Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Abstract

There is not much information available regarding the prevalence of the genotypes of Helicobacter pylori isolates in Turkey, particularly in eastern Turkey. The aims of this study were to detect the prevalence of different genotypes of H. pylori in Turkish patients with gastrointestinal complaints and to determine the relationship of these genotypes with clinical outcome and sex. One hundred forty H. pylori isolates were examined for the presence of its genotypes by the PCR. We found that the prevalence of vacAs1, vacAs2, cagA, cagE, iceA1, iceA2 and babA2 genes were 88.6%, 11.4%, 71.4%, 35.7%, 41.4%, 58.6%, and 62.1%, respectively. The most predominant vacA subtype was s1a (81.4%). The most vacA allelic combination detected were vacAs1m1 (65.2%) and s1m2 (53.9%) in patients with peptic ulcer and gastritis, respectively.The only vacAs1 isolate was significantly associated with gastritis and peptic ulcer (p<0.05). The vacAs1a, ml, slml and babA2 genes were significantly associated with peptic ulcer (p<0.05), whereas m2 gene was significantly associated with only gastritis (p<0.05). The difference between sex and genotypes was statistically significant among the cagA, vacAs1, iceA2 and babA2 genes. This study reported for the first time the prevalence of H. pylori genotypes in patients with gastrointestinal complaints in eastern Turkey.Further studies are needed to understand epidemiological importance of the genotypes of H. pylori isolates in this region and the association between the virulence genes and clinical outcome in different regions.

Helicobacter pylori vacA genotyping in relation to cagA status, ultra-structure of gastric mucosa and clinical outcomes in Egyptian patients

“…In the current study, the vacA s2 allele was the most predominant genotype (55%) followed by vacA s1 (45%). Our findings are similar to an earlier report from Egypt (Van Doon et al, 1999), Korea (Kim et al, 2001) and Turkey (Ozbey and Aygun, 2012). Al Quabandi et al (2005) reported that North Africans were predominantly infected with the s2 type.…”

“…The prevalence of cagA (38%) in this study was in agreement to previous report from Egypt (El-Garbawy et al, 2006) and lower than recent studies from Turkey (71.4%) (Ozbey and Aygun, 2012), Korea (97%) (Kim et al, 2001), Malaysia (94%) (Ramelah et al, 2005) China (96.3%) (Zhou et al, 2004) and Kuwait (53%) (Al Quabadi et al, 2005).…”

INVESTIGATION OF Helicobacter pylori VIRULENCE GENOTYPE IN GASTRIC BIOPSIES BY PCR

“…As previously reported that the iceA1 and the iceA2 genotypes were, respectively, isolated in 60.2% and in 16% of strains [30]. The iceA1 genotype associated with peptic ulceration in patients [2,16,30,58]. In our study, iceA2 allele was considered predominant and there was no association between iceA2 and cagA status.…”

“…The iceA gene contains two allelic variants iceA1 and iceA2 [45,58]. Analysis of iceA gene based on PCR assay using primers specific to sequence region of iceA gene, which give products of 247 bp and/or 229/334 bp for iceA1 and iceA2 respectively, as shown in Fig.…”

Production of a Recombinant CagA Protein for the Detection of Helicobacter pylori CagA Antibodies

“…Ülkemizde cagA gen bölgesine yönelik PCR tabanlı ve anti-CagA tespitine yönelik serolojik araştırmalar yapılmış olup, bunlar daha ziyade prevalans çalışmalarıdır [28][29][30][31][32][33][34][35] . Bizim çalışmamızda üretilen rekombinant CagA proteini, hasta serumlarında anti-CagA antikorlarının araştırılması amacıyla geliştirilecek olan testlerde kullanılabilir niteliktedir.…”