Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers

Bang, Dang Duong; Scheutz, Flemming; Ahrens, Peter; Pedersen, Karl; Blom, Jens; Madsen, Mogens

doi:10.1099/0022-1317-50-12-1087

Cited by 75 publications

(55 citation statements)

References 11 publications

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“…The CdtB exerts DNase I-like activity, targeting the eukaryotic DNA, thus triggering a signalling pathway involving several protein kinases, hence resulting in the inhibition of the cell cycle in G2 or M phase [34]. The genes for CDT production (cdtA, cdtB, and cdtC) were detected as 100% in the all isolates tested in this study, which is consistent with previous reports by Bang et al [5] and Asakura et al [3]. However, despite the high prevalence of cdt genes (100%), only 26 strains (31.0%) of C. jejuni and 1 strain (15.3%) of C. coli isolates showed evidence for CDT production in HEp-2 cell cytotoxicity assays.…”

Section: Discussionsupporting

confidence: 82%

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

Cho¹,

Kim²,

Min³

et al. 2014

Korean Journal of Veterinary Research

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: The prevalence of thermophilic Campylobacter (C.) spp. in stray, breeding, and household dogs was 25.2, 12.0, and 8.8%, respectively. C. jejuni and C. upsaliensis were the predominant Campylobacter spp. from household dogs. cdtA, cdtB, and cdtC were detected by PCR in all isolates. Despite the high cytolethal distending toxin (CDT) gene prevalence, only 26 (31%) C. jejuni strains and one (15.3%) C. coli strain showed evidence of CDT production in HEp-2 cell cytotoxicity assays. Virulence-associated genes detected in the C. jejuni and C. coli isolates were cadF, dnaJ, flaA, racR, ciaB, iamA, pldA, virB11, ceuE, and docC. cadF, dnaJ, flaA, and ceuE were found in all C. jejuni and C. coli isolates. When detecting Guillain-Barré syndrome-associated genes (galE, cgtB, and wlaN), galE was identified in all isolates. However, cgtB and wlaN were more prevalent in C. jejuni isolates from humans than those from dogs. Adherence and invasion abilities of the C. jejuni and C. coli strains were tested in INT-407 cells. A considerable correlation (adjusted R 2 = 0.678) existed between adherence and invasion activities of the Campylobacter spp. isolates.

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Section: Discussionsupporting

confidence: 82%

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

Cho¹,

Kim²,

Min³

et al. 2014

Korean Journal of Veterinary Research

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“…When the source of the isolates was included, a higher percentage of C. jejuni (87.5-95.8%) than C. coli (45.8-70.8 %) strains were positive for all cdt genes. The percentage of toxin-positive Campylobacter examined by other authors was from 90% to 100% (Bang et al 2001(Bang et al , 2003Datta et al 2003;Martinez et al 2006). However, little information concerning the prevalence of the cdtA, cdtB and cdtC genes had been published in Poland.…”

Section: Identification Of Putative Virulence Markersmentioning

confidence: 99%

Molecular characterization of Campylobacter spp. isolated from poultry faeces and carcasses in Poland

Wieczorek¹,

Osek²

2011

Acta Vet. Brno

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Campylobacter infection is one of the most common enteric human diseases world-wide but the mechanism of Campylobacter pathogenicity has not been exactly explained yet. One of the main reasons is genotypic, hence phenotypic diversity of the bacterial isolates. The aim of the present study was to perform a molecular characterization of randomly selected C. jejuni and C. coli strains isolated from poultry faeces and carcasses in Poland. Several virulence gene markers were identified by polymerase chain reaction (PCR). Furthermore, genetic typing has also been used by the macrorestriction profiling with pulsed-field gel electrophoresis (PFGE). The results of the present study showed that all analyzed isolates of C. jejuni (n = 24) and C. coli (n = 24) contained the flaA and cadF sequences. On the other hand, the virB11 gene was present only in 6 of 48 (12.5%) of the analyzed isolates, whereas most of the strains contained the cdt genes. Other virulence gene iam linked to Campylobacter invasiveness was present in 34 of 48 (72.9%) strains. The restriction analysis of the whole genome digested with SmaI produced three main clonal groups designed as I, II (with two subgroups IIa and IIb), and III obtained by the comparison of macrorestriction profiling patterns. The results showed a poor correlation between Campylobacter profiles generated by a clonal molecular technique and the presence of virulence markers. Therefore, PCR detection of Campylobacter virulence markers can be utilized as a simple and rapid tool to discriminate stains recovered from different sources, especially when used in conjunction with the PFGE profile analysis as a complex strategy. These kinds of analyses had not been previously carried out in Poland and these results may generate more knowledge regarding the genetic diversity and molecular relationship of Campylobacter.

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“…The latter observation suggests that cytolethal distending toxin may also play a part in the development of the inflammatory process in humans. Recent studies analysing the distribution of separate cdtA, cdtB and cdtC genes or the cdt cluster in C. jejuni and C. coli indicate that their prevalence in isolates from poultry and other sources exceeds 90 % (Bang et al, 2001;Datta et al, 2003). Microarray testing of the cdt cluster showed that these genes were present in all C. jejuni isolates from human samples (Dorrell et al, 2001;Volokhov et al, 2003).…”

Section: ! E Rozynek and Othersmentioning

confidence: 99%

“…The contamination of poultry carcasses in abattoirs is considered a main risk factor for human infection (Bang et al, 2001;Petersen et al, 2001). A recent study in Poland showed that 88 .…”

Section: Introductionmentioning

confidence: 99%

Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses

Rozynek

Dzierżanowska-Fangrat

Jóźwiak

et al. 2005

Journal of Medical Microbiology

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The pathogenicity of thermotolerant Campylobacter species, common food-borne pathogens, depends on certain factors unevenly distributed among strains of different origin. The prevalence of such markers has never been examined in a population of Polish Campylobacter strains of human and poultry origin. Therefore, we analysed the presence of the cadF, cdtA, cdtB and cdtC genes and the iam sequence in Campylobacter jejuni (n ¼ 115) and Campylobacter coli (n ¼ 57) isolates from children with diarrhoea and from chicken carcasses. The cadF gene was present in nearly 100 % of Campylobacter isolates tested, regardless of their origin or species. In contrast, the iam region was found in 83 . 3 % and 100 % of C. coli isolates from children and chickens, respectively, but in only 1 . 6 % and 54 . 7 %, respectively, of C. jejuni isolates. Similarly, the detection rates of cdt genes varied between human and chicken isolates. All three cdt genes were found in nearly all C. jejuni isolates from both children and chickens, but in only 5 . 6 % of human C. coli isolates as compared to 87 . 2 % of chicken C. coli isolates. This different distribution of genetic markers between human and chicken isolates indicates that some Campylobacter infections in children may have additional sources other than contaminated chicken meat.

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Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers

Cited by 75 publications

References 11 publications

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

Molecular characterization of Campylobacter spp. isolated from poultry faeces and carcasses in Poland

Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses

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