Prevalence of Enterotoxin Genes in<i>Staphylococcus aureus</i>Isolates from Pork Production

Zhang, Yang; Wang, Yage; Cai, Rujian; Shi, Lei; Li, Chunling; He, Yan

doi:10.1089/fpd.2017.2408

Cited by 21 publications

(12 citation statements)

References 36 publications

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“…The most prevalent toxin genes were the egc encoded genes seg , sei , sem , sen , seo and seu , which were detected in 11 strains belonging to CC1, CC7, CC9, CC49, and CC398. On conventional cuts of pork meat similar enterotoxin profiles have been reported [10,45]. Interestingly, only one strain isolated from a heart harbored the sec and sel genes.…”

Section: Resultssupporting

confidence: 67%

Microarray based genetic profiling of Staphylococcus aureus isolated from abattoir byproducts of pork origin

et al. 2019

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Many parts of pork meat processing are currently not used for human consumption in Switzerland, although they are of great nutritional value. Therefore, data on the occurrence of pathogenic organisms on byproducts is extremely scarce and the prevalence and population structure of Staphylococcus aureus on meat processing sidestreams is unknown. Hence, abattoir byproducts of pork origin including ear, forefoot, heart, intestine, liver, rib bone, sternum, bladder, stomach, hind foot and tongue originating from six abattoirs were screened for S . aureus . The obtained isolates were investigated by spa typing and DNA microarray analysis to reveal their genomic profile and population structure. The prevalence of S . aureus was generally low with a mean of 8%. In total, 40 S . aureus strains were detected and assigned to 12 spa types (t015, t1491, t1778, t091, t337, t899, t2922, t7439, t1333, t208, t4049, t034) and seven clonal complexes (CC1, CC7, CC9, CC30, CC45, CC49, CC398). Detected enterotoxin genes included sea , seb , sec , seh , sel and egc encoded toxin genes seg , sei , sem , sen , seo , and seu . None of the isolates harbored genes conferring methicillin resistance, but blaZ/I/R genes causing penicillin resistance were frequently found. In addition, strains from CC398 exhibited tetM and tetK , conferring tetracycline resistance. Similarity calculations based on microarray profiles revealed no association of clonal complexes with particular body parts, but revealed a certain correspondence of clonal complex and originating abattoir.

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Section: Resultssupporting

confidence: 67%

Microarray based genetic profiling of Staphylococcus aureus isolated from abattoir byproducts of pork origin

et al. 2019

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“…According to our phenotypic tests, a S. aureus isolate, which also harboured the seb gene, was positive for enterotoxin B production (Table 4). In parallel to the present study, the prevalence of se genes of S. aureus isolates from pork were also high at rates of 94Á6 and 75% in China (Zhang et al 2018) and Poland (Bystron et al 2018) respectively. According to our phenotypic tests, a S. aureus isolate, which also harboured the seb gene, was positive for enterotoxin B production (Table 4).…”

Section: Prevalence Of Se and Tst Genessupporting

confidence: 78%

Occurrence, antibiotic resistance and enteroxigenicity of Staphylococcus spp. in tonsils of slaughtered pigs in Greece

Pexara

Σολωμάκος

Govaris

2020

Lett Appl Microbiol

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The aims of the present study were to examine the occurrence of Staphylococcus spp. in the tonsils of slaughtered pigs in a regional slaughterhouse in Greece, the antibiotic resistance of the Staphylococcus spp. isolates, and the enteroxigenicity of the S. aureus isolates. Staphylococcus spp. were isolated in 70 (48·61%) out of the total 144 tonsil samples. The predominant species was S. aureus in coagulase‐positive staphylococci (CoPS), while the predominant species were Staphylococcus epidermidis and Staphylococcus saprophyticus in the coagulase‐negative staphylococci (CoNS). Staphylococcus spp. isolates presented high antibiotic resistance frequencies to tetracycline (97·1%) or clindamycin (80·0%) and low antibiotic resistance frequencies to fusidic acid (14·3%). No methicillin‐resistant S. aureus (MRSA) strains were identified, and all Staphylococcus spp. isolates were susceptible to vancomycin. Among the 26 S. aureus isolates, 21 (80·76%) possessed staphylococcal enterotoxin genes with seven different enterotoxin gene profiles. The predominant enterotoxin profile was seg, sei and sej with seven S. aureus isolates. The occurrence of multidrug resistant Staphylococcus spp. in pig tonsils indicate public health risk to pork consumers and handlers in developing antimicrobial resistance.

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“…In this study, 68 out of 385 raw goat milk samples (17.6%, 68/385) were positive with S. aureus . The prevalence of S. aureus is significantly lower than that in the previous report that showed the contamination rate of S. aureus in pork industry was 26% (130/501) in China [28]. Similarly, it was also obviously less than that in other reports which demonstrated that S. aureus was detected in 53.5% (153/286) of the bulk tank milk samples in Italy [29], and 46% (47/104) of the bulk tank milk samples in the United States [30].…”

Section: Discussionmentioning

confidence: 65%

Epidemiological Characteristics of Staphylococcus Aureus in Raw Goat Milk in Shaanxi Province, China

Qian

Shen

et al. 2019

Antibiotics

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Goat milk has been frequently implicated in staphylococcal food poisoning. The potential risk of raw goat milk contaminated by Staphylococcus aureus (S. aureus) in Shaanxi province of China is still not well documented. This study investigated the prevalence, antibiotic resistance, as well as virulence-related genes of S. aureus from raw goat milk samples in Shaanxi, China. A total of 68 S. aureus isolates were cultured from 289 raw goat milk. Most of the isolates were resistant to penicillin and oxacillin, although 41.18%, 33.82%, and 29.41% of the isolates expressed resistance to piperacillin, trimethoprim-sulfamethoxazole, and ciprofloxacin, respectively. Our data demonstrated that 91.18% of the isolates produced biofilm, of which 54.41% isolates belonged to high-biofilm producers. In addition, genotypic analysis of biofilm related genes (fnbA, clfB, fnbB, cna) revealed that 91.18% of the isolates harbored at least one of the genes, in which the most prevalent genes were fnbA (66. 17%), clfB (48.53%), and fnbB (26.47%). 94.8% of the isolates contained at least one toxin-related gene, of which seb (76.47%), tsst (36.76%), and sea (23.53%) genes were the more frequently detected. Further analysis revealed a positive association between fnbA, clfB, fnbB, seb, tsst, and sea genes and certain antibiotic resistance. The results indicated that raw goat milk samples contaminated by S. aureus can be a potential risk to public health.

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Prevalence of Enterotoxin Genes inStaphylococcus aureusIsolates from Pork Production

Cited by 21 publications

References 36 publications

Microarray based genetic profiling of Staphylococcus aureus isolated from abattoir byproducts of pork origin

Microarray based genetic profiling of Staphylococcus aureus isolated from abattoir byproducts of pork origin

Occurrence, antibiotic resistance and enteroxigenicity of Staphylococcus spp. in tonsils of slaughtered pigs in Greece

Epidemiological Characteristics of Staphylococcus Aureus in Raw Goat Milk in Shaanxi Province, China

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