Prevalence of DNA of fourteen human polyomaviruses determined in blood donors

Kamminga, Sergio; Meijden, E. van der; Brouwer, Caroline S. de; Feltkamp, Mariet C.W.; Zaaijer, Hans L.

doi:10.1111/trf.15557

Cited by 25 publications

(38 citation statements)

References 56 publications

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“…PyVs are small, non-enveloped double-stranded DNA viruses. To date, 14 human PyVs have been identified since the discovery of the first two human PyVs, BK virus (BKPyV) and JC virus (JCPyV) 4 – 6 . Patients with BKPyV and JCPyV infection are usually asymptomatic, but immunosuppression can lead to reactivation, which is associated with serious diseases such as BKPyV-induced nephropathy or JCPyV-induced leukoencephalopathy.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and viral loads of polyomaviruses BKPyV, JCPyV, MCPyV, TSPyV and NJPyV and hepatitis viruses HBV, HCV and HEV in HIV-infected patients in China

Zhou

Nakashima

Ito

et al. 2020

Sci Rep

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Human polyomaviruses (PyVs) and hepatitis viruses are often more prevalent or persistent in human immunodeficiency virus (HIV)-infected persons and the associated diseases are more abundant than in immunocompetent individuals. Here, we evaluated seroreactivities and viral loads of human PyVs and hepatitis viruses in HIV/AIDS patients and the general population in China in the combination antiretroviral therapy (cART) era. A total of 810 HIV-1-infected patients and age- and sex-matched HIV-negative individuals were enrolled to assess seroprevalence of PyVs BKPyV, JCPyV, MCPyV, TSPyV, and NJPyV and hepatitis viruses HBV, HCV, and HEV. 583 (72%) patients received cART, and among them, 31.2% had undetectable HIV RNA. While no significant difference was observed in prevalence of anti-PyV antibodies between HIV-positive and -negative groups, serum DNA positivity and DNA copy level of MCPyV were higher in the HIV-positive group. Among HIV-infected patients, BKPyV DNA positivity was significantly higher in patients with CD4 + cell counts < 200 cells/mm3 compared to those with CD4 + cell counts > 500 cells/mm3, suggesting possible reactivation caused by HIV-induced immune suppression. Higher HBV and HCV seropositivities but not HEV seropositivity were also observed in the HIV-positive group. Further correlation analyses demonstrated that HBV and HEV are potential risk factors for increased prevalence of PyV infection.

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Section: Introductionmentioning

confidence: 99%

Prevalence and viral loads of polyomaviruses BKPyV, JCPyV, MCPyV, TSPyV and NJPyV and hepatitis viruses HBV, HCV and HEV in HIV-infected patients in China

Zhou

Nakashima

Ito

et al. 2020

Sci Rep

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“…Recent studies have assessed HPyVs serum antibody levels across a range of age groups including one from the Netherlands. HPyV7 and HPyV6 were found to be highly seroprevalent in all age groups [ 29 , 30 , 31 , 32 , 33 ]. HPyV6 seroprevalence was slightly higher than HPyV7 and MCPyV.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Human Polyomavirus 7 in Cholangiocarcinomas and Adjacent Peritumoral Hepatocytes: Preliminary Findings

et al. 2020

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Cholangiocarcinoma (CCA) is a rare biliary-duct malignancy with poor prognosis. Recently, the presence of the human polyomavirus 6 (HPyV6) has been reported in the bile of diverse hepatobiliary diseases, particularly in the bile of CCA patients. Here, we investigated the presence of novel HPyVs in CCA tissues using diverse molecular techniques to assess a possible role of HPyVs in CCA. Formalin-Fixed Paraffin-Embedded (FFPE) tissues of 42 CCA patients were included in this study. PCR-based screening for HPyVs was conducted using degenerated and HPyV-specific primers. Following that, we performed FISH, RNA in situ hybridization (RNA-ISH), and immunohistochemistry (IHC) to assess the presence of HPyVs in selected tissues. Of all 42 CCAs, 25 (59%) were positive for one HPyV, while 10 (24%) CCAs were positive for 2 HPyVs simultaneously, and 7 (17%) were negative for HPyVs. Of the total 35 positive CCAs, 19 (45%) were positive for HPyV7, 4 (9%) for HPyV6, 2 (5%) for Merkel cell polyomavirus (MCPyV), 8 (19%) for both HPyV7/MCPyV, and 2 (5%) for both HPyV6/HPyV7 as confirmed by sequencing. The presence of viral nucleic acids was confirmed by specific FISH, while the RNA-ISH confirmed the presence of HPyV6 on the single-cell level. In addition, expression of HPyV7, HPyV6, and MCPyV proteins were confirmed by IHC. Our results strongly indicate that HPyV7, HPyV6, and MCPyV infect bile duct epithelium, hepatocytes, and CCA cells, which possibly suggest an indirect role of these viruses in the etiopathogenesis of CCA. Furthermore, the observed hepatotropism of these novel HPyV, in particular HPyV7, might implicate a role of these viruses in other hepatobiliary diseases.

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“…Given the correlation between latent TSPyV infections in immunocompromised patients, Kamminga et al were interested in the contribution of blood components as a possible vehicle for TSPyV transmission. In a large group of Dutch blood donors, TSPyV DNA was detected in five donors (0.5%) 38 . Infectivity of TSPyV in donor blood and the transmission via blood components to immunocompromised transplant recipients require more investigation 38 …”

Section: Epidemiologymentioning

confidence: 99%

“…In a large group of Dutch blood donors, TSPyV DNA was detected in five donors (0.5%) 38 . Infectivity of TSPyV in donor blood and the transmission via blood components to immunocompromised transplant recipients require more investigation 38 …”

Section: Epidemiologymentioning

confidence: 99%

Recent developments in trichodysplasia spinulosa disease

Narayanan

Rády

Tyring

2020

Transplant Infectious Dis

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Trichodysplasia Spinulosa (TS) is a rare proliferative skin disease that occurs primarily in immunocompromised patients, specifically organ transplant recipients. TS is characterized by uncontrolled inner root sheath cell proliferation and folliculocentric papular eruption that can progress to disfiguring leonine facies when left untreated. TS presents with distinct histological features including the presence of large eosinophilic, trichohyaline granules within hyperproliferating inner root sheath cells of the hair bulb. The discovery of the Trichodysplasia Spinulosa Polyomavirus (TSPyV) and recent studies highlighting the role of TSPyV tumor antigens in cell proliferation pathways have provided new insight into the mechanisms of TS development. In this review, we discuss the expansion of our understanding of TS, specifically over the past 5 years. We summarize novel cases of TS and recent developments in the mechanisms underlying TSPyV-mediated disease progression. We also evaluate advancements in diagnostic methods and treatment options. As the incidence of TS continues to rise, it is becoming critical for clinicians to understand the clinical features of TS and emerging research regarding pathogenesis and therapeutics for early treatment of this potentially disfiguring disease.

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Prevalence of DNA of fourteen human polyomaviruses determined in blood donors

Cited by 25 publications

References 56 publications

Prevalence and viral loads of polyomaviruses BKPyV, JCPyV, MCPyV, TSPyV and NJPyV and hepatitis viruses HBV, HCV and HEV in HIV-infected patients in China

Prevalence and viral loads of polyomaviruses BKPyV, JCPyV, MCPyV, TSPyV and NJPyV and hepatitis viruses HBV, HCV and HEV in HIV-infected patients in China

High Prevalence of Human Polyomavirus 7 in Cholangiocarcinomas and Adjacent Peritumoral Hepatocytes: Preliminary Findings

Recent developments in trichodysplasia spinulosa disease

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