Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali

Abstract

Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients' blood cultures in two university teaching hospitals of Bamako, Mali. Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results: The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae. Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively). Conclusion: Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.

Multi-drug resistant, extended spectrum beta-lactamase and carbapenemase-producing bacterial isolates among children under five years old suspected bloodstream infection in a specialized Hospital in Ethiopia: Cross-sectional study

“…Among 43 Klebsiella pneumoniae isolates 14(32.5%) and 5 E.coli isolates 1(20.0%) are ESBL-producers. This is in keeping with the study conducted in south India by Zakariya et al, 32.0% [54] and in Mali by Sangare et al, 29.4% [57] for ESBL producing Klebsiella pneumoniae.…”

Multi-Drug Resistant, Extended Spectrum β-Lactamase and Carbapenemase Producing Bacterial Isolates among Septicemia Suspected under Five Children in Tikur Anbesa Specialized Hospital, Addis Ababa Ethiopia

“…The prevalence of ESBL-producing Enterobacteriaceae in our study is 25.4%. Among 43 Klebsiella pneumoniae isolates 14(32.5%) and 5 E.coli isolates 1(20.0%) ESBL-producers which is in line with the study conducted in south India by Zakariya et al, 32.0% [ 57] and in Mali by Sangare et al, 29.4% [60] ESBL producing Klebsiella pneumoniae.…”

Microbial Profile of Blood Stream Infections and their Antibiotic Susceptibility Pattern of Isolates among Paediatric Patients admitted in a Teaching Hospital of West Bengal

“…coli were 100% ESBL producers, while they found 41.7%. [23] In this study Pseudomonas aeruginosa were susceptible to Amikacin 55.6 %, Aztreonam and Piperacillin-tazobactam66.7% and Piperacillin, Ceftazidime and Ciprofloxacin 44.4%, Gentamicin 33.3% which can be compared by Mohammad A et al, Amikacin 100%, Aztreonam 33.3%, Ceftazidime 33.3%, Ciprofloxacin 100%, Gentamicin 66.6 % and Piperacillin 66.6%. [19] Among Acinetobacter spp.…”