Prevalence of different outer membrane proteins in isolates of Aeromonas species

Khushiramani, Rekha; Girisha, S.K.; Bhowmick, Patit Paban; Karunasagar, Indrani

doi:10.1007/s11274-008-9740-4

Cited by 18 publications

(11 citation statements)

References 21 publications

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“…The OMPs of A. hydrophila are rather heterogeneous in most of the strains but a 36 kDa protein is common to most strains. Khushiramani et al (2008) isolated 3 to 4 high-intensity OMP bands from 40 strains of A. hydrophila that ranged from 25 to 45 kDa. Asha et al (2004) studied the expression of antigenic BF from A. hydrophila and identified about 15 protein bands that ranged from 10 to 15 kDa.…”

Section: Discussionmentioning

confidence: 99%

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Viji¹,

Kumari²,

Velmurugan³

et al. 2013

Dis. Aquat. Org.

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Ornamental goldfish Carassius auratus were treated with whole cell (WC), extracellular product (ECP), outer membrane protein (OMP) and biofilm (BF) vaccines developed from the virulent Aeromonas hydrophila (AHV1; GenBank HQ331525.1) with and without the immunoadjuvant Asparagus racemosus. On various days post-vaccination (dpv), the treated fish were challenged with virulent A. hydrophila. These fish were monitored for survival, growth, specific bacterial reduction, and biochemical, haematological and immunological parameters. C. auratus attained 100% mortality within 7 d in non-vaccinated groups, whereas the vaccines helped to significantly (p ≤ 0.001) increase survival after 25 and 50 dpv. The vaccines with immunoadjuvant (ECP 2 , OMP 2 and BF 2 treatments) helped to reduce the Aeromonas load after the challenge, and serum albumin, globulin and protein levels were significantly (p < 0.01) improved in the OMP 2 -and BF 2 -treated groups. Haemoglobin and red blood cell counts were also significantly improved (p < 0.05) in the vaccinated groups compared to the control group. Additionally, haemagglutination occurred at the 1:12 dilution level in the vaccine plus immunoadjuvant-treated groups. Supplementing the vaccines with immunoadjuvant helped to improve phagocytosis to 54.07%, serum bactericidal activity to 14.6% and the albumin:globulin ratio to 7.6% in BF 2 after 50 dpv. Its positive effect significantly (p < 0.05) increased in vaccinated groups compared to controls. Based on the results, especially with the OMP and BF vaccines, the immunoadjuvant A. racemosus helped to improve the efficiency of the vaccines. This approach will aid in the development of more efficient vaccines against bacterial infections affecting the aquaculture industry. KEY WORDS:Ornamental fish · Biofilm · Outer membrane protein · OMP · Herbal immunoadjuvants Resale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 99%

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Viji¹,

Kumari²,

Velmurugan³

et al. 2013

Dis. Aquat. Org.

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“…Omps are reported to be conserved among different serovars. Some of them serve as adhesins and play an important role in virulence [13]. Omps are located at hostebacterial interface in A. hydrophila and can be targeted for drug therapy [13].…”

Section: Introductionmentioning

confidence: 99%

Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu)

Rauta

Nayak

2015

Fish & Shellfish Immunology

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“…For example, Omp48 and OmpW can be used for Aeromonas detection due to their widespread nature and OmpTS for the identification of virulent strains as it is commonly found decorating clinical isolates (Guan et al, 2011;Khushiramani, Girisha, Bhowmick, Karunasagar, & Karunasagar, 2008).…”

Section: Outer-membrane Proteinsmentioning

confidence: 99%

Aeromonas Flagella and Colonisation Mechanisms

Lowry

Balboa

Parker

et al. 2014

Advances in Microbial Physiology

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Prevalence of different outer membrane proteins in isolates of Aeromonas species

Abstract: Outer membrane proteins (Omps) are located at host-bacterial interface and are important for host immune responses and as targets for drug therapy.

Cited by 18 publications

References 21 publications

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Vaccination strategies to protect goldfish Carassius auratus against Aeromonas hydrophila infection

Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu)

Aeromonas Flagella and Colonisation Mechanisms

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