Prevalence of E. coli O157:H7 in water sources: an overview on associated diseases, outbreaks and detection methods

Saxena, Tanushree; Kaushik, Pallavi; Mohan, M. Krishna

doi:10.1016/j.diagmicrobio.2015.03.015

Cited by 118 publications

(75 citation statements)

References 263 publications

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“…Indeed, in 2005, in northwestern France where our study sites were located, EHEC O26:H11 were involved in a human outbreak due to raw milk cheese (INVS, 2007). Although STEC have been reported to be prevalent in wild animals living close to a cattle farm (Saxena et al, 2015), it is likely that these pathogenic E. coli came from the cattle reservoir. Indeed, in our study, no pathogenic strain was isolated in the site close to the forest (Sébec) while high abundance of unassignable B2 strains suggested the presence of E. coli from wild animals, mainly boar, roe and deer (Smati et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The contamination of water by Shiga toxin-producing E. coli (STEC/EHEC), mainly E. coli O157:H7 but also non-O157 STEC, have resulted in numerous outbreaks associated with both recreational and drinking water (Saxena et al, 2015). In France, the main STEC associated with severe gastroenteritis, related to food or water consumption, belong to the five highly pathogenic serotypes O157:H7; O26:H11, O111:H8, O103:H2, and O145:H28 and more recently O80:H2 (INVS, 2007; ANSES, 2010; Delannoy et al, 2015; Soysal et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Three main outbreaks associated with STEC/EHEC have been there reported (O26:H11, O80:H2, and O157:H - ), due to the consumption of cheese made with raw milk (INVS, 2007; ANSES, 2010). The main reservoir of STEC/EHEC being the digestive tract of cattle, the runoff on pastured lands is associated to the contamination of environmental water by these highly pathogenic E. coli (Chandran and Mazumder, 2015; Saxena et al, 2015). Thus, water contaminated by STEC/EHEC is a pathway for the dissemination of these highly pathogenic E. coli between the three main reservoirs: animals, water, and humans.…”

Section: Introductionmentioning

confidence: 99%

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Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Petit

Clermont

Delannoy³

et al. 2017

Front. Microbiol.

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The aim of this study was to investigate the diversity of the Escherichia coli population, focusing on the occurrence of pathogenic E. coli, in surface water draining a rural catchment. Two sampling campaigns were carried out in similar hydrological conditions (wet period, low flow) along a river continuum, characterized by two opposite density gradients of animals (cattle and wild animals) and human populations. While the abundance of E. coli slightly increased along the river continuum, the abundance of both human and ruminant-associated Bacteroidales markers, as well as the number of E. coli multi-resistant to antibiotics, evidenced a fecal contamination originating from animals at upstream rural sites, and from humans at downstream urban sites. A strong spatial modification of the structure of the E. coli population was observed. At the upstream site close to a forest, a higher abundance of the B2 phylogroup and Escherichia clade strains were observed. At the pasture upstream site, a greater proportion of both E and B1 phylogroups was detected, therefore suggesting a fecal contamination of mainly bovine origin. Conversely, in downstream urban sites, A, D, and F phylogroups were more abundant. To assess the occurrence of intestinal pathogenic strains, virulence factors [afaD, stx1, stx2, eltB (LT), estA (ST), ipaH, bfpA, eae, aaiC and aatA] were screened among 651 E. coli isolates. Intestinal pathogenic strains STEC O174:H21 (stx2) and EHEC O26:H11 (eae, stx1) were isolated in water and sediments close to the pasture site. In contrast, in the downstream urban site aEPEC/EAEC and DAEC of human origin, as well as extra-intestinal pathogenic E. coli belonging to clonal group A of D phylogroup, were sampled. Even if the estimated input of STEC (Shiga toxin-producing E. coli) – released in water at the upstream pasture site – at the downstream site was low, we show that STEC could persist in sediment. These results show that, the run-off of small cattle farms contributed, as much as the wastewater effluent, in the dissemination of pathogenic E. coli in both water and sediments, even if the microbiological quality of the water was good or to average quality according to the French water index.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Petit

Clermont

Delannoy³

et al. 2017

Front. Microbiol.

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“…Waterborne diseases have a significant impact on public health and are associated with a high morbidity and mortality rate in developing countries (Saxena et al ). STEC was identified in drinking water for human consumption in PR and RS (Sandrini et al .…”

Section: Occurrence Of Stec In Foodmentioning

confidence: 99%

Shiga toxin‐producingEscherichia coliin the animal reservoir and food in Brazil

Gonzalez

Cerqueira

2019

J Appl Microbiol

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Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC contaminating the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7 remains the most frequent serotype associated with disease. In Brazil, the prevalence of STEC reaches values as high as 90% in cattle and 20% in meat products which may impact the Brazilian food export trade. However, only few reports are related to human disease. The stx1 gene prevails in cattle, whereas the stx2 gene is more frequent in food. Several STEC serotypes have been isolated from cattle and food in Brazil, including the O157:H7, O111:NT, NT:H19 as well as O26 and O103 serogroups. O113: H21 STEC strains are frequent in ruminants and foods but with no report in human disease. The virulence profile of Brazilian STEC strains from cattle and food suggests a pathogenic potential to humans, although some differences with clinical strains have been detected. Further studies, employing recent and more discriminative techniques are in need to better clarify their virulence potential.

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“…Pathogenic Escherichia coli (E. coli) is one of the most harmful bacteria related to food-borne diseases and can cause inflammation, abdominal pain, diarrhea and even severe cases like hemorrhagic enteritis and hemolysis, especially in infants and youngsters [1]. A systematic analysis indicated that E. coli infection has already become the second leading cause of death in children younger than 5 years old [2].…”

Section: Introductionmentioning

confidence: 99%

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

et al. 2019

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An aptamer-based electrochemical biosensor was successfully developed and applied in the rapid detection of pathogenic Escherichia coli (E. coli) in licorice extract. The thiolated capture probes were firstly immobilized on a gold electrode, and then the biotinylated aptamer probes for E. coli were introduced by hybridization with the capture probes. Due to the stronger interaction between the aptamer and the E. coli, a part of the biotinylated aptamers will dissociate from the capture probes in the presence of E. coli. The residual biotinylated aptamer probes can quantitatively bind with streptavidin-alkaline phosphatase. Subsequently, α-naphthyl phosphate substrate was catalytically hydrolyzed to generate electrochemical response, which could be recorded by a differential pulse voltammetry. The dependence of the peak current on the logarithm of E. coli concentration in the range from 5.0 × 10 2 colony forming units (CFU)/mL to 5.0 × 10 7 CFU/mL exhibited a linear trend with a detection limit of 80 CFU/mL. The relative standard deviation of 5 successive scans was 5.3%, 4.5% and 1.1% for 5.0 × 10 2 , 5.0 × 10 5 and 5.0 × 10 7 CFU/mL E. coli, respectively. In the detection of the licorice extract samples, the results obtained from the proposed strategy and traditional culture counting method were close to each other, but the time consumption was only~1/30 compared with the traditional method. These results demonstrate that the designed biosensor can be potentially utilized for rapid microbial examination in traditional Chinese medicine and relevant fields. Appl. Sci. 2019, 9, 295 2 of 15 bacterial colony morphology, color change, and biochemical reactions for quantitative detection. This detection method is economical and relatively simple to operate, but the detection time is generally 48-72 h or even longer, which cannot meet the needs for rapid detection. As an effective molecular biological detection technology, polymerase chain reaction (PCR) and the derived technology of PCR has been widely used in detecting E. coli [4,7]. PCR is based on the principle that DNA templates can denature at high temperatures, reactivate and extend (one cycle) at low temperatures by using appropriate DNA polymerase and primers. As the template DNA amplification product increases exponentially, increasing the number of cycles can magnify the specified DNA sequence by several million-fold in a short time, which greatly improves detection sensitivity [8]. However, PCR technology is costly and prone to produce false negatives [9], which limits the extensive application of PCR. In addition, enzyme-linked immunosorbent assay (ELISA), as a common immunological detection technology, has also been used in bacterial test [5,10]. ELISA technology undergoes a color change by the catalysis of the enzyme in the specific recognition process. Owing to the immune activity between antigen and antibody, ELISA method has superior specificity, high sensitivity and short detection time, and has been used in detection of different subtypes of E. col...

show abstract

Prevalence of E. coli O157:H7 in water sources: an overview on associated diseases, outbreaks and detection methods

Cited by 118 publications

References 263 publications

Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Shiga toxin‐producingEscherichia coliin the animal reservoir and food in Brazil

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

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