Prevalence of Escherichia Coli from Pigs and Cattle

Abstract

Escherichia coli a gram negative rod shaped bacteria has been shown to be normal inhabitant of the gastrointestinal tract of animals and man and most of them are harmless, but some of the serotypes of it are pathogenic and causes diarrhoea and extra intra-intestinal infections in human and animals. A study was done to find out the prevalence of Escherichia coli from pigs and cattle from organized and unorganized farm in Assam. A total of 260 faecal samples, comprising 110 from cattle and 150 from pigs were examined for the presence of E.coli. The overall prevalence of E.coli in cattle was recorded as 67.27%. The prevalence was recorded as 76.67% and 56.00% in diarrhoeic cattle and non-diarrhoeic cattle, respectively. The overall prevalence of E.coli in pigs was recorded as 59.33%. The prevalence was recorded as 70.59% and 44.62% from diarrhoeic pigs and non-diarrhoeic pigs, respectively.All copyrights reserved to Nexus® academic publishers The samples were collected aseptically in sterile vials from organized and unorganized farm of Assam. A total of 260 faecal samples, comprising 110 from cattle and 150 from pigs were examined for the presence of E.coli. All the samples were collected aseptically in sterile vials, immediately brought to the laboratory for bacteriological culture. The commercially available readymade Mac Conkey broth and Mac Conkey lactose agar media (HiMedia, Mumbai) was used for bacterial isolation, identification and characterization of E. coli. Faecal samples were first inoculated in Mac Conkey's broth and incubated at 37°C overnight. For primary isolation of E.coli Mac Conkey lactose agar is used, samples were inoculated on Mac Conkey lactose agar plates and incubated at 37°C overnight. Morphological characteristics like shape, size, arrangement and staining reaction of the organisms of each isolates were studied after staining the fresh culture smears with Gram's stain.The prevalence of E.coli in diarrhoeic and non diarrhoeic adult cattle as well as calves was recorded. A total of 110 faecal samples were collected out of which 74 (67.27%) samples were found positive to E.coli. The prevalence was found to be 60% and 88.57% in diarrhoeic adult cattle and calves, respectively whereas it was found to be 53.33%and 60% in non diarrhoeic cattle and calves, respectively. The overall prevalence of E.coli in diarrhoeic cattle and non-diarrhoeic cattle was found to be 76.67% and 56.00%, respectively. Trend on the prevalence of E. coli in both diarrhoeic and non diarrhoeic adult cattle and calves is shown graphically in (Figure 1).A total of 150 faecal samples from pigs were examined out of which 89 were found to be positive for E.coli.

Prevalence of Subclinical Mastitis in Dairy Goats Caused by Bacterial Species

“…The higher incidence of Staphylococcus aureus in milk samples of subclinical mastitis of goats was recorded in this survey was also demonstrated by above workers. Escherichia coli are known as the most common environmental contaminants because these are found as a normal flora in the intestine of almost all farm animals (Ansari et al, 2014;Begum et al, 2014). From above workers, Sarker and Samad (2011) reported the incidence of Escherichia coli as a second most in subclinical mastitis milk samples, however in the present study we observed the Bacillus subtilis as second most prevalent organism.…”

Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves

“…Our results showed that 95% of the collected samples were positive E. coli. the result is agreed with the finding previously obtained by (Begum et al, 2014), where 88.5 % of fecal samples were positive E. coli. While a lower percentage was obtained by (Oporto et al, 2008)), where only 35.9% of the examined samples were positive for E. coli.…”

Epidemiology and antibiogram of common mastitis-causing bacteria in Beetal goats

“…As E. coli is mostly found on farm animals, it has been recognized as the most common environmental contaminant [ 59 , 60 ]. Our findings are very close to that of Moroni et al .…”