Prevalence of Gram negative bacteria causing neonatal septicemia in a tertiary care hospital of Dehradun, Uttarakhand, India

Raina, Dimple; Jyoti, Rana; Mahawal, Bhim Singh; Khanduri, Ankit

doi:10.20546/ijcmas.2016.501.011

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…A similar trend was noted in study conducted by Cucunawangsih et al where maximum isolates (73.8%) were obtained from age group of 40 to 60 years 13 and in contrast, Dimple and Nupur reported maximum isolation from the age group of <10 years (22.6%) followed by age group of 41 to 50 years (20.8%). 14 The trend seen in our study may be because patients in the age group >40 years have more chances of having comorbid conditions making them more prone to bacterial infections such as MDR Acinetobacter infections.…”

Section: Discussionmentioning

confidence: 64%

“…Similar findings were seen in study by Dimple and Nupur, Tripathi et al, and Rajmane et al reported higher isolation from intensive care units. [14][15][16] Isolation of majority of Acinetobacter species was from LRTIs (59%), and majority of isolates were obtained from respiratory samples (51%) especially ET aspirates (34%). Several other studies show that Acinetobacter species were mainly obtained from respiratory samples.…”

Section: Discussionmentioning

confidence: 99%

“…Similar findings were seen in study by Dimple and Nupur, Tripathi et al, and Rajmane et al reported higher isolation from intensive care units. 14 15 16…”

Section: Discussionmentioning

confidence: 99%

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Role of Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry for Species Identification of Acinetobacter Strains

et al. 2023

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Introduction Acinetobacter species has become a leading cause of nosocomial infections in recent years. Objectives The aim of the study was to establish the usefulness of matrix-assisted laser desorption ionization–time-of-flight (MALDI-TOF) mass spectrometry (MS) for the identification of Acinetobacter species with respect to conventional biochemical methods and MicroScan WalkAway 96 Plus system and to compare the antibiotic susceptibility test results Kirby–Bauer disk diffusion method with MicroScan WalkAway 96 Plus automated identification and antimicrobial susceptibility testing system. Materials and Methods The study sample comprised 100 clinical isolates of Acinetobacter species. They were all identified using MALDI-TOF MS and compared with other two identification systems. Statistical Analysis Comparison of categorical variables by Fisher's exact test or Pearson's chi-square test was done. All statistical tools were two tailed, and a significant level p < 0.05 was used. All statistical tests were performed using SPSS v22.0 (Armonk IBM Corp., New York, United States). Cohen's kappa coefficients were also calculated and used as applicable. Results MALDI-TOF MS revealed 92 A. baumannii, 2 Acinetobacter nosocomialis, 3 Acinetobacter lwoffii, and 1 each was identified as Acinetobacter junii, Acinetobacter johnsonii, and Acinetobacter tandoii. There was moderate agreement between identification by MicroScan WalkAway and MALDI-TOF, and substantial agreement between conventional biochemical tests and MALDI-TOF. We found that there was a 100% categorical agreement with respect to susceptibility of aminoglycosides (amikacin, gentamicin, tobramycin) and cephalosporins (ceftazidime, cefepime, cefotaxime) between disk diffusion method and MicroScan WalkAway 96 Plus system. Total of 16 errors were observed. Conclusion Although MALDI-TOF MS could be useful to identify A. baumannii but not other species in the genus, it is a rapid, reliable method and can be routinely used in clinical laboratories.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

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Role of Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry for Species Identification of Acinetobacter Strains

et al. 2023

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“…The low culture positivity in these studies may be due to intrapartum administration of antibiotics to mothers which can affect the blood culture results in neonate, Various factors like administration of antibiotics to mother or baby before blood collection, possibility of infection with fastidious organisms and anaerobes, etc. pose difficulty in isolation unless advanced automated or molecular techniques are employed, which may be expensive in routine laboratory protocol (1,6,10).…”

Section: Positive Predictive Value 74%mentioning

confidence: 99%

Evaluation of C-Reactive Protein (CRP) in Early Diagnosis of Neonatal Septicemia

Nisarta¹,

Rajat²

2019

Int.J.Curr.Microbiol.App.Sci

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Identification, Speciation and Antibiogram along with Detection of Metallo Beta-lactamase Production in Acinetobacter Isolated from Clinical Samples in a Tertiary Care Hospital

Chitrabanu¹,

Mallya²

2021

J Pure Appl Microbiol

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Acinetobacter species are gram negative non fermenters, which are important nosocomial pathogens involved in various outbreaks in hospitals due to widespread resistance to majority antibiotics. The aim of this study is to speciate Acinetobacter isolated from clinical samples, to assess the antibiotic sensitivity pattern and to detect the production of metallo-β-lactamase by double disc synergy test. The study was conducted in the department of microbiology, A. J Institute of Medical Sciences. All clinical samples were subjected to gram stain & cultured; the Acinetobacter isolates obtained were subjected to antibiogram. Those isolates that showed Imipenem resistant were further tested for production of metallo-β-lactamase by double disc synergy test. Out of 6625 culture positive isolates, 414 (36.1%) were identified biochemically to belong to Acinetobacter species. Of the 414 cases, 393 (94.9%) were further identified to be Acinetobacter baumannii and the remaining 21 (5.1%) to be Acinetobacter lwoffi. Acinetobacter lwoffii showed 100% sensitivity to all the drugs. Of the 393 Acinetobacter baumannii isolates 109 (27.7%) showed resistant to Imipenem. Out of these 109 isolates, 65 (59.63%) were positive for metallo-β-lactamase production by double disk synergy test. The speciation is highly demanding and laborious but it’s important to be demonstrated due to difference in the antibiotic susceptibility pattern. Carbapenem resistant Acinetobacter nosocomial strains in ICUs are detected to be more resistant to antibiotics. As shown in this study the metallo-β-lactamase producing A.baumannii isolates were 59.63% and therapeutic options were limited. Therefore early identification of metallo-β-lactamase producers is of great importance to start appropriate treatment and to control the spread.

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Prevalence of Gram negative bacteria causing neonatal septicemia in a tertiary care hospital of Dehradun, Uttarakhand, India

Cited by 4 publications

References 22 publications

Role of Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry for Species Identification of Acinetobacter Strains

Role of Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry for Species Identification of Acinetobacter Strains

Evaluation of C-Reactive Protein (CRP) in Early Diagnosis of Neonatal Septicemia

Identification, Speciation and Antibiogram along with Detection of Metallo Beta-lactamase Production in Acinetobacter Isolated from Clinical Samples in a Tertiary Care Hospital

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