Prevalence of Dog Erythrocyte Antigens 1, 4, and 7 in Podenco Ibicenco (Ibizan Hounds) from Ibiza Island

Spada, Eva; Proverbio, Daniela; Flórez, Luis Miguel Viñals; Serna, Blanca Serra Gómez de la; Chamizo, Maria del Rosario Perlado; Baggiani, Luciana; Perego, Roberta

doi:10.1155/2016/1048257

Cited by 10 publications

(24 citation statements)

References 18 publications

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“…The data collected from the website DbD has been organized as follows:The prevalence of DEA 1 negative and positive types in the total population and in breeds that showed a substantial number of subjects registered (over 50) was calculated.The breeds numbering over 50 subjects was subtyped as females and males and the prevalence of DEA 1 was calculated according to sex.In the breeds numbering over 50 subjects the percentage of the breed's population was determined in relation to the total Italian canine population inferred from the last 10 years of registration at the ENCI (Ente Nazionale di Cinofilia Italiana, Italian National Kennel Club) (http://www.enci.it) which was calculated.The data concerning the provenience and corresponding blood type of the 7,414 dogs was subdivided into different geographical regions of Italy.Using the standard inclusion criteria for canine blood donors (dogs heavier than 25 kg and aged between 2 and 8 years) and breeds numbering over 20 subjects were considered as prospective “blood donors” regardless of whether they were DEA 1 positive or negative.The risk of sensitization for each breed (over 50 subjects) was calculated using the formula [(% DEA 1 negative × % DEA 1 positive)/100] to establish the probability of a dog to become sensitized after the first transfusion of blood without having been tested with a cross-match and typed for DEA 1 [28, 30, 31]. …”

Section: Methodsmentioning

confidence: 99%

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Valentin

Gavazza

Lubas

2017

Veterinary Medicine International

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The study aim was to establish the prevalence of DEA 1, the most immunogenic and clinically important blood group in canine blood transfusion, in 7,414 dogs from Italy. The potential sensitization risk following a first transfusion and the acute reaction risk following a second transfusion given without a cross-matching and blood typing test were also calculated. Dogs tested were purebred (4,798) and mongrel (2,616); 38.8% were DEA 1 negative and 61.2% were DEA 1 positive. High prevalence for DEA 1 positive blood type was found in Ariegeois and English Setter, whereas German Shepherd and Boxer had higher DEA 1 negative blood type. Breeds with blood type never reported before included French Brittany Spaniel and Pug showing a high prevalence of DEA 1 positive type, while French Bulldog and West Highland White Terrier were more often DEA 1 negative. Just 48.8% of purebred and 13.9% of mongrel dogs were considered as prospective blood donors based upon their blood type. Most of the breeds had a sensitization risk of 20.0–25.0%. Rottweiler and Ariegeois had less risk of sensitization (9.4 and 4.2%) and the minor risk of an acute transfusional reaction (0.9–0.2%). The prevalence of DEA 1 positive and negative dogs in Italy agrees with most of the data already reported in the literature.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Valentin

Gavazza

Lubas

2017

Veterinary Medicine International

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“…Blood typing methods have evolved from tube and card tests to immunochromatographic strip, cartridge, and flow cytometry assays, but additional typing kits for other blood types would be desirable (1,(21)(22)(23)(24). Several limited surveys have been performed for DEA 1, and a few have assessed other blood types in North America and Europe (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35), but there have been no comprehensive surveys carried out on a canine population typing for many blood groups.…”

Section: Introductionmentioning

confidence: 99%

Survey of Blood Groups DEA 1, DEA 4, DEA 5, Dal, and Kai 1/Kai 2 in Different Canine Breeds From a Diagnostic Laboratory in Germany

et al. 2020

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More than twelve blood group systems have been described in dogs, but little is known about their distribution frequencies within breed populations. Here, we report on an extensive typing survey carried out using available reagents and either established or new clinical kits in purebred dogs from Germany. Leftover anticoagulated blood samples were examined using an immunochromatographic strip method for DEA 1, a gel column technique for Dal and Kai 1/2, and new card agglutination tests for DEA 4 and DEA 5 (which were partially compared with the gel column technique). Monoclonal antibodies were used for DEA 1 and Kai 1/2 typing, and polyclonal antibodies were used for all other types. Among the 206 dogs, 59.2% were DEA 1+, 100% DEA 4+, 9% (Card)/11% (Gel) DEA 5+, 89.3% Dal+, 96.6% Kai 1+, and 2.9% Kai 2+. None of the dogs were Kai 1+/2+, and only one was Kai 1-/2-. Dal-dogs were found in several breeds. Erythrocytes from most DEA 1+ dogs bound strongly on the strips. The agglutination reactions for DEA 5 on the new card tests were generally less than those on the gel column. The blood group pattern DEA 4+, DEA 5-, Dal+, Kai 1+/2-and either DEA 1+ or DEA 1-was found among 80% of the dogs. In this first extensive blood typing survey of purebred dogs from Europe, the proportions of positive and negative blood types were similar to those found in the United States and, for DEA 1, were also similar to those from other European countries, with considerable breed variation in blood types. The newer typing techniques seem to work well and will likely be useful for detecting and preventing specific blood type incompatibilities in the clinic.

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“…DEA 1 is the most studied blood type. The prevalence of DEA 1-positive dogs varies both geographically and among breeds from 100% to <10%, but has been estimated at ~50% overall internationally (Giger et al, 1995;Novais et al, 1999;Hale et al, 2008;Iazbik et al, 2010;Sinnott Esteve et al, 2011;Ergul Ekiz et al, 2011;Spada et al, 2015aSpada et al, , 2015bSpada et al, , 2016cEuler et al, 2016). DEA 1 is the most antigenic blood type for which naturally occurring antibodies do not exist (Giger et al, 1995;Hale and Wefelmann, 2006;Blais et al, 2009;Euler et al, 2016) .…”

Section: Introductionmentioning

confidence: 99%

Dog erythrocyte antigens (DEA) 1, 4, 7 and suspected naturally occurring anti-DEA 7 antibodies in Italian Corso dogs

Spada

Proverbio

Priolo

et al. 2017

The Veterinary Journal

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We sought to determine the prevalence of dog erythrocyte antigen (DEA) 1, 4 and 7 and naturally occurring anti-DEA7 antibodies in Italian Corso dogs. In addition, we correlated DEAs with different epidemiologic variables, compared the prevalence of DEAs against other canine populations and assessed the risk of sensitisation and transfusion reactions (TRs) following unmatched transfusion. Blood samples from 100 Corso dogs were evaluated for DEA 1, 4, 7 and naturally occurring anti-DEA 7 antibodies. Seventy-one percent of samples were DEA 1-negative, 100% tested DEA 4-positive, and 95% tested DEA 7-negative. Suspected anti-DEA7 antibodies were found in 32% dogs. The DEA 1 and 7-negative phenotypes were significantly more common than in most canine populations. When a previously tested Italian canine population was considered as blood donors for Corso dogs, the risk of DEA 1 sensitisation using DEA 1 untyped blood was 29%, and of acute haemolytic TRs after a second untyped DEA 1-incompatible transfusion was 8%. The potential for delayed TRs between DEA 7-negative Corso dogs with suspected naturally occurring anti-DEA 7 antibodies receiving untyped DEA 7-positive blood was 11%. Conversely, when Corso dogs were blood donors for the same population, the risk of DEA 1 sensitisation was 17% and the risk of an acute haemolytic TR after a second DEA 1-incompatible blood transfusion was 3%. Corso dogs can be suitable blood donors. Additional studies are needed to clarify whether the high prevalence of naturally occurring anti-DEA 7 antibodies in this breed could increase their risk of delayed TRs when they are blood recipients.

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Prevalence of Dog Erythrocyte Antigens 1, 4, and 7 in Podenco Ibicenco (Ibizan Hounds) from Ibiza Island

Cited by 10 publications

References 18 publications

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy

Survey of Blood Groups DEA 1, DEA 4, DEA 5, Dal, and Kai 1/Kai 2 in Different Canine Breeds From a Diagnostic Laboratory in Germany

Dog erythrocyte antigens (DEA) 1, 4, 7 and suspected naturally occurring anti-DEA 7 antibodies in Italian Corso dogs

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