“…Current approaches to detect different forms of Fe in saliva include chemiluminescence, ICP-MS, solid-phase ELISA, and different biochemical analyzers. [19][20][21][22][23] These methods are sensitive, specic, and precise to detect low concentrations of analyte. However, most of them are time-consuming and require extensive equipment and trained operators, making it difficult for point-of-care diagnostic testing.…”