Prevalence of Fasciola hepatica in the intermediate host Lymnaea truncatula detected by real time TaqMan PCR in populations from 70 Swiss farms with cattle husbandry

Schweizer, G; Meli, Marina L.; Torgerson, Paul R.; Lutz, H; Deplazes, Peter; Braun, Ueli

doi:10.1016/j.vetpar.2007.08.006

Cited by 53 publications

(70 citation statements)

References 10 publications

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“…The enzyme electrophoresis in starch gel is a very cheap and simple biochemical method that could be used in a very Abrous et al (1999Abrous et al ( , 2000, Mage et al (2002) c Vignoles et al (2002) d Barthe (1982, 1987) e Van Aken (1982), Van Aken and Brandt (1987) f Shubkin et al (1992), Rognlie et al (1994Rognlie et al ( , 1996 g Heussler et al (1993), Kaplan et al (1995Kaplan et al ( , 1997 h Krämer and Schnieder (1998), Mostafa et al (2003), Velusamy et al (2004), Cucher et al (2006), Caron et al (2007) i Magalhaes et al (2004) j Heussler et al (1993), Schweizer et al (2007) modest laboratory for a preliminary selection of possible intermediate hosts in a particular epidemiological context but the check out can be difficult (enzyme choice, temperature, chemical revelation). The specificity and sensitivity of the technique is poor when compared with DNA/RNA-based techniques and consequently it is not used anymore.…”

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

“…Real time PCR is difficult to perform particularly with regard to the determination of the experimental conditions of the Abrous et al (1999Abrous et al ( , 2000, Mage et al (2002) c Vignoles et al (2002) d Barthe (1982, 1987) e Van Aken (1982), Van Aken and Brandt (1987) f Shubkin et al (1992), Rognlie et al (1994Rognlie et al ( , 1996 g Heussler et al (1993), Kaplan et al (1995Kaplan et al ( , 1997 h Krämer and Schnieder (1998) Schweizer et al (2007) reaction (design of the TaqMan probe, optimization of E PCR ) and result interpretation. It is an expensive technique because of the TaqMan probe (about 150 Euros), and the price of the fluorescence detection system (about 20,000 Euros).…”

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

“…Recently, a quantitative real time PCR assay was designed and evaluated in naturally infected G. truncatula (Schweizer et al 2007). Primers and probe were designed to amplify an 86-pb-long target of a repetitive 449-bp-long genomic DNA fragment cloned during a previous study (clone Fhr-II, Genbank accession number, S67037) (Heussler et al 1993).…”

Section: Real Time Pcrmentioning

confidence: 99%

“…Primers and probe were designed to amplify an 86-pb-long target of a repetitive 449-bp-long genomic DNA fragment cloned during a previous study (clone Fhr-II, Genbank accession number, S67037) (Heussler et al 1993). The sensitivity of the detection of F. hepatica with this technique was not evaluated (Schweizer et al 2007). Tables 2 and 3 list the advantages and drawbacks of the different methods used to study digenean infections in snails.…”

Section: Real Time Pcrmentioning

confidence: 99%

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The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

2008

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In this review, ten methods used to study digenean infections in their intermediate hosts were compared to determine which one should be used either in the field or in the lab to establish the prevalence and intensity of infections in snails. Snail crushing and snail dissection allow quick establishing of prevalence in natural or experimental infections, whereas histology is considered as the most accurate approach to assess the intensity of infection. The follow-up of cercarial shedding only gave an idea on cercarial production. Among recently developed techniques, polymerase chain reaction (PCR) brings the most accurate information and shows high sensitivity and specificity levels when compared to blotting techniques. The easiness and relatively low cost of the basic PCR protocol make it interesting to investigate the epidemiology of the liver fluke in a lab with limited financial resources. Nevertheless, if this technique allows a relatively good estimation of the prevalence, information concerning the intensity of infection is best obtained through real time PCR. However, at the time being this technique is too expensive to be used routinely in the field. The choice between classical or new techniques is usually based on a compromise, as each technique has its advantages and drawbacks.

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Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

Section: Real Time Pcrmentioning

confidence: 99%

Section: Real Time Pcrmentioning

confidence: 99%

See 2 more Smart Citations

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

2008

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“…A primary objective of many epidemiological studies is to test hypothesized relationships, for example between specific covariates of interest and some response variable, denoting say the presence of, or exposure to, a pathogen or parasite [1][2][3][4][5]. If, however, the method of diagnosis used to classify subjects as disease positive (negative) suffers from imperfect sensitivity and/or specificity then the observed response variable is an estimate of the diagnosis positive fraction of subjects in the study population -typically referred to as apparent prevalence.…”

Section: Introductionmentioning

confidence: 99%

Association between covariates and disease occurrence in the presence of diagnostic error

2011

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SUMMARYIdentification of covariates associated with disease is a key part of epidemiological research. Yet, while adjustment for imperfect diagnostic accuracy is well established when estimating disease prevalence, similar adjustment when estimating covariate effects is far less common, although of important practical relevance due to the sensitivity of such analyses to misclassification error. Case-study data exploring evidence for seasonal differences in Salmonella prevalence using serological testing is presented, in addition simulated data with known properties are analysed. It is demonstrated that : (i) adjusting for misclassification error in models comprising continuous covariates can have a very substantial impact on the resulting conclusions which can then be drawn from any analyses ; and (ii) incorporating prior knowledge through Bayesian estimation can provide potentially more informative assessments of covariates while removing the assumption of perfect diagnostic accuracy. The method presented is widely applicable and easily generalized to many types of epidemiological studies.

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Biology of the Lymnaeidae–Parasite Interaction

Alba,

Gourbal

2023

Zoological Monographs

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Prevalence of Fasciola hepatica in the intermediate host Lymnaea truncatula detected by real time TaqMan PCR in populations from 70 Swiss farms with cattle husbandry

Cited by 53 publications

References 10 publications

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

Association between covariates and disease occurrence in the presence of diagnostic error

Biology of the Lymnaeidae–Parasite Interaction

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