Prevalence of Cronobacter spp. in various foodstuffs and identification by multiplex PCR

Abstract

Cronobacter spp. are gram-negative, asporogenous, motile bacilli included in the family Enterobacteriaceae. Due to the opportunistic nature of these bacteria they may induce several clinical symptoms in different age groups. High mortality rates were documented especially in the newborns (

Prevalence, phenotypic and genotypic characterization, virulence potential and antimicrobial resistance of Cronobacter species from ready-to-eat foods

“…sakazakii as the most common species among the Cronobacter genus, similar to our result [10,30,38]. Moreover, in many previous studies, the C. malonaticus andother species of Cronobacter were isolated from various foods [4,30,39].…”

“…(51.9%) in meat free cig koftes may be due to contaminated ingredients including cereal (46.7%) and spices (30.8%). In the study of Aksu et al [10] in Turkey, the 50% of the Cronobacter isolates obtained from cig kofte (cereal-based ball) was similar to our research. Four samples of cereals (bulgur, red lentil, peanut, and goji berry) and seven samples of spices (cumin, chili pepper, thyme, mint, flower flour, coconut) were contaminated with Cronobacter spp.…”

“…The result in salads (1/38) was lower than the result (13/30) obtained by Vasconcellos et al [39], while was similar to the result (1/15) obtained by Lee et al [45]. Cronobacter were not detected from dried herbs and vegetables in some studies, similar to our results [10,40]. In another research, the Cronobacter spp.…”

“…was 12.2% in doner, 9.8% in pastramis and 8.6% in kavurmas as retail meat products in this research. Several studies demonstrated varying prevalence of Cronobacter spp.in meat and meat products: 0.0% in Turkey [10], 3.2% in the Netherlands [43], and 14.5% in China [4]. In this study, Cronobacter spp.…”

Emergence of Cronobacter sakazakii in Cases of Neonatal Sepsis in Upper Egypt: First Report in North Africa

“…In Egypt, some herbs like anise, caraway, mint, fenugreek, and chamomile are widely used and are supposed to relieve gastrointestinal disturbances in infants (Al-Nabulsi et al, 2009;John and Shantakumari, 2015). C. sakazakii was detected in 9.2% of the tested herb samples, which is consistent with the results obtained by Aksu et al (2016Aksu et al ( , 2018, who reported in their study that 14% of spice and herb samples tested in Turkey were positive for Cronobacter spp. and that the predominance was for C. sakazakii.…”