Prevalence of Coxiella Burnetii Infection in Cattle, Black Bengal Goats and Ticks in Bangladesh

Chakrabartty, A.; Bhattacharjee, P. K.; Sarker, Roma Rani; Rahman, Abdur; Henning, Klaus; Neubauer, H.; Rahman, Md. Shafiqur

doi:10.3329/bjvm.v14i1.28827

Cited by 12 publications

(7 citation statements)

References 27 publications

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“…Animals may be shedding bacteria and/or remain seropositive long after the acute infection [ 39 , 40 ]. Other authors reported significantly higher seroprevalence in goats than in cattle [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

High prevalence of Coxiella burnetii infection in humans and livestock in Assiut, Egypt: A serological and molecular survey

Abbass

Selim

Sobhy³

et al. 2020

Vet World

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Background and Aim: Q fever is considered a neglected zoonotic disease and is caused by Coxiella burnetii. Very little information is available on C. burnetii infections in cattle, sheep, and goat populations in Egypt. The aim of this study was to identify the seroprevalence of C. burnetii in humans and livestock and to test for the presence of C. burnetii DNA in sera from seropositive animals and humans. Materials and Methods: Blood samples were collected from 160 apparently healthy farm animals and 120 patients from three hospitals of the Assiut Governorate throughout 2017/2018. These populations were tested for antibodies against C. burnetii phase II antigen by immunofluorescence assay [IFA]) and enzyme-linked immunosorbent assay (ELISA). Seropositive samples were subjected to real-time quantitative polymerase chain reaction (RT-qPCR). Results: The results of the IFA revealed C. burnetii seroprevalence rates of 45.3%, 56.0%, 45.7%, and 53.3% in cattle, sheep, goats, and humans, respectively. In humans, the seroprevalence rates were 52.1%, 30.4%, 37.5%, 74.1%, and 62.5% in patients with fever of unknown origin, influenza, kidney dialysis, hepatitis C virus, and hepatitis B virus, respectively. Likewise, by ELISA, the seroprevalence in bovine was 50.7%; sheep, 60.0%; goats, 51.4%; and humans, 55.0% (54.3%, 30.4%, 37.5%, 77.8%, and 62.5% in patients with fever of unknown origin, influenza, kidney dialysis, hepatitis C virus, and hepatitis B virus, respectively). RT-qPCR targeting the repetitive element IS1111 confirmed the presence of C. burnetii DNA. Conclusion: These results proved that apparently healthy cattle, sheep, and goats may be very important reservoirs of C. burnetii infection. In light of these data, the effect of Q fever on the replication of hepatitis virus remains unclear. Although hepatitis is one of the main aspects of acute Q fever, the influence of hepatitis on Q fever remains to be investigated. Q fever is not a reportable disease in Egypt, and clinical cases may rarely be recognized by the health-care system. Additional information on the epidemiology of C. burnetii in Egypt is warranted, including other associated problems such as the distribution of infections, pathologic hallmarks, and molecular typing.

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Section: Discussionmentioning

confidence: 99%

High prevalence of Coxiella burnetii infection in humans and livestock in Assiut, Egypt: A serological and molecular survey

Abbass

Selim

Sobhy³

et al. 2020

Vet World

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“…A standard curve was generated using decimal dilutions of the positive control. The PCR primer sequences and conditions were applied as described by Chakrabartty et al 2016 [25].…”

Section: Methodsmentioning

confidence: 99%

Serological and Molecular Investigation of Coxiella burnetii in Small Ruminants and Ticks in Punjab, Pakistan

Ullah

El-Adawy

Jamil

et al. 2019

IJERPH

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Coxiellosis is a zoonotic disease caused by the obligate intracellular bacterium Coxiella burnetii affecting the productive and reproductive capabilities of animals. This study was conducted to gain insight into the seroprevalence of coxiellosis in small ruminants in seven farms of the Punjab, Pakistan. Potential risk factors were assessed. In total, 1000 serum samples (500 from sheep and 500 from goats) and 163 ticks were collected from the ruminants. All these 163 ticks were merged into 55 pools (29 pools for ticks from sheep and 26 pools for ticks from goat). Serum samples were investigated using an indirect ELISA and PCR. Coxiella burnetii DNA was detected in 29 pooled seropositive samples and 11 pooled ticks by real-time qPCR. Serological analysis revealed a prevalence of 15.6% and 15.0% in sheep and goats, respectively. A significant association was found between seropositivity and different variables like district, lactational status, reproductive status, body condition and reproductive disorders. Univariate analysis showed that detection of C. burnetii DNA in tick pools was significantly associated with the presence of ticks on sheep and goats. However, a non-significant association was found for the prevalence of C. burnetii DNA in serum pools. Hence, C. burnetii infection is prevalent in small ruminants and ticks maintained at livestock farms in Punjab, Pakistan.

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“…According to a recent report by Chakarabarthy et al , the overall seroprevalence of coxiellosis in Bangladesh was 7.6% and 6.1% for goats and cattle, respectively. However, none of seropositive samples were positive for C. burnetii DNA in real-time PCR [ 35 ]. Two researchers observed that C. burnetii appears to be quite frequent in blood as well as milk products of cattle [ 14 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, none of seropositive samples were positive for C. burnetii DNA in real-time PCR [ 35 ]. Two researchers observed that C. burnetii appears to be quite frequent in blood as well as milk products of cattle [ 14 , 35 ]. Kim et al .…”

Section: Resultsmentioning

confidence: 99%

“…In the recent times, coxiellosis in animals have been reported from several countries such as Bangladesh, Iran, Brazil, Turkey, USA, Greece, Bulgaria, Switzerland, Italy, and The Netherlands [ 14 , 35 , 36 ]. An outbreak of Q fever in Danish goat, leading to killing of 51,680 infected goats and reports of coxiellosis in different countries across the globe have raised the awareness level of Q fever throughout the world [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

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Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Pradeep¹,

Spellman²,

Pratheesh³

et al. 2017

Vet World

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Background and Aim::In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). In this preliminary study, we have proceeded to look for C. burnetii DNA in those antibody positive specimens employing an imported commercial C. burnetii polymerase chain reaction (PCR) kit.Materials and Methods::Blood samples were collected during slaughtering. All 15 blood samples of antibody positive ruminants and three antibody negative samples were subjected to conventional Trans-PCR assay with a commercial PCR kit (Genekam Biotechnology AG, Duisburg, Germany). An in-house Trans-PCR was included in the study for comparison.Results::A total of 15 antibody positive and three antibody-negative serum samples belonging to 11 goat, 4 sheep, 1 cattle, and 2 buffaloes were tested in duplicate for the presence of C. burnetii DNA by the commercial agar gel PCR kit and an in-house Trans-PCR. Only one buffalo serum sample was positive for C. burnetii with a band at 243 bp in in-house Trans-PCR.Discussion::Seropositivity for C. burnetii need not necessarily translate into infectivity status of the animal. Conversely, seronegative ruminants can shed C. burnetii. Rapid disintegration of C. burnetii DNA during the storage period is an important impediment in QF-PCR research. This is the first time the performance of this commercial PCR kit is being validated in India.Conclusion::Commercial PCR kit, Genekam did not identify any positive sample, probably because it targeted a larger amplicon of 687 bp.

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Prevalence of Coxiella Burnetii Infection in Cattle, Black Bengal Goats and Ticks in Bangladesh

Cited by 12 publications

References 27 publications

High prevalence of Coxiella burnetii infection in humans and livestock in Assiut, Egypt: A serological and molecular survey

High prevalence of Coxiella burnetii infection in humans and livestock in Assiut, Egypt: A serological and molecular survey

Serological and Molecular Investigation of Coxiella burnetii in Small Ruminants and Ticks in Punjab, Pakistan

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

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