Iran

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Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis

Toxin Genotyping of <i>C. perfringens</i> Isolated from Broiler Cases of Necrotic Enteritis