Prevalence of colistin resistance gene mcr-1 in Escherichia coli isolated from chickens in central China, 2014 to 2019

Zhang, Wenting; Zhāng, Téngfēi; Wang, Chen; Liang, G.C.; Qin, Lü; Wen, Guoyuan; Guo, Yunqing; Cheng, Ye; Wang, Zui; Shao, Hongxia; Luo, Qingping

doi:10.1016/j.jgar.2022.03.024

Cited by 15 publications

(15 citation statements)

References 26 publications

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“…Our results confirmed that mcr -positive isolates were resistant to other antibiotics [ 10 , 25 , 26 ]. Except for one isolate, all mcr -positive strains were MDR and 56.1% of them were resistant to ciprofloxacin.…”

Section: Discussionsupporting

confidence: 85%

“…Up to the present, seven additional mcr genes ( mcr-4 to mcr-10 ) have been identified [ 6 ], with mcr-1 remaining the most prevalent [ 7 ]. The most common plasmids carrying the mcr-1 gene, which are IncI2, IncHI2 and IncX4, have been shown to be highly transferable and to carry other resistance genes [ 8 , 9 , 10 ]. They contributed to the widespread distribution of the mcr-1 gene worldwide [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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High Fecal Prevalence of mcr-Positive Escherichia coli in Veal Calves at Slaughter in France

Dupouy

Arpaillange

et al. 2022

Antibiotics

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The aim of this study was to determine the percentage of healthy veal calves carrying mcr-positive E. coli strains at the time of slaughter in France. Fecal samples were selectively screened for mcr-positive E. coli isolates using media supplemented with colistin. Screening for mcr genes was also carried out in E. coli isolates resistant to critically important antimicrobials used in human medicine recovered from the same fecal samples. Overall, 28 (16.5%) out of the 170 veal calves tested carried mcr-positive E. coli. As some calves carried several non-redundant mcr-positive strains, 41 mcr-positive E. coli were recovered. Thirty-one and seven strains were positive for mcr-1 and mcr-3 genes, respectively, while no strain was positive for the mcr-2 gene. Co-carriage of mcr-1 and mcr-3 was identified in three strains. All mcr-positive E. coli isolates, except one, were multidrug-resistant, with 56.1% being ciprofloxacin-resistant and 31.7% harboring blaCTX-M genes. All mcr-3-positive E. coli carried blaCTX-M genes, mainly blaCTX-M-55. This study highlights the high prevalence of mcr-positive E. coli strains in feces of veal calves at the time of slaughter. It also points out the multidrug (including ciprofloxacin) resistance of such strains and the co-occurrence of mcr-3 genes with blaCTX-M-55 genes.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

High Fecal Prevalence of mcr-Positive Escherichia coli in Veal Calves at Slaughter in France

Dupouy

Arpaillange

et al. 2022

Antibiotics

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“…The incidence rate of colistin resistance among poultry birds was reported as 6.6% in Bangladesh [ 23 ]. In a study from China, the rate of colistin resistance among E. coli isolates from poultry farms increased from 4.1% (2014) to 16.2% (2019) [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Escherichia coli Coharboring mcr-1 and Extended Spectrum Beta-Lactamases from Poultry

Jamil

Zahoor

Nawaz

et al. 2022

BioMed Research International

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Background. The emergence of resistance to beta-lactam agents in poultry results in multidrug-resistant (MDR) phenotypes in Escherichia coli isolates from poultry birds. The appearance of mobile colistin resistance (mcr) genes in the poultry sector has further worsened the situation. Therefore, the current study is aimed at investigating the molecular epidemiology of mcr harboring colistin-resistant E. coli among poultry. Methods. The isolation and identification of colistin-resistant E. coli (CR-Ec) were done from the broiler’s fecal samples through culturing using selective media supplemented with colistin sulfate (4 μg/ml). The antibiogram studies of the isolates were performed using the disc diffusion method and broth microdilution method as per CLSI guidelines. The screening for the genes conferring resistance to colistin as well as beta-lactam agents was performed by PCR. The genetic diversity of mcr-positive strains was assessed by multilocus sequencing typing (MLST). Results. Out of 500 fecal samples, 7% (35/500) were found positive for the presence of colistin-resistant E. coli (CR-Ec). Among the CR-Ec isolates, 74.28% (26/35) were detected as ESBL producers carrying the blaCTX-M-1 gene in 15/35 (42.85%) isolates and blaCTX-M-15 and blaTEM genes in 21/35 (60%) and 35/35 (100%) isolates, respectively. E. coli isolates were found positive for the presence of mcr-1, although none of the isolates exhibited the mcr-2 or mcr-3 genes. The MLST of CR-Ec has shown the ST1035 as the most prevalent genotype, while 82.85% (29/35) of CR-Ec strains belonged to clonal complex (CC) 131 comprising ST1035, ST131, ST1215, ST1650, and ST2279. Conclusions. The findings suggest a continuous monitoring system in veterinary and clinical settings to avoid unnecessary antibiotics. Further studies are needed at the national level to help control the increasing resistance among Enterobacterales in poultry settings.

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“…Since the discovery of mcr-1 as a plasmid-mediated determinant of mucin resistance in China, the study of this gene has received increasing attention from all over the world [9,17,19]. Therefore, the mcr-1 gene is usually considered to be the main mechanism of polymyxin resistance, especially in the Enterobacteriaceae [20,21], but this has been reported mainly in Escherichia coli and less frequently in K. pneumoniae [22].…”

Section: Discussionmentioning

confidence: 99%

“…Although, plasmid-mediated colistin-resistance genes from mcr-1 to mcr-10 encoding the phosphor-ethanolamine transferase has been widely reported worldwide [8], the colistin-resistant Enterobacteriaceae were mostly found in Escherichia coli [9][10]. And the reports of the mgrB gene alter were primary mechanism of colistin resistant in K. pneumoniae [11][12].…”

Section: Introductionmentioning

confidence: 99%

MgrB mutations causing colistin resistance in ST11 and ST15 Klebsiella pneumoniaes in a tertiary teaching hospital in China

zhao

Song

Guo

et al. 2022

Preprint

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Introduction:Colistin-resistant carbapenem-resistant Klebsiella pneumoniae (ColR-CRKP) is a threat to public health safety. The main cause of polymyxin resistance is a change in the negative charge and L-Ara-N and PEtN molecules on the cell membrane, which leads to a decrease in the affinity of polymyxin for the strain. Methodology: In this study, (ColR-CRKP) isolates and clinical data were collected from a tertiary teaching hospital in China .Testing of antimicrobial susceptibility explained on the European Committee on Antimicrobial Susceptibility Testing criteria (EUCAST), Mutations and expression of drug-resistant genes were detected by PCR and RT-PCR. Genotyping of isolates analysised by MLST and PFGE. Results:Fourteen ColR-CRKP strains were collected and all isolates were resistant to colistin and most other clinical antibiotics (except tigecycline and cotrimoxazole), and were divided into 6 different PFGE clusters, and most strains clustered in B,C,D groups. Base mutations in the mgrB gene that can lead to changes in amino acid sequence were found in all 14 strains. Missense mutations a55→t translated into S32C were found in 85.7% isolates (n=12; KP1-9, KP11-12 and KP14). The expression level of mgrB gene was decreased in all ColR-CRKP strains. Conclusions:Third-generation cephalosporins and enzyme inhibitors, as well as carbapenems, may be causative for the mutation. The new challenge for the treatment of ColR-CRKP requires broader attention.Our study showed that mutations in the mgrB gene can lead to the development of polymyxin resistance in in ST11 and ST15-KPC-2-producing K. pneumoniae in this region.

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Prevalence of colistin resistance gene mcr-1 in Escherichia coli isolated from chickens in central China, 2014 to 2019

Cited by 15 publications

References 26 publications

High Fecal Prevalence of mcr-Positive Escherichia coli in Veal Calves at Slaughter in France

High Fecal Prevalence of mcr-Positive Escherichia coli in Veal Calves at Slaughter in France

Genetic Diversity of Escherichia coli Coharboring mcr-1 and Extended Spectrum Beta-Lactamases from Poultry

MgrB mutations causing colistin resistance in ST11 and ST15 Klebsiella pneumoniaes in a tertiary teaching hospital in China

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