Prevalence of Fimbrial Antigen (K88 variants, K99 and 987P) of Enterotoxigenic Escherichia Coli from Neonatal and Post-weaning Piglets with Diarrhea in Central China

Abstract

Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations were carried out on intensive pig farms in Hubei province, central China. In 2002-2003 fecal samples from neonatal and post-weaning piglets with diarrhea were tested for the presence of the fimbrial antigen K88 and K99 of ETEC by the polymerase chain reaction (PCR). Twenty-three (10.1%) of 227 fecal samples were found to contain fimbrial antigen K88, which was identified as K88ac variant; and 13 (5.7%) samples containing K99. In 2004, another 179 fecal samples from diarrheic piglets, 1 day to 6 weeks of age, were tested for prevalence of fimbrial antigen K88, K99 and 987P. Forty-seven (26.3%) of the 179 samples carried at least one of the ETEC fimbrial antigens. K88 antigen was detected in 20.1%. In the 36 samples known to carry fimbrial antigen K88, 32 (88.9%) contained K88ad; and 4 (11.1%) contained K88ac; none of them carried K88ab. Fimbrial antigens K99 and 987P were detected in 1.1% and 6.1%, respectively. Our data indicate that K88 is the most common fimbrial antigen of ETEC associated with diarrhea in piglets in Central China.

Molecular screening and risk factors of enterotoxigenic Escherichia coli and Salmonella spp. in diarrheic neonatal calves in Egypt

“…The prevalence of ETEC K99 was 10.36%. Similar result was reported previously by Wang et al (2006). However, higher prevalence was reported by Bendali et al (1999) and Achá et al (2004) who reported a prevalence rate of 20.3% and 16%, respectively.…”

The receptor locus for Escherichia coli F4ab/F4ac in the pig maps distal to the MUC4–LMLN region

“…Whereas the two variants F18ab and F18ac recognize the same receptor (Rippinger et al 1995), the three variants of F4 vary in their receptor specificities (Bijlsma et al 1982). However, F4ac is by far the most prevalent variant all over the world with the exception of central China, where F4ad is reported to be predominant (Wang et al 2006). Fortunately, the causative mutation for E. coli F18 susceptibility is known (Meijerink et al 1997(Meijerink et al , 2000 and elimination of the E. coli F18 susceptibility allele from the porcine population is currently done in Switzerland and other countries to increase the number of resistant piglets in commercial populations (Luther et al 2009).…”

Expression optimization of subunitfasGof 987P fimbriae in enterotoxigenicEscherichia coli(ETEC)

“…The 987P-positive bacterium is one of the enterotoxigenic Escherichia coli (ETEC) strains that cause diarrhoea in neonatal piglets (Deannystrom and Samuel, 1994;Wang et al, 2006). It was reported to cause diarrhoea through direct delivery of toxins to host enterocytes after binding to brush borders by means of adhesive proteins in fimbriae (Nataro and Kaper, 1998).…”