Prevalence of Escherichia coli O157 isolated from marketed raw cow milk in Kwara State, Nigeria

Ghali-Mohammed, Ibraheem; Odetokun, Ismail A.; Raufu, Ibrahim A.; Alhaji, Nma Bida; Adetunji, Victoria O.

doi:10.1016/j.sciaf.2022.e01469

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…The detection of E. coli O157 in milk and dairy products as well as its antimicrobial resistance have been reported in several studies from around the world as it is a growing concern [58][59][60][61][62][63] . Depending on the level of hygiene standards and milking and handling procedures, and possibly the detection method followed, the incidence may differ between nations and among dairy farms.…”

Section: Antimicrobial Susceptibility Profile Of E Coli and E Coli O157mentioning

confidence: 99%

Occurrence and antimicrobial susceptibility patterns of Escherichia coli and Escherichia coli O157 isolated from cow milk and milk products, Ethiopia

Sarba,

Wirtu,

Gebremedhin

et al. 2023

Sci Rep

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Escherichia coli is a major foodborne pathogen worldwide. This study was conducted to assess the prevalence, risk factors, and antimicrobial susceptibility of E. coli and E. coli O157 in milk and milk products and hygienic practices in West Shoa, Oromia, Ethiopia. Five hundred fifty-six milk samples comprising 421 udder milk, 57 bulk tank milk, and 78 milk products were investigated. Moreover, a questionnaire was administered to 145 participants to assess hygienic practices. A standard microbiological procedure was used to detect E. coli and E. coli O157. The Kirby Bauer disc diffusion method was used to test the antimicrobial susceptibility of the isolates. Petri film plates were used to enumerate the coliform in raw bulk tank milk. Farm and animal-level E coli prevalence were estimated and association with risk factors was assessed. Escherichia coli was detected in 33.8% (95% confidence interval (CI) 29.9–37.9%) of the samples, of which only one isolate (0.2%) was E. coli O157. Escherichia coli contamination was higher in bulk tank samples (47.4%; 95% CI 34.0–61.0%) than in udder milk (34.7%; CI 30.1–39.4%), cottage cheese (27.0%; 95% CI 14.6–43.9%), and yoghurt 10.5% (95% CI 2.9–24.8%). For the animal-level E. coli contamination, only the study area was identified as a risk factor. Risk factors such as types of milk containers, udder washing practices, hygiene, and management systems were associated with both farm-level and milk products E. coli contamination. Most (59.0%) of the farmers practice udder washing only before milking and the remaining did not practice udder washing at all. The mean coliform count in raw bulk tank milk was higher than the international standard (4.09 log10 CFU/ml). All the 42 E. coli isolates tested were multidrug resistant. The occurrence of E. coli is high, while that of E. coli O157 is too low. The milk-handling practices are poor in the study areas. The high prevalence of E. coli, as well as the high coliform count and higher multi-drug resistance may pose risk to public health and food safety. Therefore, proper hygienic practices throughout the milk chain as well as rational drug use are advised.

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Section: Antimicrobial Susceptibility Profile Of E Coli and E Coli O157mentioning

confidence: 99%

Occurrence and antimicrobial susceptibility patterns of Escherichia coli and Escherichia coli O157 isolated from cow milk and milk products, Ethiopia

Sarba,

Wirtu,

Gebremedhin

et al. 2023

Sci Rep

View full text Add to dashboard Cite

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“…Previous studies have commonly shown unsatisfactory counts of microorganisms and/or microbial groups used to indicate the food hygienic-sanitary conditions in street food samples and the possible risks for the occurrence of foodborne disease outbreaks. These studies also indicate that food contamination should be reduced by avoiding common hazards in food production and monitoring the maintenance of GMP [37,51,52]. Studies with foods commercialized by family farmers have also reported unsatisfactory microbiological characteristics linked to not adopting GMP [12,13].…”

Section: Discussionmentioning

confidence: 94%

“…Cheeses are among the foods most involved in foodborne outbreaks, being incriminated in about 4% of all foodborne illnesses, which are more frequent in developing countries [51]. Coliforms, coagulase-positive staphylococci, and molds and yeasts are frequently found and considered indicators of food contamination by improper handling and a higher risk of foodborne disease [51,52].…”

Section: Discussionmentioning

confidence: 99%

Development of an Educational Gamification Strategy to Enhance the Food Safety Practices of Family Farmers in Public Food Markets of Northeast Brazil: A Case Study

Rodrigues

Bezerra

Pereira

et al. 2023

Foods

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This study aimed to develop an educational gamification strategy to enhance the food safety practices of family farmers in public food markets in a city in Northeastern Brazil (João Pessoa, PB, Brazil). A good manufacturing practices (GMP) checklist was used to verify hygienic-sanitary conditions in the food markets. Educational game tools addressing foodborne diseases and GMP with information about the prevention of foodborne diseases, good food handling practices, and safe food storage were developed. Pre- and post-training assessments were done to evaluate food handlers’ knowledge and food safety practices. Microbiological parameters of food samples were analyzed before and two months after the training. Results indicated unsatisfactory hygiene conditions in the examined food markets. There was a very strong positive correlation between “implementation of GMP” and “production and process controls” (R = 0.95; p ≤ 0.05) and between “production and process controls” and “hygiene habits of handlers” (R = 0.92; p ≤0.05). There was no homogeneity between answers before and after the training for the knowledge of family farmers regarding “prevention of foodborne diseases” and “safe food handling”. There were improvements in the measured microbiological parameters of foods sold by family farmers after the application of the developed educational gamification training. These results showed the developed educational game-based strategy as being effective in raising awareness of hygienic sanitary practices, helping to promote food safety, and reducing risks for the consumers of street foods at family farmers’ markets.

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“…Examples of documented cases in Africa are as follows: human infection that was reported in Johannesburg, South Africa in 1990 [70]; the detection of the pathogen in individuals with haemorrhagic colitis that resulted to fatalities in Bangui, Central African Republic [71]; and the isolation of the pathogen in East African countries (Tanzania, Kenya, and Ethiopia) [72]. The prevalence rates of STEC O157:H7 reported in Africa were 7% in Morogoro, Tanzania, amongst patients suffering from diarrhoea [72], 2.3% isolated from raw cattle milk in Kwara, Nigeria [73], 1.9% amongst children with diarrhoea in Mozambique [74], and 8% in HIV infected individuals suffering from dysentery in Zimbabwe [75].…”

Section: Epidemiology Of E Coli O157:h7mentioning

confidence: 99%

Safety Properties of Escherichia coli O157:H7 Specific Bacteriophages: Recent Advances for Food Safety

Oluwarinde,

Ajose,

Abolarinwa

et al. 2023

Foods

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Shiga-toxin-producing Escherichia coli (STEC) is typically detected on food products mainly due to cross-contamination with faecal matter. The serotype O157:H7 has been of major public health concern due to the severity of illness caused, prevalence, and management. In the food chain, the main methods of controlling contamination by foodborne pathogens often involve the application of antimicrobial agents, which are now becoming less efficient. There is a growing need for the development of new approaches to combat these pathogens, especially those that harbour antimicrobial resistant and virulent determinants. Strategies to also limit their presence on food contact surfaces and food matrices are needed to prevent their transmission. Recent studies have revealed that bacteriophages are useful non-antibiotic options for biocontrol of E. coli O157:H7 in both animals and humans. Phage biocontrol can significantly reduce E. coli O157:H7, thereby improving food safety. However, before being certified as potential biocontrol agents, the safety of the phage candidates must be resolved to satisfy regulatory standards, particularly regarding phage resistance, antigenic properties, and toxigenic properties. In this review, we provide a general description of the main virulence elements of E. coli O157:H7 and present detailed reports that support the proposals that phages infecting E. coli O157:H7 are potential biocontrol agents. This paper also outlines the mechanism of E. coli O157:H7 resistance to phages and the safety concerns associated with the use of phages as a biocontrol.

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Prevalence of Escherichia coli O157 isolated from marketed raw cow milk in Kwara State, Nigeria

Cited by 6 publications

References 35 publications

Occurrence and antimicrobial susceptibility patterns of Escherichia coli and Escherichia coli O157 isolated from cow milk and milk products, Ethiopia

Occurrence and antimicrobial susceptibility patterns of Escherichia coli and Escherichia coli O157 isolated from cow milk and milk products, Ethiopia

Development of an Educational Gamification Strategy to Enhance the Food Safety Practices of Family Farmers in Public Food Markets of Northeast Brazil: A Case Study

Safety Properties of Escherichia coli O157:H7 Specific Bacteriophages: Recent Advances for Food Safety

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