Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam

Nguyen, Sam Thi; Nguyen, Duc T.; Nguyen, Thi Viet Ha; Huynh, Vu Vy; Le, Duc Quyet; Fukuda, Yasuhiro; Nakai, Yutaka

doi:10.1007/s11250-012-0147-8

Cited by 19 publications

(24 citation statements)

References 20 publications

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“…Most human cases have been reported from Binh Dinh, Phu Yen, and Khanh Hoa Provinces in Central Vietnam, which are associated with local endemic animal fascioliasis (33,34,35,47). Recent studies of cattle in Binh Dinh indicated an overall prevalence of 45.3% for Fasciola eggs using a sedimentation method (33,34), 54.9% using a coprological approach, and 72.2% by serological analysis (35). Fascioliasis is clearly hyperendemic in cattle in Vietnam, with attendant risks for the human population.…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

Nguyen

et al. 2012

J Clin Microbiol

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A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of mitochondrial DNA (mtDNA) spanning the region of cox1-trnT-rrnL, two species-specific forward primers were designed, FHF (for F. hepatica) and FGF (for F. gigantica), and a single reverse primer, FHGR (common for both species). Conventional PCR followed by sequencing was applied using species-specific primer pairs to verify the specificity of primers and the identity of Fasciola DNA templates. Duplex PCR (using three primers) was used for testing with the DNA extracted from adult worms, miracidia, and eggs, producing amplicons of 1,031 bp for F. hepatica and 615 bp for F. gigantica. The duplex PCR failed to amplify from DNA of other common liver and intestinal trematodes, including two opisthorchiids, three heterophyids, an echinostomid, another fasciolid, and a taeniid cestode. The sensitivity assay showed that the duplex PCR limit of detection for each Fasciola species was between 0.012 ng and 0.006 ng DNA. Evaluation using DNA templates from 32 Fasciola samples (28 adults and 4 eggs) and from 25 field-collected stools of ruminants and humans revealed specific bands of the correct size and the presence of Fasciola species. This novel mtDNA duplex PCR is a sensitive and fast tool for accurate identification of Fasciola species in areas of distributional and zonal overlap.

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Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

Nguyen

et al. 2012

J Clin Microbiol

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“…The prevalence of cattle fascioliasis has been reported to be 22.0−23.5% in northern Vietnam [21] and 1.4−51.9% in southern Vietnam [20, 34]. In central Vietnam, where the highest number of human cases has been reported, survey on cattle fascioliasis was restricted in south−central Vietnam [33, 35], and no recent survey focusing on central and north−central Vietnam, including the Thua Thien Hue (TTH) province, has been reported. Thus, in the present study, an investigation into the infection of liver flukes ( Fasciola spp.)…”

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confidence: 99%

“…In TTH province, cattle are treated for fascioliasis independently by owner’s will or veterinarian’s suggestion. Most of the fascioliasis surveillance were conducted by fecal examination for Fasciola eggs [20, 21, 33,34,35]. In order to file epidemiological data in TTH province, fecal egg counting was also applied in this study.…”

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confidence: 99%

“…The species of the Lymnaeidae family are known as intermediate hosts for F. gigantica or F. hepatica [3, 27, 35, 43, 46]. In Vietnam, it has been reported that the most frequent intermediate hosts of F. gigantica are Lymnaea swinhoei and L. viridis [20, 35, 43, 57].…”

mentioning

confidence: 99%

“…In Vietnam, it has been reported that the most frequent intermediate hosts of F. gigantica are Lymnaea swinhoei and L. viridis [20, 35, 43, 57]. The habitat preference of snails is lowland plains with slow moving streams; generally, a high number of snails reside in this environment [8], resulting in the high prevalence of cattle fascioliasis [23, 24, 50].…”

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confidence: 99%

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High prevalence of cattle fascioliasis in coastal areas of Thua Thien Hue province, Vietnam

Nguyen

et al. 2017

The Journal of Veterinary Medical Science

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In Vietnam, especially central Vietnam, patients with fascioliasis are increasingly being reported. Since the fascioliasis is zoonotic, survey on the cattle fascioliasis should be informative for the control of human fascioliasis. In this study, the prevalence of cattle fascioliasis as well as the density of the intermediate host snails, Lymnaea swinhoei and L. viridis, were studied in Thua Thien Hue (TTH) province during 2014–2015. A total of 572 cattle feces were examined from 27 communes in 9 districts. Fasciola eggs were detected in cattle from 24 communes with an average prevalence of 23.4% (134/ 572). The highest prevalence was detected in cattle in the coastal plain terrain (31.0%) followed by plain (25.5%), mountain (21.7%), and low hilly (16.2%) terrains. The highest proportion of heavy infection (>200 EPG) was observed in the coastal plain terrain (36.1%), followed by mountains (20.0%), low hills (13.0%), and plains (8.9%). Low number of heavy infection, as well as relatively low prevalence in low hills and plains were associated with the extensive use of anti-fluke treatments. High number of intermediate host snails in low hilly and plain terrains also indicate high risk of fascioliasis. In this study, the density of Lymnaea snails in the coastal plain terrain was found to be very high (17.3 snails/m2) compared to that in previous studies. This is the first report indicating the recent expansion of cattle fascioliasis in the coastal region in Vietnam.

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The transmission of Fasciola spp. to cattle and contamination of grazing areas with Fasciola eggs in the Red River Delta region of Vietnam

Anh

Thanh

Hoan

et al. 2014

Trop Anim Health Prod

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At four times during November 2010, cattle with infections of Fasciola spp., in two communes of northern Vietnam, were allocated to two equivalent groups. Cattle in one group were treated with triclabendazole. Faecal samples collected monthly from both groups were tested for Fasciola copro-antigens and the presence of Fasciola eggs. Re-infection of treated cattle occurred from early March to late November, coinciding with high weekly totals of rainfall. Contamination of grazing areas by untreated cattle was high and relatively constant throughout the year. However, contamination was reduced to undetectable amounts for 8 to 12 weeks after treatment and even at 20 weeks was only 50% or less of the pre-treatment amounts. Therefore, treatments given in mid-September and again in early April, at the start of the wet season, may be sufficient to prevent contamination of grazing areas and reduce the prevalence and severity of Fasciola infections in cattle.

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Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam

Cited by 19 publications

References 20 publications

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

High prevalence of cattle fascioliasis in coastal areas of Thua Thien Hue province, Vietnam

The transmission of Fasciola spp. to cattle and contamination of grazing areas with Fasciola eggs in the Red River Delta region of Vietnam

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