Prevalence of extended-spectrum β-lactamase (ESBL)-producing Salmonella enterica from retail fishes in Egypt: A major threat to public health

Gawish, Mahmoud F.; Ahmed, Ashraf M.; Torky, Helmy A.; Shimamoto, Tadashi

doi:10.1016/j.ijfoodmicro.2021.109268

Cited by 16 publications

(26 citation statements)

References 40 publications

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“…MDR was found in 25% (7/28) of food isolates and 14.29% (1/7) of human isolates, respectively. Previous characterizations of Salmonella isolates obtained from broiler meat and human, as well as from retail fish samples in other Egyptian provinces found that 68.1% and 100%, respectively, of isolates were either resistant to at least one antibiotic or carried MDR (Ahmed et al., 2014; Gawish et al., 2021; Hassan et al., 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Salmonella was detected in 4.83% (7/145) of human samples, which falls between the relatively low (4%) and high (9.3 and 12.6%) percentages reported in previous studies conducted in the Egyptian provinces of Beni-Suef (Hassan et al, 2016), Menoufia and Cairo (Abdelmalek et al, 2019) was found in 25% (7/28) of food isolates and 14.29% (1/7) of human isolates, respectively. Previous characterizations of Salmonella isolates obtained from broiler meat and human, as well as from retail fish samples in other Egyptian provinces found that 68.1% and 100%, respectively, of isolates were either resistant to at least one antibiotic or carried MDR Gawish et al, 2021;Hassan et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and molecular characterization of foodborne and human‐derived Salmonella strains for resistance to critically important antibiotics

Sabeq

Awad

Hamad

et al. 2022

Transbounding Emerging Dis

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The primary goals of this cross-sectional study were to screen various food/water, and human samples for the presence of Salmonella species, and to assess the phenotypic and genetic relationship between resistances found in food and human Salmonella isolates to critically important antibiotics. Between November 2019 and May 2021, 501 samples were randomly collected for Salmonella isolation and identification using standard culturing methods, biochemical, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and PCR techniques. Antimicrobial susceptibility testing was performed on confirmed Salmonella species, and PCR was used to investigate the genetic components that confer these resistance traits. Salmonella enterica subspecies enterica was confirmed in 35 (6.99%) of the samples (raw food = 23, ready-to-eat food/drink [REF/D] = 5, human = 7). Seventeen of them were antibioticresistant to at least one class, and eight were multidrug-resistant (MDR) isolates (raw food = 7, human = 1). All Salmonella isolates were susceptible to carbapenems, thirdand fourth-generation cephalosporins and monobactam antibiotics. Resistance phenotypes to aminoglycosides (48.57%), β-lactams (20%) and tetracycline (17.14%), as well as associated genes such as aadA, bla TEM , bla Z and tetA, as well as dfrA and sul1, were prevalent in Salmonella isolates. Colistin resistance genotype (mcr1) was detected in three (8.57%) isolates recovered from egg, cattle mince and rabbit meat, and the total incidence was 14.29% when two isolates exhibited resistance phenotypes were considered. Furthermore, four (11.43%) MDR isolates shared the bla TEM and bla Z genes, and one (2.86%) isolate contained three extended spectrum β-lactams producing genes (ESBL), namely bla CTX , bla TEM and bla Z . The gyrA gene was expressed by one of three foodborne Salmonella isolates (8.57%) with ciprofloxacin resistance phenotypes. To the best of our knowledge, this is the first report from Egypt identifying colistin resistance in Salmonella enterica recovered from cattle minced meat and rabbit meat. Overall, the highest incidence rate of Salmonella enterica was found in cattle-derived products, and it was slightly more prevalent in RTE/D foods than in raw foods. Resistance to critical and clinically important antibiotics, particularly in Salmonella from RTE/D food,

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization of foodborne and human‐derived Salmonella strains for resistance to critically important antibiotics

Sabeq

Awad

Hamad

et al. 2022

Transbounding Emerging Dis

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“…The presence and emergence of ESBL genes may be due to the exchange of plasmids IncA, IncY and IncX3 carrying these factors between gram-negative bacteria 7 . Furthermore, the occurrence of ESBL-harboring S. Typhi isolates has been reported from food and animal origin 13,14,24 .…”

Section: Discussionmentioning

confidence: 99%

“…Until the first decade of the 21 st century, ESBL-producing S. Typhi strains were uncommon, occurring only occasionally in some Asian countries 10,11 . However, in recent years, the emergence of MDR ESBL-producing Salmonella species, both from human and animal sources, has raised concerns [12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Extended-spectrum beta-lactamases encoding genes among <i>salmonella enterica</i> serovar typhi Isolates in patients with typhoid fever from four Academic Medical Centers in Lagos, Nigeria

Akinyemi¹,

Al-Khafaji²,

Al-Alaq³

et al. 2022

RIC

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Background: There is scarce information about the occurrence of extended-spectrum β-lactamases (ESBLs) in Salmonella enterica serovar Typhi (S. Typhi) from patients with typhoid fever. Objective: To study the antimicrobial resistance and ESBL encoding genes among S. Typhi isolates in aforesaid patients from Lagos, Nigeria. Methods: S. Typhi isolates were collected from blood samples of typhoid fever patients from 4 academic medical centers in Lagos, Nigeria. The identification of isolates and their antibiotic susceptibility testing were performed by standard bacteriological techniques and disc diffusion method, respectively. The production of ESBLs was investigated using combination disk test (CDT) and polymerase chain reaction (PCR). Results: A total of 27 S. Typhi isolates was collected. All isolates were susceptible to imipenem and nitrofurantoin. Fifteen (55.6%) isolates were multidrug-resistant (MDR). The CDT test showed 11 (40.7%) ESBL producer isolates. However, the PCR revealed a higher occurrence rate for ESBL producers (66.7%, n = 18/27). The ESBL genes were as follows:

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“…Meanwhile, Salmonella Kentucky isolation has been increased during recent years (Saroj et al 2009 ; Osman et al 2010a , b ; Abd El-Ghany et al 2012 ; Barua et al 2012 ; Zahran et al 2020 ) and as food borne pathogen both isolated by Shah et al ( 2017 ) Shivaning Karabasanavar et al ( 2020 ), Amin et al ( 2021 ), Gawish et al ( 2021 ), and Adel et al ( 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticle effect on Salmonella enterica isolated from Northern West Egypt food, poultry, and calves

Torky

Khaliel

Sedeek

et al. 2022

Appl Microbiol Biotechnol

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A total no. of 65 Salmonella enterica isolates recovered from food samples, feces of diarrheic calves, poultry, and hospital patient in large five cities at Northern West Egypt were obtained from the Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt. The 65 Salmonella enterica isolates had the invA gene were grouped into 11 Salmonella enterica serovars with dominance of S. Enteritidis and S. Kentucky serovars. Their resistance pattern were characterized by using 18 antibiotics from different classes. Approximately 80% of the isolates were multidrug resistant (MDR). Enterobacterial repetitive intergenic consequences polymerase chain reaction (ERIC-PCR) typing of 7 strains of S. Enteritidis showed 5 clusters with dissimilarity 25%. S. Enteritidis clusters in 2 main groups A and B. Group A have 2 human strain (HE2 and HE3) and one food origin (FE7) with a similarity 99%. Group B divided into B1 (FE2) and B2 (FE3) with a similarity ratio ≥ 93%, while ERIC-PCR analysis of 5 strains of S. Kentucky revealed 4 ERIC types, clustered in 2 main groups A and B with similarity 75%. We studied the effect of silver nanoparticles (Ag-NPs) on 10 antibiotic resistant strains of S. Enteritidis and S. Kentucky. The broth microdilution minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were detected. Evaluation of the affection using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed different ratios of Ag-NPs and microorganism as well as at different contact time ended finally with morphological alteration of the bacteria. We submitted new method in vivo to explore the activity of nanosilver in chicken. Key points • Importance of ERIC-PCR to determine the relatedness between Salmonella isolates. • Effect of silver nanoparticles to confront the antibacterial resistance. • Studying the effect of silver nanoparticles in vivo on infected chicken with Salmonella.

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Prevalence of extended-spectrum β-lactamase (ESBL)-producing Salmonella enterica from retail fishes in Egypt: A major threat to public health

Cited by 16 publications

References 40 publications

Prevalence and molecular characterization of foodborne and human‐derived Salmonella strains for resistance to critically important antibiotics

Prevalence and molecular characterization of foodborne and human‐derived Salmonella strains for resistance to critically important antibiotics

Extended-spectrum beta-lactamases encoding genes among <i>salmonella enterica</i> serovar typhi Isolates in patients with typhoid fever from four Academic Medical Centers in Lagos, Nigeria

Silver nanoparticle effect on Salmonella enterica isolated from Northern West Egypt food, poultry, and calves

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