Prevalence of Corynebacterium ulcerans in dogs in Osaka, Japan

Katsukawa, Chihiro; Komiya, Takako; Yamagishi, Hiroaki; Ishii, Atsushi; Nishino, Shunji; Nagahama, Shinya; Iwaki, Masaaki; Yamamoto, Akihiko; Takahashi, Motohide

doi:10.1099/jmm.0.034868-0

Cited by 38 publications

(42 citation statements)

References 25 publications

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“…It is now the most common cause of diphtheria in the UK (Wagner et al, 2010). Recent literature also suggests that C. ulcerans isolated from domestic pigs (Schuhegger et al, 2009), domestic cats (De Zoysa et al, 2005) and dogs (Lartigue et al, 2005;Katsukawa et al, 2009) may be a reservoir for human infection. We have previously described C. ulcerans from a case of mastitis in a bonnet macaque and as a frequent contaminant of cephalic implants from macaques used in cognitive neuroscience (Fox & Frost, 1974;Bergin et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Corynebacterium species in macaques

Venezia

Cassiday

Marini

et al. 2012

Journal of Medical Microbiology

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Bacteria of the genus Corynebacterium are important primary and opportunistic pathogens. Many are zoonotic agents. In this report, phenotypic (API Coryne analysis), genetic (rpoB and 16S rRNA gene sequencing), and physical methods (MS) were used to distinguish the closely related diphtheroid species Corynebacterium ulcerans and Corynebacterium pseudotuberculosis, and to definitively diagnose Corynebacterium renale from cephalic implants of rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques used in cognitive neuroscience research. Throat and cephalic implant cultures yielded 85 isolates from 43 macaques. Identification by API Coryne yielded C. ulcerans (n574), Corynebacterium pseudotuberculosis (n52), C. renale or most closely related to C. renale (n53), and commensals and opportunists (n56). The two isolates identified as C. pseudotuberculosis by API Coryne required genetic and MS analysis for accurate characterization as C. ulcerans. Of three isolates identified as C. renale by 16S rRNA gene sequencing, only one could be confirmed as such by API Coryne, rpoB gene sequencing and MS. This study emphasizes the importance of adjunct methods in identification of coryneforms and is the first isolation of C. renale from cephalic implants in macaques.

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Section: Introductionmentioning

confidence: 99%

Characterization of Corynebacterium species in macaques

Venezia

Cassiday

Marini

et al. 2012

Journal of Medical Microbiology

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“…Although the differential diagnosis was sarcoma, the rapid formation of the mass strongly suggested an infectious lesion rather than a malignant tumour. Because human infection with this bacterium has been frequently reported (Bonmarin et al, 2009;Katsukawa et al, 2012), the incidence of subcutaneous lesions due to C. ulcerans may increase in the near future. Therefore, the fact that C. ulcerans can cause a subcutaneous painful mass in the extremities is important for differential diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…Using PFGE (De Zoysa et al, 1995), the isolate was genetically compared with four isolates that were representatives of four groups into which a previous study classified the Japanese strains (Katsukawa et al, 2012). The location cities from which the four C. ulcerans isolates had been reported were Chiba (strain 0102) (Hatanaka et al, 2003;Komiya et al, 2010), Okayama (strain 0509) (Asakura et al, 2006), Oita (strain 0510) (Nureki et al, 2007) and Kanagawa (strain 0607) (Hagiwara et al, 2006).…”

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confidence: 99%

Subcutaneous abscess formation in the upper extremity caused by toxigenic Corynebacterium ulcerans

Urakawa

Seto

Yamamoto

et al. 2013

Journal of Medical Microbiology

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Corynebacterium ulcerans is attracting attention as an emerging zoonosis that causes lymphadenitis, dermatitis and respiratory infections. We report here what appears to be the first case of subcutaneous abscess formation in the upper extremity due to toxigenic C. ulcerans in Japan. Awareness of the fact that C. ulcerans can cause a subcutaneous, elastic-hard, lessmobile mass with heat, redness and pain in the extremities is important for differential diagnosis. IntroductionCorynebacterium ulcerans is known as an emerging zoonosis that causes cutaneous infection, sinusitis, tonsillitis, pharyngitis, pneumonia and peritonitis (Dias et al., 2011). However, to the best of our knowledge, C. ulcerans has not been reported to cause subcutaneous abscess in the extremities. Therefore, clinicians encountering a subcutaneous mass in the extremities are unlikely to consider subcutaneous abscesses due to C. ulcerans as a differential diagnosis. This could lead to a delay in diagnosis and treatment, and in the worst scenario, it may result in a fatal diphtheria-like disease (Mattos-Guaraldi et al., 2008;Putong et al., 2011).Here, we report the diagnosis and treatment of a patient with a subcutaneous abscess in the upper extremity due to C. ulcerans and discuss the clinical characteristics useful for differential diagnosis. Case reportIn December 2011, a 37-year-old Japanese woman was referred to Tsuruoka Municipal Shonai Hospital, in Tsuruoka, Japan, with a painful mass in her right elbow. The patient had no cardiac symptoms. Her past medical history was unremarkable. Three days before the current hospital visit, she experienced swelling and itching on the medial side of her right elbow. Within a few days, a hot, red, painful mass rapidly formed subcutaneously.Physical examination revealed redness and local heat on the surface of the skin over the mass. The mass was approximately 4 cm in size, elastic hard and less mobile, and caused pain and tenderness. No radiating pain was produced on tapping on the mass (Tinel's sign). Laboratory tests revealed an increase in C-reactive protein levels (2.3 mg dl 21) and a white blood cell count in the normal range (7500 mm 23 ). Radiography did not reveal calcification within the lesion. On magnetic resonance imaging, a subcutaneous soft tissue mass (27622626 mm in size) was observed on the medial side of her elbow; it exhibited iso-signal intensity on T1-weighted images (Fig. 1a) and inhomogeneous high-signal intensity on T2-weighted images (Fig. 1b). Following intravenous administration of gadopentetate dimeglumine, ring enhancement was seen on T1-weighted images (Fig. 1c).Fine-needle aspiration biopsy was performed. No pus was aspirated, but small fragments of tissue were obtained. The tissue fragments were sent for pathological analysis and microbiological diagnosis. Histopathological examination showed no malignancy, but neutrophils and histiocytes were present in the specimen. Bacterial cultures were negative under aerobic conditions. In January 2012, the patient underwent surgery...

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“…Moreover, toxigenic Corynebacterium ulcerans strains were isolated from domestic dogs in Japan. Comparative analysis of the isolates using PFGE and toxin gene typing suggests that transmission between asymptomatic dogs might have occurred (Katsukawa et al 2009(Katsukawa et al , 2012. On the other hand, Corynebacterium diphtheriae has been isolated from dairy cattle (Greathead and Bisschop 1963), a cow with chronic active dermatitis (Corboz et al 1996), and domestic cats with severe otitis (Hall et al 2010).…”

Section: Ecologymentioning

confidence: 97%